GapMind for catabolism of small carbon sources

 

Alignments for a candidate for aapM in Azospirillum brasilense Sp245

Align AapM, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized)
to candidate AZOBR_RS18065 AZOBR_RS18065 amino acid ABC transporter permease

Query= TCDB::Q52814
         (384 letters)



>FitnessBrowser__azobra:AZOBR_RS18065
          Length = 219

 Score =  103 bits (258), Expect = 3e-27
 Identities = 65/208 (31%), Positives = 112/208 (53%), Gaps = 12/208 (5%)

Query: 178 TLVLSFVGIAVSLPVGILLALGRRSRMPVIRMLCVTFIEVIRGVPLITVLFMASVMLPLF 237
           T+VLS V       +G+ L   R  +    RM    ++E+ +G PL+  LF+A   L LF
Sbjct: 20  TVVLSLVSFIGGGLLGMALLYLRIGKARAGRMFVQGYVELFQGTPLLMQLFLAFFGLGLF 79

Query: 238 LPTGWNVDKLLRALIGVSIFTSAYMAEVIRGGLQAIPKGQFEGADSLGLGYWQKTRLIIM 297
              G +V   L A + + ++T+A++ E+ RG ++++ +GQ+E + SLG+GY Q+ R +I+
Sbjct: 80  ---GIDVPAWLAAGLALVLWTAAFLVEIWRGCVESVARGQWEASASLGMGYVQQMRYVIL 136

Query: 298 PQAIKLVIPSIVNTFIGTFKDTSLVTIIGMFDL--LGIVKLNFSDANWASAVTPITGLIF 355
           PQA+++ +P  V   +   K T+L +IIG  +L   G V  N       +   P T    
Sbjct: 137 PQAVRVAVPPTVGFSVQVVKGTALTSIIGFVELSKAGTVVTN-------ATFEPFTVYGL 189

Query: 356 AGFIFWLFCFGMSRYSGFMERHLDTGHK 383
              I++  C+ +S+ S  +ER L+  H+
Sbjct: 190 VALIYFALCWPLSKSSQILERKLNVAHR 217


Lambda     K      H
   0.330    0.145    0.469 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 236
Number of extensions: 10
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 384
Length of database: 219
Length adjustment: 26
Effective length of query: 358
Effective length of database: 193
Effective search space:    69094
Effective search space used:    69094
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory