Align alcohol dehydrogenase (quinone) (EC 1.1.5.5) (characterized)
to candidate AZOBR_RS24625 AZOBR_RS24625 methanol dehydrogenase
Query= BRENDA::Q44002 (739 letters) >FitnessBrowser__azobra:AZOBR_RS24625 Length = 627 Score = 319 bits (817), Expect = 3e-91 Identities = 211/600 (35%), Positives = 299/600 (49%), Gaps = 48/600 (8%) Query: 30 ATMASADDGQGATGEAIIHADDHPGNWMTYGRTYSEQRYSPLDQINRSNVGNLKLAWYLD 89 A++A G + ++I+ + +P NW + Y+ QRYSPLDQI +NV NL++AW Sbjct: 10 ASVAVVAAGSVSANDSILKNEANPNNWASQLGNYAGQRYSPLDQITTNNVKNLQVAWSFS 69 Query: 90 LDTNRGQEGTPLVIDGVMYATTNW-SMMKAVDAAT-GKLLWSYDPRVPGNIADKGCCDTV 147 RG EG P+V+ VMY T + + + A+D G+++W Y+ + CCDTV Sbjct: 70 TGVLRGHEGGPIVVGDVMYIHTPFPNKVFALDLNNPGRVIWKYESVQDPTVIPVMCCDTV 129 Query: 148 NRGAAYWNGKVYFGTFDGRLIALDAKTGKLVWSVNTIPPEAELGKQ-RSYTVDGAPRIAK 206 NRG A +GKV+ D L+ALDAKTGK VW +A+ G + T+ AP + K Sbjct: 130 NRGVAVADGKVFLAQADNTLVALDAKTGKEVW-------KAKNGDHTKGETLTAAPLVVK 182 Query: 207 GRVIIGNGGSEFGARGFVTAFDAETGKVDWRFFTAPNPKNEPDHTASDSVLMNK------ 260 +V +G G EFG RG +TA+D +GK+ WR F+ P + S +++M K Sbjct: 183 DKVFVGISGGEFGVRGHLTAYDTNSGKMVWRAFST-GPDADVKIDPSKTMMMGKPIGQKD 241 Query: 261 -AYQTWSPTGAWTRQGGGGTVWDSIVYDPVADLVYLGVGNGSPWNYKYRS----EGKGDN 315 TW P W R GGGT W YDP +L+Y G GN WN R+ K DN Sbjct: 242 LGVSTW-PGEEWKR--GGGTTWGWYTYDPALNLIYYGTGNPGTWNPTQRAVDKDRAKSDN 298 Query: 316 LFLGSIVALKPETGEYVWHFQETPMDQWDFTSVQQIMTLDLPINGETRHVIVHAPKNGFF 375 + +I A P+TGE W +Q+TP D+WDF V + + D+ +NG+ R V+V+ +NGF Sbjct: 299 KWSMTIFARDPDTGEAKWVYQKTPFDEWDFDGVNENILADINMNGQQRKVLVNFDRNGFA 358 Query: 376 YIIDAKTGEFISGKNY-VYVNWASGLDPKTGRPIYNPDALYTLTGKEWYG----IPGDLG 430 Y +D TGE + + + VNWA+ +D KTGRP D T E P LG Sbjct: 359 YTLDRATGELLVAQKFDPTVNWATDVDMKTGRPNV-VDKYSTFANGEDKNTAAVCPAALG 417 Query: 431 GHNFAAMAFSPKTGLVYIPAQQVPFLYTNQVGGFTPHPDSWNLGLDM--NKVGIPDSPEA 488 + +FSP TGL Y+P + Y P S+ G + V + +P + Sbjct: 418 SKDQQPASFSPDTGLFYVPTNHICMDY-------EPFSVSYTAGQAYVGSTVSMYPTPNS 470 Query: 489 KQAFVKDLKGWIVAWDPQKQAEAW-RVDHKGPWNGGILATGGDLLFQGLANGEFHAYDAT 547 G +AWD W R + W+G L+T G + + G G A D Sbjct: 471 HGGM-----GNFIAWDAAAGKIVWSRPERFAVWSGA-LSTKGGVAYYGTLEGYLVAVDMK 524 Query: 548 NGSDLFHFAADSGIIAPPVTYLANGKQYVAVEVGWGGIYPFFL-GGLARTSGWTVNHSRI 606 +G +L+ F SGII TY NGKQYVAV G GG L GL + +G H+ + Sbjct: 525 DGKELWRFKTPSGIIGNINTYTHNGKQYVAVLSGVGGWAGIGLAAGLTQEAGAQAWHNAV 584 Lambda K H 0.318 0.137 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1877 Number of extensions: 150 Number of successful extensions: 17 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 739 Length of database: 627 Length adjustment: 39 Effective length of query: 700 Effective length of database: 588 Effective search space: 411600 Effective search space used: 411600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory