GapMind for catabolism of small carbon sources

 

Alignments for a candidate for galdh in Azospirillum brasilense Sp245

Align L-arabinose 1-dehydrogenase / D-galactose 1-dehydrogenase (EC 1.1.1.46; EC 1.1.1.48) (characterized)
to candidate AZOBR_RS29790 AZOBR_RS29790 3-ketoacyl-ACP reductase

Query= reanno::pseudo1_N1B4:Pf1N1B4_412
         (272 letters)



>FitnessBrowser__azobra:AZOBR_RS29790
          Length = 250

 Score =  157 bits (397), Expect = 2e-43
 Identities = 96/253 (37%), Positives = 138/253 (54%), Gaps = 6/253 (2%)

Query: 17  ERLKNKVVLLTGAAQGIGEAIVATFASQQARLVISDIQGEKVEKVAAHWREQGADVVAIK 76
           +RL  KV L+TGAAQG+G A+  TF  +  R+ + DI G+  + VA      G   + I 
Sbjct: 3   KRLDGKVALITGAAQGLGLAMAETFVREGGRVAVVDINGDAAKAVA---ERLGESAIGIA 59

Query: 77  ADVSRQQDLHAMARLAIELHGRIDVLVNCAGVNVFRDPLQ-MTEEDWHRCFAIDLDGAWY 135
           A+V+R  D+       +E  GR+D+LVN AG      P + +TEE++ R FA+++   + 
Sbjct: 60  ANVTRMADVEMTIAATVEKFGRLDILVNNAGSTHANGPFENVTEEEFDRVFALNVKSIYL 119

Query: 136 GCKAVLPQMIEQGIGSIINIASTHSTHIIPGCFPYPVAKHGLLGLTRALGIEYAPKGIRV 195
             KAV+  M  Q  G I+N+ ST      PG   Y   K  +  +T++L +E AP  IRV
Sbjct: 120 YSKAVVATMRAQKSGVILNLGSTAGLRPRPGLVWYNATKGAVHNITKSLALELAPDNIRV 179

Query: 196 NAIAPGYIETQLNVDYWNGFADPHAERQRAFDLHPPRRIGQPIEVAMTAVFLASDEAPFI 255
            A+AP   ET L   +  G  D   +R R   + P  R+GQP +VA  A++LASDEA F+
Sbjct: 180 CALAPVATETPLLATFMGG--DTPEKRARMMGIVPLGRLGQPTDVANAALYLASDEAAFL 237

Query: 256 NASCITIDGGRSV 268
               + IDGGR V
Sbjct: 238 TGVVLEIDGGRCV 250


Lambda     K      H
   0.321    0.138    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 138
Number of extensions: 8
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 272
Length of database: 250
Length adjustment: 25
Effective length of query: 247
Effective length of database: 225
Effective search space:    55575
Effective search space used:    55575
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory