Align Probable 5-dehydro-4-deoxyglucarate dehydratase; EC 4.2.1.41; 5-keto-4-deoxy-glucarate dehydratase; KDGDH (uncharacterized)
to candidate AZOBR_RS08420 AZOBR_RS08420 dihydrodipicolinate synthase
Query= curated2:A4YNH1 (314 letters) >FitnessBrowser__azobra:AZOBR_RS08420 Length = 291 Score = 107 bits (267), Expect = 3e-28 Identities = 83/274 (30%), Positives = 129/274 (47%), Gaps = 12/274 (4%) Query: 22 VTPFKADYSFDETTYRSNMDWLCGYDVAGLFAAGGTGEFFSLTAAEVPEVVKVAVDETKG 81 +TPFK + DE ++S ++W GL G TGE +L+ E VV++ ++ G Sbjct: 10 LTPFK-NGKVDEAAFQSFVEWQVAQGTHGLVPCGTTGESPTLSHEEHNRVVELCIEAAGG 68 Query: 82 RVPVLAGTG-YGTAIAREIAMSAEKAGADGLLLLPPYLMHAEQEGLAAHVEAVCKSVKIG 140 +VPV+AGTG T A + A+KAGA L++ PY QEGL H +A+ + + Sbjct: 69 KVPVVAGTGSNSTEEAISLTQHAKKAGAAAALVVTPYYNKPTQEGLYQHFKAIHDAADLP 128 Query: 141 VIVYN---RDNAILQPDTLARLCERCPNLVGYKDGIGDIELMTRVYTKMGDRLTYIGGLP 197 +++YN R + T+ARL + PN++G KD D+ R+ +G + G Sbjct: 129 IVIYNIPGRSVVDMSVATMARLA-KLPNIIGVKDATADLARPVRLLQDVGPDFIQLSG-- 185 Query: 198 TAETFALPYLDMGVTTYSSAVFNFVPEFATNFYAAVRKRDHATIHAGLKDFILPLIAIRN 257 + AL + G S N P A RK D AT + +D + PL Sbjct: 186 -EDATALAFNAQGGVGCISVTANIAPAQCAAMQDAWRKGDLATAYK-YRDLLTPLHDSMF 243 Query: 258 RKKGYAVSIIKAGMKVIGRDSGPVRLPLTDLTEA 291 + A +K ++G+ S VRLPL +E+ Sbjct: 244 VETSPAP--VKYAASLLGKSSDEVRLPLVAASES 275 Lambda K H 0.320 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 204 Number of extensions: 6 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 314 Length of database: 291 Length adjustment: 27 Effective length of query: 287 Effective length of database: 264 Effective search space: 75768 Effective search space used: 75768 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory