Align Glutamate/aspartate import permease protein GltK (characterized)
to candidate AZOBR_RS27070 AZOBR_RS27070 ABC transporter permease
Query= SwissProt::P0AER5 (224 letters) >FitnessBrowser__azobra:AZOBR_RS27070 Length = 220 Score = 131 bits (329), Expect = 1e-35 Identities = 70/216 (32%), Positives = 123/216 (56%), Gaps = 7/216 (3%) Query: 1 MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVMRLSSFAPVAWFAKAYV 60 MY F++ + L+G ++T+ +T VV+G+L G + + +LS FA + W + AY+ Sbjct: 1 MYTFNFEMLWGYRWLFLNGTLVTIGLTAAVVVLGLLLGLVGGLAQLSRFAVLRWISWAYI 60 Query: 61 NVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMVAFSMFEAAYYSEIIRAGI 120 +FR PL++ LLWFY +P L+ + ++++ S++ ++Y+E+IR G+ Sbjct: 61 ELFRCTPLLVQLLWFYYALPM-------LTGIQIDAVTASVLTLSLYGGSFYAEVIRGGV 113 Query: 121 QSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLTQGIVLFQDTSLVYVLSLADF 180 SI GQ+ A LALGMT + M+ I+LPQA + M+P L+ Q I+ F++TSLV V+++ D Sbjct: 114 VSIEAGQTEAGLALGMTPAKVMRRIVLPQAVKRMIPPLMNQSIIQFKNTSLVSVVAVPDL 173 Query: 181 FRTASTIGERDGTQVEMILFAGFVYFVISLSASLLV 216 +E+ +YFV+ + + +V Sbjct: 174 LYQGQVAATDTFRPLEVYTIVALIYFVVLVPLTAIV 209 Lambda K H 0.330 0.140 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 157 Number of extensions: 7 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 224 Length of database: 220 Length adjustment: 22 Effective length of query: 202 Effective length of database: 198 Effective search space: 39996 Effective search space used: 39996 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 45 (21.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory