GapMind for catabolism of small carbon sources

 

Alignments for a candidate for atoB in Azospirillum brasilense Sp245

Align acetyl-CoA:acetyl-CoA C-acetyltransferase / acetyl-CoA:propanoyl-CoA 2-C-acetyltransferase (EC 2.3.1.9; EC 2.3.1.16) (characterized)
to candidate AZOBR_RS28180 AZOBR_RS28180 acetyl-CoA acetyltransferase

Query= reanno::pseudo3_N2E3:AO353_25685
         (397 letters)



>FitnessBrowser__azobra:AZOBR_RS28180
          Length = 397

 Score =  474 bits (1220), Expect = e-138
 Identities = 239/389 (61%), Positives = 299/389 (76%)

Query: 6   DPIVIVSAVRTPMGGFQGELKSLSAPQLGAAAIRAAVERAGVAADAVEEVLFGCVLSAGL 65
           DP+VIVSA RTP+G FQGEL +L A  LGA A+RAA+ RAG+A + V+EVL GCVL AG 
Sbjct: 6   DPVVIVSAARTPLGRFQGELSALPAHALGAHAVRAALSRAGLAPERVDEVLLGCVLPAGQ 65

Query: 66  GQAPARQAALGAGLDKSTRCTTLNKMCGSGMEAAILAHDMLLAGSADVVVAGGMESMSNA 125
           GQAPARQAA GAGL  +T  TT+NK+CGSGM+A +LAHD++ AGSAD+VVAGGMESMSNA
Sbjct: 66  GQAPARQAARGAGLPDATGATTVNKVCGSGMKATMLAHDLIRAGSADLVVAGGMESMSNA 125

Query: 126 PYLLDRARSGYRMGHGKVLDHMFLDGLEDAYDKGRLMGTFAEDCAEANGFTREAQDEFAI 185
           PYLL +AR GYR+GH ++LDH+ LDGLEDAY+ GR MG F E  A+  GFTR  QD +A+
Sbjct: 126 PYLLAKARGGYRIGHDRILDHLMLDGLEDAYEGGRPMGDFGEATADLYGFTRAEQDAYAV 185

Query: 186 ASTTRAQQAIKDGSFNAEIVPLQVIVGKEQKLITDDEQPPKAKLDKIASLKPAFRDGGTV 245
            + TRA+ AI  G+F AEI P+ + V   ++ + DDE P K   +KI +LKPAFR  GT+
Sbjct: 186 ETLTRARAAIASGAFTAEIAPVTLAVKGGERTVADDENPLKVAPEKIPALKPAFRRDGTI 245

Query: 246 TAANSSSISDGAAALLLMRRSEAEKRGLKPLAVIHGHAAFADTPGLFPVAPVGAIKKLLK 305
           TAA+SS+ +DGAAALLL RRS AE+ GL  LA I GHA  +  P  F  AP+ AI+KLL 
Sbjct: 246 TAASSSANADGAAALLLTRRSLAEREGLPVLATILGHATHSQDPAWFTTAPIPAIRKLLD 305

Query: 306 KTGWSLDEVELFEVNEAFAVVSLVTMTKLEIPHSKVNVHGGACALGHPIGASGARILVTL 365
           + GW + +V+LFE+NEAFAVV++     L IP   +NV+GGACALGHPIGA+GAR++VTL
Sbjct: 306 RVGWGIGDVDLFEINEAFAVVAMAAQRDLGIPRDALNVNGGACALGHPIGATGARLIVTL 365

Query: 366 LSALRQKGLKRGVAAICIGGGEATAMAVE 394
           L AL  +GL+RGVA++CIGGGEATA+AVE
Sbjct: 366 LHALAARGLRRGVASLCIGGGEATAIAVE 394


Lambda     K      H
   0.318    0.133    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 494
Number of extensions: 12
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 397
Length of database: 397
Length adjustment: 31
Effective length of query: 366
Effective length of database: 366
Effective search space:   133956
Effective search space used:   133956
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory