GapMind for catabolism of small carbon sources

 

Aligments for a candidate for pccB in Azospirillum brasilense Sp245

Align propionyl-CoA carboxylase α subunit (EC 6.4.1.3) (characterized)
to candidate AZOBR_RS22295 AZOBR_RS22295 methylcrotonoyl-CoA carboxylase

Query= metacyc::MONOMER-17283
         (535 letters)



>lcl|FitnessBrowser__azobra:AZOBR_RS22295 AZOBR_RS22295
           methylcrotonoyl-CoA carboxylase
          Length = 535

 Score =  777 bits (2007), Expect = 0.0
 Identities = 372/535 (69%), Positives = 443/535 (82%)

Query: 1   MSIIHSHIQPNSPDFQANFAYHQSLAADLRERLAQIRQGGGAEQRRRHEERGKLFVRDRI 60
           M+++ S + P S +FQ N     +L ADLRE++  I+QGGGA+ R +H  RGKL  R+RI
Sbjct: 1   MTVLKSALNPRSAEFQTNADAMSTLVADLREKVGAIKQGGGAKARDKHLSRGKLLPRERI 60

Query: 61  DTLIDPDSSFLEIGALAAYNVYDEEVPAAGIVCGIGRVAGRPVMIIANDATVKGGTYFPL 120
             L+D  S FLE+  +AAY VYD+++PAAGI+ GIG VAG+  M++ NDATVKGGTYFPL
Sbjct: 61  RQLLDVGSPFLELSQMAAYKVYDDDIPAAGIITGIGSVAGQECMVVVNDATVKGGTYFPL 120

Query: 121 TVKKHLRAQEIARENRLPCIYLVDSGGAYLPLQSEVFPDRDHFGRIFYNQAQMSAEGIPQ 180
           TVKKHLRAQE+A++N LPCIYLVDSGGA LP Q EVFPDRDHFGRIF+NQA MSA+GIPQ
Sbjct: 121 TVKKHLRAQEVAQQNNLPCIYLVDSGGANLPNQDEVFPDRDHFGRIFFNQANMSAQGIPQ 180

Query: 181 IACVMGSCTAGGAYVPAMSDEVVIVKGNGTIFLGGPPLVKAATGEEVTAEELGGADVHTR 240
           IA VMGSCTAGGAYVPAMSDE +IV+  GTIFLGGPPLVKAATGE V+AE+LGGADVH+R
Sbjct: 181 IAVVMGSCTAGGAYVPAMSDEAIIVRNQGTIFLGGPPLVKAATGEVVSAEDLGGADVHSR 240

Query: 241 ISGVADYFANDDREALAIVRDIVAHLGPRQRANWELRDPEPPRYDPREIYGILPRDFRQS 300
            SGV D++A +D  ALA+ R +V++L   +R + +LR+P+ P YDPRE+YG++P D R+ 
Sbjct: 241 TSGVTDHYAMNDAHALAMARKVVSNLNRSKRIDMDLREPQEPAYDPRELYGVIPSDPRKP 300

Query: 301 YDVREVIARIVDGSRLHEFKTRYGTTLVCGFAHIEGFPVGILANNGILFSESALKGAHFI 360
           +DVREVIAR+VDGS L EFK  YGTTLVCGFAHI G+PVGI+ANNGILFSESALKGAHF+
Sbjct: 301 FDVREVIARVVDGSVLDEFKPLYGTTLVCGFAHIFGYPVGIIANNGILFSESALKGAHFV 360

Query: 361 ELCCARNIPLVFLQNITGFMVGKQYENGGIAKDGAKLVTAVSCANVPKFTVIIGGSFGAG 420
           ELCC R IPLVFLQNITGFMVG++YE GGIAKDGAKLVTAV+CA VPKFTVIIGGS+GAG
Sbjct: 361 ELCCQRGIPLVFLQNITGFMVGRKYEAGGIAKDGAKLVTAVACAKVPKFTVIIGGSYGAG 420

Query: 421 NYGMCGRAYQPRQLWMWPNARISVMGGTQAANVLLTIRRDNLRARGQDMTPEEQERFMAP 480
           NYGMCGRAY PR LWMWPN+RISVMGG QAA VL  +RRD + ++G+   PEE+E F AP
Sbjct: 421 NYGMCGRAYSPRFLWMWPNSRISVMGGEQAAGVLAQVRRDAMESQGRSWAPEEEEAFKAP 480

Query: 481 ILAKYEQEGHPYYASARLWDDGVIDPVETRRVLALGLAAAAEAPVQPTRFGVFRM 535
           I  +YE +GHPYYASARLWDDG+IDP +TR VL LGL+A+  APV+ T FGVFRM
Sbjct: 481 IRQQYEDQGHPYYASARLWDDGIIDPADTRMVLGLGLSASLNAPVEKTTFGVFRM 535


Lambda     K      H
   0.322    0.139    0.423 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 990
Number of extensions: 44
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 535
Length of database: 535
Length adjustment: 35
Effective length of query: 500
Effective length of database: 500
Effective search space:   250000
Effective search space used:   250000
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory