Alignments for a candidate for actP in Azospirillum brasilense Sp245

GapMind for catabolism of small carbon sources

Alignments for a candidate for actP in Azospirillum brasilense Sp245

Align Cation/acetate symporter ActP; Acetate permease; Acetate transporter ActP (characterized)
to candidate AZOBR_RS02940 AZOBR_RS02940 actetate permease

Query= SwissProt::P32705
         (549 letters)



>FitnessBrowser__azobra:AZOBR_RS02940
          Length = 572

 Score =  754 bits (1946), Expect = 0.0
 Identities = 378/561 (67%), Positives = 463/561 (82%), Gaps = 20/561 (3%)

Query: 7   ALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVVFTLGITYWASKRVRSRSDYYTA 66
           A  A +P + +AA A+ GAV++Q TN+ AI+MFL+FV+ TLGITYWA++R +S  D+Y A
Sbjct: 14  AAGALIPASVHAA-AVEGAVQKQATNFSAIVMFLVFVLATLGITYWAARRTKSAKDFYAA 72

Query: 67  GGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYDGLIYSLGFLVGWPIILFLIAERLR 126
           GG ITGFQNGLAIAGDYMSAASFLGI+ LV+TSG+DGLI+S+G+LVGWPIILFL+AERLR
Sbjct: 73  GGGITGFQNGLAIAGDYMSAASFLGIAGLVYTSGFDGLIFSVGWLVGWPIILFLVAERLR 132

Query: 127 NLGRYTFADVASYRLKQGPIRILSACGSLVVVALYLIAQMVGAGKLIELLFGLNYHIAVV 186
           NLG+YTFADVASYR +Q P+R ++ACGSL  V  YLIAQMVGAGKLI+LLFG++Y  AVV
Sbjct: 133 NLGKYTFADVASYRFQQTPMRTMAACGSLATVTFYLIAQMVGAGKLIQLLFGMDYLWAVV 192

Query: 187 LVGVLMMMYVLFGGMLATTWVQIIKAVLLLFGASFMAFMVMKHVGFSFNNLFSEAMAVHP 246
           +VGVLM+ YV FGGMLATTWVQIIKAVLLL GASFMAF V+   GFS   +FS A+ VHP
Sbjct: 193 IVGVLMIAYVTFGGMLATTWVQIIKAVLLLSGASFMAFAVLAKFGFSPEAMFSTAVQVHP 252

Query: 247 KGVDIMKPGGLVKDPISALSLGLGLMFGTAGLPHILMRFFTVSDAREARKSVFYATGFMG 306
           K   IM PG L+ DP+SA+SLG+ LMFGTAGLPHILMRFFTVSDA+EARKSVFYATGF+G
Sbjct: 253 KATGIMAPGALITDPVSAISLGMALMFGTAGLPHILMRFFTVSDAKEARKSVFYATGFIG 312

Query: 307 YFYILTFIIGFGAIMLV------GANPEYKDAA-----------GHLIGGNNMAAVHLAN 349
           YFYILTFIIGFGAI+L+      GA P + DAA             +IGG+NMAA+H A+
Sbjct: 313 YFYILTFIIGFGAIVLLLAPDATGAYP-FLDAAKLAAAGGKPNPSMIIGGSNMAAIHTAH 371

Query: 350 AVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVFKKG-ATEREELRVSKIT 408
           AVGG+LF GFISAVAFATILAVVAGLTLAGASAVSHDLYA+V  KG A+E +E+RVSKIT
Sbjct: 372 AVGGDLFFGFISAVAFATILAVVAGLTLAGASAVSHDLYASVIAKGRASEHDEIRVSKIT 431

Query: 409 VLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLSMYWSKLTTRGAMMGGWLG 468
            +I+G+++I LG+ FENQN+AFMVGLAF IAAS NFP++L+SM+WS++TTRGA++GGW+G
Sbjct: 432 TVIIGIVSIFLGIAFENQNVAFMVGLAFVIAASANFPVLLMSMFWSRMTTRGAVLGGWIG 491

Query: 469 LITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAFLGIWFFSATDNSAEGARERE 528
           L+++V L+I+GPT+W  +LG+  A+FPY+ P +F+I ++FL IWFFS TDNS     ERE
Sbjct: 492 LVSSVSLLIMGPTVWKSVLGNPAALFPYDNPGVFTIPLSFLAIWFFSITDNSKAAQDERE 551

Query: 529 LFRAQFIRSQTGFGVEQGRAH 549
            ++AQ+IRSQTG G E   AH
Sbjct: 552 AYKAQYIRSQTGLGAEGASAH 572


Lambda     K      H
   0.329    0.142    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1027
Number of extensions: 43
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 549
Length of database: 572
Length adjustment: 36
Effective length of query: 513
Effective length of database: 536
Effective search space:   274968
Effective search space used:   274968
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources