GapMind for catabolism of small carbon sources

 

Aligments for a candidate for etfB in Azospirillum brasilense Sp245

Align butanoyl-CoA dehydrogenase (NAD+, ferredoxin) (subunit 2/3) (EC 1.3.1.109) (characterized)
to candidate AZOBR_RS05480 AZOBR_RS05480 protein fixA

Query= BRENDA::Q18AQ6
         (260 letters)



>lcl|FitnessBrowser__azobra:AZOBR_RS05480 AZOBR_RS05480 protein fixA
          Length = 283

 Score =  204 bits (519), Expect = 2e-57
 Identities = 108/235 (45%), Positives = 154/235 (65%), Gaps = 8/235 (3%)

Query: 1   MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEEAIKLKEEMGAHVTVIT 60
           M+IVVCIKQVPD+ ++++ P T T++R GVP+IINP D   LEEA+++K+++GA VTV+T
Sbjct: 1   MHIVVCIKQVPDSAQIRIHPVTNTIMRQGVPAIINPFDLFSLEEALRIKDKVGARVTVLT 60

Query: 61  MGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALAGALKNI----DFDIIIAG 116
           MGPP A+ +L++AL++GAD  +LLTDR FAG+DT ATS AL  A+K +      D++  G
Sbjct: 61  MGPPMAETSLRKALSLGADDAVLLTDRKFAGSDTLATSYALTSAIKKLSEEEQVDLVFCG 120

Query: 117 RQAIDGDTAQVGPQIAEHLNLPSITY---AEEIKTEGEYVLVKRQFEDCCHDLKVKMPCL 173
           +Q +DGDTAQVGP IA  L    +TY    E I      ++V R+ E     LK  +PC+
Sbjct: 121 KQTVDGDTAQVGPGIATRLGFQQLTYITKIESIDQGAREIVVHRRSEGGVQVLKTALPCM 180

Query: 174 ITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLKGSPTSVFKSF 228
           IT L+  NT R+ K+  ++ A     ++TW       + + +GLKGSPT V K F
Sbjct: 181 ITMLEGTNTIRFGKMDDMFRAARYG-LKTWNKDTTGAEETKVGLKGSPTVVSKVF 234


Lambda     K      H
   0.316    0.135    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 182
Number of extensions: 6
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 260
Length of database: 283
Length adjustment: 25
Effective length of query: 235
Effective length of database: 258
Effective search space:    60630
Effective search space used:    60630
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory