Alignments for a candidate for puuA in Azospirillum brasilense Sp245

GapMind for catabolism of small carbon sources

Alignments for a candidate for puuA in Azospirillum brasilense Sp245

Align Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) (characterized)
to candidate AZOBR_RS08850 AZOBR_RS08850 glutamine synthetase

Query= reanno::BFirm:BPHYT_RS23160
         (444 letters)



>FitnessBrowser__azobra:AZOBR_RS08850
          Length = 447

 Score =  573 bits (1476), Expect = e-168
 Identities = 269/444 (60%), Positives = 342/444 (77%), Gaps = 3/444 (0%)

Query: 4   IDDFLKKNRVTEIEAIIPDMAGIARGKIIPRSKFES---GESMRLPQAVMIQTVTGDYPE 60
           ++DF+KKNR+TE+E ++PDM+GIARGKI+P  KF        +RLP+A+ +QTVTG+YPE
Sbjct: 4   MEDFIKKNRITEVECLVPDMSGIARGKIVPAEKFLRILRDRGLRLPEAIFVQTVTGEYPE 63

Query: 61  DGTLTGVTDPDMVCVPDASTIRMIPWAVDPTAQVIHDCVHFDGTPVAISPRRVLRRVLEL 120
           D ++T   + D+  +PD  TIR +PW  +PTAQVI DCV+ DG+PV  SPR VL+RVL L
Sbjct: 64  DDSVTSDENSDIYMIPDERTIRFVPWYEEPTAQVITDCVYADGSPVNFSPRHVLKRVLSL 123

Query: 121 YKAKGWKPVIAPELEFYLVDMNKDPDLPLQPPIGRTGRPETGRQAYSIEAVNEFDPLFED 180
           Y+ +GWKP++APELEF+LV +NKDPD PL PP+GR GR E+GRQA+ I+AVNEFDP+FE 
Sbjct: 124 YEERGWKPMVAPELEFFLVQVNKDPDYPLVPPVGRNGRVESGRQAFGIDAVNEFDPIFEM 183

Query: 181 IYEYCEVQELEVDTLIHEVGAAQMEINFMHGDPLKLADSVFLFKRTVREAALRHKMYATF 240
           +Y++CE Q++++DTL HE GAAQ+EINF HGD L+LAD  FLFKRT REAALRH++YATF
Sbjct: 184 VYDFCEAQDIDIDTLTHEAGAAQIEINFNHGDALELADQAFLFKRTAREAALRHQVYATF 243

Query: 241 MAKPMEGEPGSAMHMHQSLVDEETGHNLFTGPDGKPTSLFTSYIAGLQKYTPALMPIFAP 300
           MAKPM+ EPGSAMH+HQS+VD ++G NLF+  +G  T LF S+IAGLQKY P  MP+ AP
Sbjct: 244 MAKPMQNEPGSAMHIHQSVVDADSGRNLFSDANGADTPLFMSHIAGLQKYLPYAMPLLAP 303

Query: 301 YINSYRRLSRFMAAPINVAWGYDNRTVGFRIPHSGPAARRIENRIPGVDCNPYLAIAATL 360
            +NSYRRL     APINV WG DNRT G R+P S   +RR+ENR+ G D NPYLAIAA+L
Sbjct: 304 NVNSYRRLVPNSDAPINVHWGRDNRTTGLRVPVSPADSRRVENRVAGADANPYLAIAASL 363

Query: 361 AAGYLGMTQKLEATEPLLSDGYELPYQLPRNLEEGLTLMGACEPIAEVLGEKFVKAYLAL 420
           A GYLGM Q LE T+P+    Y L + LPR+  + L    AC+P+ EVLGEKF+ A   +
Sbjct: 364 ACGYLGMAQGLEPTDPVKGSAYRLAFTLPRHQGDALQKFNACKPLKEVLGEKFLDAVTCV 423

Query: 421 KETEYEAFFRVISSWERRHLLLHV 444
           KE EYEA+ RVISSWER +LLL+V
Sbjct: 424 KEAEYEAYHRVISSWERENLLLNV 447


Lambda     K      H
   0.321    0.138    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 687
Number of extensions: 26
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 444
Length of database: 447
Length adjustment: 32
Effective length of query: 412
Effective length of database: 415
Effective search space:   170980
Effective search space used:   170980
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources