Align L-arabonate dehydratase (EC 4.2.1.25) (characterized)
to candidate AZOBR_RS31260 AZOBR_RS31260 dihydroxy-acid dehydratase
Query= reanno::BFirm:BPHYT_RS19730 (577 letters) >FitnessBrowser__azobra:AZOBR_RS31260 Length = 608 Score = 471 bits (1212), Expect = e-137 Identities = 271/592 (45%), Positives = 369/592 (62%), Gaps = 27/592 (4%) Query: 11 LRSQAWFGLKDRDGF--LHRSWMKNQGIPHDEFD-GRPVIGICNTWSELTPCNAHFRELA 67 LRS+AWF D L+ N G+ +E G P+IGI T S+L+PCN H LA Sbjct: 19 LRSRAWFDNPDNPDMTALYLERYLNFGLTREELQSGAPIIGIAQTGSDLSPCNRHHLVLA 78 Query: 68 EYVKKGVHEAGGLPLEFPVMSLGETNLRPTAMLFRNLASMDVEESIRGNPMDGVILLVGC 127 E +++G+ AGG+ +EFPV + ET RPTA + RNLA + + E + G P+DGV+L +GC Sbjct: 79 ERLREGIRTAGGIAIEFPVHPIQETGKRPTASIDRNLAYLGLVEVLHGYPLDGVVLTIGC 138 Query: 128 DKTTPALLMGAASCNLPALAVSGGPMLNGRFRGKNIGSGTGVWQMSEEVRAGTMTQEEFT 187 DKTTPA LM AA+ N+PA+A+S GPMLNG FRG+ GSGT VW+ + + AG + + F Sbjct: 139 DKTTPACLMAAATVNIPAIALSVGPMLNGWFRGERTGSGTIVWKARQMMAAGEIDYQGFI 198 Query: 188 EAESCMNRSRGHCMTMGTASTMASMVESLGMGLPHNAAIPAVDARRQVLAHLAGRRIVDM 247 E + S G+C TMGTASTM S+ E LGM LP +AAIPA RQ + G+RIV+M Sbjct: 199 ELVASSAPSTGYCNTMGTASTMNSLAEVLGMQLPGSAAIPAPYRERQQADYETGKRIVEM 258 Query: 248 VREDLTMDKILTRQAFENAIRTNAAIGGSTNAVVHLIALAKRIGVELSLEDWEL-GSNVP 306 VREDL ILTR AF NAI N+AIGGSTNA +H+ A+AK IGV L++EDW+ G +VP Sbjct: 259 VREDLKPSDILTRDAFLNAIVVNSAIGGSTNAPIHINAIAKHIGVPLTVEDWQTHGHDVP 318 Query: 307 CLVNLQPSGEYLMEDFYYAGGLPAVLKQLGEQGLLHKEALTVNGKTLWDNVRNAANYDEK 366 LVNLQP+GEYL EDF+ AGG+PAV+ QL +GL+ + A TVNG+T+ +N R D + Sbjct: 319 LLVNLQPAGEYLGEDFHRAGGVPAVVAQLMGKGLIREGAPTVNGRTIGENCRRQPILDTR 378 Query: 367 VITTFAEPFKPKAGIAVLKGNLAPNGAVIKPSAATASLLKHR------------GRAVVF 414 VI EP P AG VL+GNL GA I ++ + + R G+ VVF Sbjct: 379 VIHPIDEPLMPNAGFVVLRGNLF--GAAIMKTSVISDEFRERYLSNPQDPEAFEGKVVVF 436 Query: 415 ENIEELHAKIDDESLDIDEHCIMVLKGAGPKGYPGFAEVGNMPLPKKVLQKGITDMVRIS 474 + E+ H +IDD SL ID + I+V++G GP GYPG AEV NM P ++++G+ + I Sbjct: 437 DGPEDYHHRIDDPSLGIDAYTILVIRGTGPIGYPGAAEVVNMRPPATLIKQGVHSLPCIG 496 Query: 475 DGRMSGTAYGAVVLHVSPEAAAGGPLAFVQTGDMIELDVEERRLHLDVTDEELARRRAAW 534 DGR SGT+ +L+ SPEAAAGG LA +++GD + +D+ + + + ELA RRAA Sbjct: 497 DGRQSGTSGSPSILNASPEAAAGGGLALLRSGDRVRIDLRRGSADILIPEGELADRRAAL 556 Query: 535 QA----PEAPKRGYYKLYVEHVL-QADQGADLDFLVG----SSGAPVPRDSH 577 +A P P + ++ ++ Q D+G L V + +PRD+H Sbjct: 557 EAAGGYPIPPSQTPWQEIQRGIVDQLDEGMVLKPAVKYQRVAQTMGIPRDNH 608 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 975 Number of extensions: 59 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 577 Length of database: 608 Length adjustment: 37 Effective length of query: 540 Effective length of database: 571 Effective search space: 308340 Effective search space used: 308340 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory