Alignments for a candidate for BPHYT_RS16930 in Azospirillum brasilense Sp245

GapMind for catabolism of small carbon sources

Alignments for a candidate for BPHYT_RS16930 in Azospirillum brasilense Sp245

Align Arabinose import ATP-binding protein AraG; EC 7.5.2.12 (characterized, see rationale)
to candidate AZOBR_RS31245 AZOBR_RS31245 ABC transporter ATP-binding protein

Query= uniprot:B2SYR5
         (512 letters)



>FitnessBrowser__azobra:AZOBR_RS31245
          Length = 518

 Score =  343 bits (880), Expect = 9e-99
 Identities = 198/511 (38%), Positives = 295/511 (57%), Gaps = 14/511 (2%)

Query: 5   LRFDNIGKVFPGVRALDGVSFDVNVGQVHGLMGENGAGKSTLLKILGGEYQPDS--GRVM 62
           L    I K FPGV+ALD V+  V  G++H L+GENGAGKSTL+K+L G Y   S  G + 
Sbjct: 6   LEMKGITKTFPGVKALDDVNLSVREGEIHALIGENGAGKSTLMKVLSGVYPQGSFDGEIR 65

Query: 63  IDGNEVRFTSAASSIAAGIAVIHQELQYVPDLTVAENLLLGQLPNSLGWVNKREAKRFVR 122
             G    F   A S   GI +IHQEL  VP L++ ENL LG    S G ++   A    R
Sbjct: 66  FRGQPQAFRGIADSERLGIIIIHQELALVPLLSITENLFLGNEQASRGVIDWDAATLRAR 125

Query: 123 ERLEAMGVALDPNAKLRKLSIAQRQMVEICKALLRNARVIALDEPTSSLSHRETEVLFKL 182
           E L  +G+   P   +  + + ++Q+VEI KAL +  +++ LDEPT+SL+  +++ L +L
Sbjct: 126 ELLRLVGLHDPPETLITDIGVGKQQLVEIAKALSKEVKLLILDEPTASLNESDSDALLEL 185

Query: 183 VRDLRADNRAMIYISHRMDEIYELCDACTIFRDGRKIASHPTLEG-VTRDTIVSEMVGRE 241
           +   +A   A I ISH+++EI ++ D  TI RDG  + +    E  V++D I+  MVGR 
Sbjct: 186 LLQFKARGIASILISHKLNEIAKVADRVTILRDGTTVETLDCREAVVSQDRIIRGMVGRA 245

Query: 242 ISDIYNYSARPLGEVRFAAKGIE-------GHALAQPASFEVRRGEIVGFFGLVGAGRSE 294
           +SD Y       G+V F  KG         G  + +  +  VRRGE+VG  GL+GAGR+E
Sbjct: 246 LSDRYPRRTTVPGDVLFEVKGWSADHPAHPGRRVVRDVNLTVRRGEVVGIAGLMGAGRTE 305

Query: 295 LMHLVYGADHKKG--GELLLDGKPIKVRSAGEAIRHGIVLCPEDRKEEGIVAMATVSENI 352
               ++G  + +   G+  LDG+ I V +   A+ +G+    EDRK  G+V    +  N+
Sbjct: 306 FAMSLFGRSYGRNIRGQAFLDGREIDVSTISRAMANGLAYATEDRKHLGLVLDNDIRHNV 365

Query: 353 NISCRRHYLRVGMFLDRKKEAETADRFIKLLKIKTPSRRQKIRFLSGGNQQKAILSRWLA 412
            ++  R   +    +D ++E + A+ F + L+I+     Q+   LSGGNQQK +LS+WL 
Sbjct: 366 TLANLRGVAK-RWVIDHEREVQVAEEFRRRLRIRCADVFQETVNLSGGNQQKVVLSKWLF 424

Query: 413 EPDLKVVILDEPTRGIDVGAKHEIYNVIYQLAERGCAIVMISSELPEVLGVSDRIVVMRQ 472
             D +V+ILDEPTRGIDVGAK+EIY +I QL   G  +V+ISSE+PE+LGV+DRI VM  
Sbjct: 425 -ADPQVLILDEPTRGIDVGAKYEIYTIINQLVAEGRGVVLISSEMPELLGVADRIYVMNA 483

Query: 473 GRISGELTRKDATEQSVLSLALPQSSTALPG 503
           G +  E+   +A+++ ++   +    T + G
Sbjct: 484 GEMVAEMPAAEASQEKIMGAIMRSGETLMSG 514



 Score = 80.5 bits (197), Expect = 1e-19
 Identities = 63/238 (26%), Positives = 117/238 (49%), Gaps = 12/238 (5%)

Query: 250 ARPLGEVRFAAKGIEGHALAQPASFEVRRGEIVGFFGLVGAGRSELMHLVYGADHKKG-- 307
           A P+ E++   K   G       +  VR GEI    G  GAG+S LM ++ G  + +G  
Sbjct: 2   AMPILEMKGITKTFPGVKALDDVNLSVREGEIHALIGENGAGKSTLMKVLSGV-YPQGSF 60

Query: 308 -GELLLDGKPIKVRSAGEAIRHGIVLCPEDRKEEGIVAMATVSENINISCRRHYLRVGMF 366
            GE+   G+P   R   ++ R GI++     +E  +V + +++EN+ +   +    V   
Sbjct: 61  DGEIRFRGQPQAFRGIADSERLGIIII---HQELALVPLLSITENLFLGNEQASRGV--- 114

Query: 367 LDRKKEAETADRFIKLLKIKTPSRRQKIRFLSGGNQQKAILSRWLAEPDLKVVILDEPTR 426
           +D       A   ++L+ +  P     I  +  G QQ   +++ L++ ++K++ILDEPT 
Sbjct: 115 IDWDAATLRARELLRLVGLHDPP-ETLITDIGVGKQQLVEIAKALSK-EVKLLILDEPTA 172

Query: 427 GIDVGAKHEIYNVIYQLAERGCAIVMISSELPEVLGVSDRIVVMRQGRISGELTRKDA 484
            ++      +  ++ Q   RG A ++IS +L E+  V+DR+ ++R G     L  ++A
Sbjct: 173 SLNESDSDALLELLLQFKARGIASILISHKLNEIAKVADRVTILRDGTTVETLDCREA 230


Lambda     K      H
   0.320    0.136    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 681
Number of extensions: 41
Number of successful extensions: 11
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 512
Length of database: 518
Length adjustment: 35
Effective length of query: 477
Effective length of database: 483
Effective search space:   230391
Effective search space used:   230391
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources