Align The YbhL (AceP) protein. Possibly a pmf-dependent acetate uptake transporter. [14C]Acetate uptake was inhibited by CCCP as well as cold acetate, serine, α-ketoglutarate, lactate, and succinate (M. Inouye, personal communication) (characterized)
to candidate AZOBR_RS07065 AZOBR_RS07065 membrane protein
Query= TCDB::P0AAC4 (234 letters) >FitnessBrowser__azobra:AZOBR_RS07065 Length = 241 Score = 187 bits (475), Expect = 2e-52 Identities = 101/218 (46%), Positives = 149/218 (68%), Gaps = 3/218 (1%) Query: 16 GLQTYMAQVYGWMTVGLLLTAFVAWYAANSAAVMELLFTNRVFLIGLIIAQLALVIVLSA 75 GL+ +M +VY +M +GL +T VA + A++ A+ +FT + + +++A LA ++VLS Sbjct: 26 GLRKHMLRVYNFMMLGLGVTGLVALFVASTPALYVPIFTTPLKWV-VMLAPLAFIMVLSF 84 Query: 76 MIQKLSAGVTTMLFMLYSALTGLTLSSIFIVYTAASIASTFVVTAGMFGAMSLYGYTTKR 135 +S LF + A+ G++++SIF+V+T AS+A F +TA MF AMSL+GYTTK Sbjct: 85 RFHAMSTTALQGLFWAFCAVMGVSMASIFLVFTGASVARVFFITAAMFAAMSLWGYTTKA 144 Query: 136 DLSGFGNMLFMALIGIVLASLVNFWLKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQ 195 DLS G+ L M LIGIV+ASLVN ++ S AL +AV+ IGV++F GLTAYDTQ++K E Sbjct: 145 DLSKMGSFLMMGLIGIVIASLVNIFVGSSALQFAVSVIGVVIFTGLTAYDTQRIKE--EY 202 Query: 196 IDTRDTSNLRKYSILGALTLYLDFINLFLMLLRIFGNR 233 + K +++GAL+LYL+FINLF MLL++ GNR Sbjct: 203 AEGHGHEANTKLAVMGALSLYLNFINLFQMLLQLMGNR 240 Lambda K H 0.330 0.140 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 200 Number of extensions: 11 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 234 Length of database: 241 Length adjustment: 23 Effective length of query: 211 Effective length of database: 218 Effective search space: 45998 Effective search space used: 45998 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory