GapMind for catabolism of small carbon sources

 

Aligments for a candidate for rocD in Azospirillum brasilense Sp245

Align Ornithine aminotransferase; Orn-AT; Lysine aminotransferase; Lys-AT; EC 2.6.1.13; EC 2.6.1.36 (characterized)
to candidate AZOBR_RS19630 AZOBR_RS19630 4-aminobutyrate aminotransferase

Query= SwissProt::Q5JEW1
         (445 letters)



>lcl|FitnessBrowser__azobra:AZOBR_RS19630 AZOBR_RS19630
           4-aminobutyrate aminotransferase
          Length = 428

 Score =  286 bits (732), Expect = 9e-82
 Identities = 160/413 (38%), Positives = 236/413 (57%), Gaps = 18/413 (4%)

Query: 36  LPIVIERGEGIRVYDVDGNVFYDFASGVGVINVGHSHPRVVEAIKKQAEKFTHYSLTDFF 95
           +P+ ++R E   ++DV+GN F DFA G+ V+N GH HP+++EA+K Q ++FTH       
Sbjct: 22  MPVYVDRAENAELWDVEGNRFIDFAGGIAVLNTGHRHPKIIEAVKAQLDRFTHTCAMVTP 81

Query: 96  YENAIILAEKLIELAPGDIERKVVYGNSGAEANEAAMKLVKYGTGRKQFLAFYHAFHGRT 155
           YE+ + LAE+L  L PG   +K  +  +GAEA E A+K+ +  TGR   +AF  AFHGRT
Sbjct: 82  YESFVTLAERLNALVPGSTPKKTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGAFHGRT 141

Query: 156 QAVLSLTASKWVQQDGFFPTMPGVTHIPYPNPYRNTWGIDGYEEPDELTNRVLDFIEEYV 215
              ++LT      + GF P    V H P+PN YR     D            L  +E+  
Sbjct: 142 LLAMALTGKVVPYKVGFGPFPAEVYHAPFPNAYRGVSVQDS-----------LKALEQLF 190

Query: 216 FRHVPPHEIGAIFFEPIQGEGGYVVPPKGFFKALKKFADEYGILLADDEVQMGIGRTGKF 275
              V    + AI  EP+QGEGG+ + P  F +AL+K  DE GILL  DE+Q G  RTGK 
Sbjct: 191 KSDVDATRVAAIIVEPVQGEGGFNIAPPEFLQALRKICDENGILLIIDEIQTGFARTGKM 250

Query: 276 WAIEHFGVEPDLIQFGKAIGGGLPLAGVIHRADI-TFDKPGRHATTFGGNPVAIAAGIEV 334
           +AIEH GVEPDL+   K++ GG PL+ V  +A+I     PG    T+ G+P+A  A + V
Sbjct: 251 FAIEHSGVEPDLMTMAKSLAGGFPLSAVTGKAEIMDAPIPGGIGGTYAGSPLATTAALAV 310

Query: 335 VEIVKE--LLPHVQEVGDYLHKYLEEFKEK--YEVIGDARGLGLAQAVEIVKSKETKEKY 390
           +++++E  L+    ++G+ +        ++    VIGD R LG   A+E+VK + TKE  
Sbjct: 311 LDVIEEEKLIQRSNDLGERIAGRFRTMAQRNTLSVIGDVRNLGGMIAMELVKDRGTKEPA 370

Query: 391 PELRDRIVKESAKRGLVLLGCG--DNSIRFIPPLIVTKEEIDVAMEIFEEALK 441
            EL   +V ++A++GLVLL CG   N IR + PL  +   +D  ++I E +L+
Sbjct: 371 AELTKALVAKAAEKGLVLLSCGTYGNVIRILVPLTASDALVDEGLDIIERSLE 423


Lambda     K      H
   0.320    0.141    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 477
Number of extensions: 28
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 445
Length of database: 428
Length adjustment: 32
Effective length of query: 413
Effective length of database: 396
Effective search space:   163548
Effective search space used:   163548
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory