Align Amino acid (Lysine/arginine/ornithine/histidine/octopine) ABC transporter membrane protein, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized)
to candidate AZOBR_RS23520 AZOBR_RS23520 ABC transporter permease
Query= TCDB::Q9HU29 (230 letters) >FitnessBrowser__azobra:AZOBR_RS23520 Length = 220 Score = 145 bits (365), Expect = 8e-40 Identities = 79/218 (36%), Positives = 133/218 (61%), Gaps = 14/218 (6%) Query: 11 MPKMLQGAALTLELLAIAVVAGLALALPLGIAR---ASRHWYVRAVPYAYIFFFRGTPLL 67 +P++L+G +T+E+ A A + L L +GI R A R Y + AY+ F RGTPLL Sbjct: 12 LPELLKGTVVTIEVTAAAFLLSAVLGLLVGIIRLNPARRVLY--GIASAYVAFIRGTPLL 69 Query: 68 LQLFIVYYGLAQFEEVRKSAFWPYLRDPYWCALLTMTLHTAAYIAEILRGAIHSVPVGEV 127 +QLF++++GL QF L C ++ + +++ +Y++E++RGAI S+ G++ Sbjct: 70 VQLFLLFFGLPQFG---------ILLPAMLCGVIGLGIYSGSYVSEVVRGAIQSIDRGQM 120 Query: 128 EAARALGMSRRQALWHIILPRAVRIGLPAYSNEVILMLKASAVVYTVTLFDIMGMARTII 187 EAAR+LGMS R+A+W ++LP+A R LP NE I ++K SA+V +T+ D+M + II Sbjct: 121 EAARSLGMSYREAMWEVVLPQAFRRMLPPLGNETIALIKNSALVSLLTIDDVMREGQRII 180 Query: 188 ARTYESMLFFCLAGALYLVITIVLTRIFRLIERWLRVD 225 + ++ ++ + +Y V+T T I R IE+ + V+ Sbjct: 181 STSFRALEVYIAVALIYFVLTNAATWILRQIEKRMTVE 218 Lambda K H 0.332 0.142 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 142 Number of extensions: 8 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 230 Length of database: 220 Length adjustment: 22 Effective length of query: 208 Effective length of database: 198 Effective search space: 41184 Effective search space used: 41184 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (22.0 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory