Align N-Acetyl-D-Glucosamine phosphotransferase system transporter, component of N-acetyl glucosamine-specific PTS permease, GlcNAc IIBC/GlcNAc I-HPr-IIA (characterized)
to candidate AZOBR_RS32325 AZOBR_RS32325 PTS fructose transporter subunit IIA
Query= TCDB::Q9HXN5 (842 letters) >FitnessBrowser__azobra:AZOBR_RS32325 Length = 846 Score = 463 bits (1191), Expect = e-134 Identities = 298/715 (41%), Positives = 402/715 (56%), Gaps = 37/715 (5%) Query: 139 TNPVEVGAPLLQLSPEKAEQRPANPAPGEGSAQRQVRGRARVAHHGGLHARPAALLRKTA 198 T+P + A L + +P A PA P V V + GLHARPA +TA Sbjct: 141 TDPAALAAALGEDAPVAA---PATPGGDLAERFDWV-----VDYPTGLHARPATAWVETA 192 Query: 199 QGFSSQAELHFAGQVASVDSLVGIMGLGVAEQDEVEVICRGEDSEAALGALLAALASATA 258 + +++ ++ VA +LV ++ LG+ D V V G+D+ AAL + A + TA Sbjct: 193 RASAARIQVRHGDLVADAKALVALLQLGLRAGDSVVVSAEGDDAVAALARMKATITRLTA 252 Query: 259 GAPKDAP----RAIAPGEPARPAAVAGTLAGVCASPGLASGPLARL--GAISLPAD---- 308 DA +A AP P + G+ ASPGLA GP+ L A+S+P + Sbjct: 253 REKADAAAAAQKARAPVRGWTPPNPLPAVPGIAASPGLAIGPVHVLPRAAVSVPDEPVPL 312 Query: 309 -DGRHRPEEQHLALDQALQRVRDDVQGSLQQARLGGDENEAAIFSAHLALLEDPGLLDAA 367 +G R E Q L+ + DD RLG +EAAIF+A +L D L+ A Sbjct: 313 IEGGDRLHEALSLTRQNLKALADDTA-----RRLG--PSEAAIFAAQAEILNDTDLVTLA 365 Query: 368 DMLIDQGVGAAHAWHRAIQAQCEILQALGNLLLAERANDLRDLEKRVLR----VLLGDTA 423 L+ +G G A +WH+A++ L AL N +LA RA DLRD+ +RVL L A Sbjct: 366 CQLMVEGHGVAWSWHQAVERTAAGLAALDNPVLAARAADLRDVGQRVLARIDPALRTGGA 425 Query: 424 PLRVPAGAIVAAREITPSDLAPLVDAGAAGLCMAEGGATSHVAILARSKGLPCLVALGAG 483 P I+ A +++PSD A L A GL A+GG TSH AILAR+ GLP +VA GA Sbjct: 426 PDLPDTPCILIAEDLSPSDTAALDMARVIGLATAQGGPTSHTAILARTLGLPAMVAGGAA 485 Query: 484 LLELEEGRQVVLDAGQGRLELSPDARRLEQVALQVAQREEQRRRQQADAQREALTRDGRR 543 L+EL G +LD GRL LSP A + +A+ E ++ ++A A TRDG Sbjct: 486 LMELANGTPAILDGQSGRLHLSPAAADIADARAWIAREEARKAEEEARRGLPARTRDGHE 545 Query: 544 IEIGANVASPREAAEAFANGADGVGLLRTEFLFLERRAAPDEEEQRNAYQEVLDAMGQRK 603 +EIGANV P + A A + GA+ VGL+RTEFLFLER AP E+EQ Y+ +L A+ R Sbjct: 546 VEIGANVNRPDQVAVALSQGAESVGLMRTEFLFLERGDAPGEDEQYETYRGMLTALEGRP 605 Query: 604 VIIRTIDVGGDKHLDYLPLPVEENPALGLRGIHLGQARPELLDQQLRALLRVEPLERCR- 662 +I+R +D+GGDK + +L LP EENP LG+RG L RPELL+ QLRAL R + Sbjct: 606 LIVRALDIGGDKQVPHLQLPHEENPFLGVRGARLLLRRPELLETQLRALYRAAKDGGAKD 665 Query: 663 -----ILLPMVSEVDELRAIRRRLGELATQLGIERLPELGVMIEVPSAALLADQLAEHAD 717 I+ PM++ + E++A+R + +L +P LG+M+EVP+AA+ AD LA H D Sbjct: 666 GGALSIMFPMITALGEVQALRAACERIRAELDAPAVP-LGIMVEVPAAAIQADVLARHVD 724 Query: 718 FLSIGTNDLSQYALAMDRCHAGLADRIDALHPALLRLIAQTCAGAARHGRWVGVCGALAS 777 F SIGTNDL+QYALA+DR H LA D+LHPA+LRLI T GA RHGRWVGVCG +A Sbjct: 725 FFSIGTNDLTQYALAIDRQHPELAAEADSLHPAVLRLIRLTVEGAERHGRWVGVCGGIAG 784 Query: 778 DPLATPVLVGLGVEELSVGPNLVGEIKTRVRQLDAAECRRHAQALLDLGSARAVR 832 DP +L GLGV ELS+ P + +K R+R D + + AQ LD +A AVR Sbjct: 785 DPFGAALLTGLGVRELSMTPRDIPAVKDRLRGSDLSALKDAAQRALDCETADAVR 839 Lambda K H 0.319 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1784 Number of extensions: 103 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 842 Length of database: 846 Length adjustment: 42 Effective length of query: 800 Effective length of database: 804 Effective search space: 643200 Effective search space used: 643200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 56 (26.2 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory