GapMind for catabolism of small carbon sources

 

Alignments for a candidate for bgtA in Azospirillum brasilense Sp245

Align ATPase (characterized, see rationale)
to candidate AZOBR_RS23525 AZOBR_RS23525 ABC transporter ATP-binding protein

Query= uniprot:Q31RN8
         (261 letters)



>FitnessBrowser__azobra:AZOBR_RS23525
          Length = 269

 Score =  245 bits (625), Expect = 8e-70
 Identities = 131/246 (53%), Positives = 170/246 (69%), Gaps = 6/246 (2%)

Query: 21  MIYAEGVEKWYGNQFQALCGVSLTVQRGEVVVMMGPSGSGKSTFLRTLNALESHQRGEIW 80
           +I    V K +G+  + L  VSLTV      V++GPSGSGKST LR  N LE+  RGEI 
Sbjct: 1   VIEIRNVYKSFGST-EVLKDVSLTVPPSRTTVVIGPSGSGKSTLLRCCNCLETADRGEIR 59

Query: 81  IEGHRLSHDRR-----DIATIRQEVGMVFQQFNLFPHLTVLQNLMLAPVQVRRWPVAQAE 135
           I    +  D +     ++  +R E GMVFQ FNLFPH+T ++N+M APV VR    A+A 
Sbjct: 60  INHRTIIADGKPLPDKELNALRAETGMVFQSFNLFPHMTTVENVMRAPVVVRGMAKAEAR 119

Query: 136 ATARQLLERVRIAEQADKYPGQLSGGQQQRVAIARALAMQPRILLFDEPTSALDPEMVRE 195
             A +LL +V + ++AD YP  LSGGQ+QR AIARALAM+P+++LFDEPTSALDPE+V E
Sbjct: 120 ELAMELLRKVGLGDKADVYPSTLSGGQKQRAAIARALAMKPKVMLFDEPTSALDPELVGE 179

Query: 196 VLDVMRDLASEGMTMLVATHEVGFAREVADRVVLMADGQIVEEAPPDRFFTAPQSDRAKQ 255
           VL VM+ LA EGMTM+V THE+GFAREVAD VV+MADG+IVE   P++ FT P  +R + 
Sbjct: 180 VLQVMKTLAEEGMTMMVVTHEMGFAREVADTVVVMADGRIVESGSPEQIFTNPTQERTRG 239

Query: 256 FLAQIL 261
           FL  ++
Sbjct: 240 FLRALV 245


Lambda     K      H
   0.321    0.134    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 217
Number of extensions: 7
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 261
Length of database: 269
Length adjustment: 25
Effective length of query: 236
Effective length of database: 244
Effective search space:    57584
Effective search space used:    57584
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory