Alignments for a candidate for rocA in Azospirillum brasilense Sp245

GapMind for catabolism of small carbon sources

Alignments for a candidate for rocA in Azospirillum brasilense Sp245

Align L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88) (characterized)
to candidate AZOBR_RS09720 AZOBR_RS09720 succinate-semialdehyde dehdyrogenase

Query= BRENDA::Q9RW56
         (523 letters)



>FitnessBrowser__azobra:AZOBR_RS09720
          Length = 497

 Score =  212 bits (539), Expect = 3e-59
 Identities = 147/475 (30%), Positives = 235/475 (49%), Gaps = 23/475 (4%)

Query: 44  IDGQEVDTE-GK-IQSINPCDTSEVVGTTAKATIGDAENALQGAWKAFESWKKWDMDARA 101
           +DG+ +D + GK ++  NP D S V+G+       +   A++ A +A+ +W+      RA
Sbjct: 26  VDGRWIDADSGKTVEVTNPADGS-VLGSVPMMGADETRRAIEAAERAWPAWRALTAKERA 84

Query: 102 RILLKAAAILKRRRLEACALMSIEVGKNYAEADVEVAEAIDFLEYYARSAMKYAGFGSSE 161
           + L     ++   + +   +M+ E GK  AEA  EVA A  F+E++A    +   +G + 
Sbjct: 85  KTLRTWFDLMMANQEDIARIMTAEQGKPLAEARGEVAYAASFIEWFAEEGKRV--YGDTI 142

Query: 162 TTWFEGEENGLMSIPLGVGVSISPWNFPCAIFVGMAAAPIVAGNCVVVKPAEDAGLIAGF 221
                G    +   P+GV  +I+PWNFP A+    A   + AG  +V+KPA    L A  
Sbjct: 143 PQHLPGRRIVVTKEPIGVTAAITPWNFPAAMITRKAGPALAAGCPMVIKPATATPLTALA 202

Query: 222 MVDILREAGLPAGVLQFLPGVGKEVGEYLTTHAKTRFITFTGSRAVGLHINEVAAKVQPG 281
           M  +   AG+PAG+L  + G  + +G  +T +   R +TFTGS  +G  +    A     
Sbjct: 203 MAVLAERAGIPAGILSVVTGSARAIGGEMTGNPTVRKLTFTGSTEIGKELMAQCAGT--- 259

Query: 282 QKWIKRVIMELGGKDGLIVDETADIENAITAATQGAFGFNGQKCSAMSRLIVVDSVYDEV 341
              +K+V +ELGG    +V   AD++ A+  A    +   GQ C   +RL+V   VYD  
Sbjct: 260 ---VKKVSLELGGNAPFLVFNDADLDEAVKGAIASKYRNTGQTCVCANRLLVQSGVYDAF 316

Query: 342 VNGFVERAKALKMGTG--EENANVTAVVNQMSFNKIKGYLELAPSEG-KVLLGGEATGEA 398
                E  KALK+G G   E A    +++  +  K++ ++  A  +G +V+LG    G+ 
Sbjct: 317 AAKLAEAVKALKVGPGLTTEGAQQGPLIDMAAVEKVEDHIRDATEKGARVVLG----GKR 372

Query: 399 NGKQGYYIQPTIVGDVDRNSRLAQEEIFGPVVAVLRAKDWQDALDIANSTEYGLTGGVCS 458
           +   G + +PTI+ DV    ++A+EE FGPV  + R +  ++A+ +AN+TE+GL     S
Sbjct: 373 HELGGSFFEPTILADVTPAMKVAREETFGPVAPLFRFETEEEAVRMANATEFGLAAYFYS 432

Query: 459 NSRERLEQARAEFEVGNLYFNRKITGAIVGVQPFGGYNMSG---TDSKAGGPDYL 510
               R+ +     E G +  N  I      V PFGG   SG     SK G  DYL
Sbjct: 433 RDIGRVWRVAEALEYGIVGINEGIIS--TEVAPFGGMKESGIGREGSKYGIEDYL 485


Lambda     K      H
   0.317    0.134    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 647
Number of extensions: 32
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 523
Length of database: 497
Length adjustment: 34
Effective length of query: 489
Effective length of database: 463
Effective search space:   226407
Effective search space used:   226407
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources