Align ABC-type maltose transporter (EC 7.5.2.1) (characterized)
to candidate AZOBR_RS25595 AZOBR_RS25595 sugar ABC transporter ATP-binding protein
Query= BRENDA::Q8NMV1 (376 letters) >FitnessBrowser__azobra:AZOBR_RS25595 Length = 358 Score = 268 bits (684), Expect = 2e-76 Identities = 158/325 (48%), Positives = 198/325 (60%), Gaps = 13/325 (4%) Query: 1 MATVTFKDASLSYPGAKEPTVKKFNLEIADGEFLVLVGPSGCGKSTTLRMLAGLENVTDG 60 MA VT + S+ + + +LE+ADGEF+ VGPSGCGKST LR++AGLE + G Sbjct: 1 MAGVTLRGVRKSF--GRIEVIHGVDLEVADGEFVAFVGPSGCGKSTLLRLIAGLEEPSGG 58 Query: 61 AIFIGDKDVTHVAPRDRDIAMVFQNYALYPHMTVGENMGFALKIAGKSQDEINKRVDEAA 120 + IG + V P R IAMVFQ+YALYPHMT +NM F L ++ + I +RV AA Sbjct: 59 DLSIGGQRVNDRPPAARGIAMVFQSYALYPHMTAYDNMAFGLTLSRTDKGTIAERVRAAA 118 Query: 121 ATLGLTEFLERKPKALSGGQRQRVAMGRAIVRNPQVFLMDEPLSNLDAKLRVQTRTQIAA 180 L + + L+RKP+ LSGGQRQRVA+GRAIVR PQVFL DEPLSNLDA LRVQ R +IA Sbjct: 119 RLLQIEDLLDRKPRDLSGGQRQRVAIGRAIVREPQVFLFDEPLSNLDAGLRVQMRLEIAK 178 Query: 181 LQRKLGVTTVYVTHDQTEALTMGDRIAVLKDGYLQQVGAPRELYDRPANVFVAGFIGSPA 240 L+ L T +YVTHDQ EA+T+ DRI VL G ++Q G P ELY RP N FVAGFIGSPA Sbjct: 179 LKADLRATMIYVTHDQVEAMTLADRIVVLNAGRVEQAGTPLELYHRPRNRFVAGFIGSPA 238 Query: 241 MNLGTFSVKDGDATSGHARIKLS---PETLA--AMTPEDNGRITIGFRPEALEIIPEGES 295 MN V T G R+ L P +A P +T+G RPE + + G Sbjct: 239 MNF--LDVVSEGLTDGSVRVWLPGGVPLDIAVDGAAPAAGTPLTLGVRPEHVGLADGGAG 296 Query: 296 TDLSIPIKLDFVEELGSDSFLYGKL 320 +I VE LG ++ + L Sbjct: 297 LLATILA----VERLGGETHCHAAL 317 Lambda K H 0.316 0.135 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 431 Number of extensions: 18 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 376 Length of database: 358 Length adjustment: 30 Effective length of query: 346 Effective length of database: 328 Effective search space: 113488 Effective search space used: 113488 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory