Alignments for a candidate for manA in Azospirillum brasilense Sp245

GapMind for catabolism of small carbon sources

Alignments for a candidate for manA in Azospirillum brasilense Sp245

Align mannose-1-phosphate guanylyltransferase (EC 2.7.7.13) (characterized)
to candidate AZOBR_RS33830 AZOBR_RS33830 mannose-1-phosphate guanylyltransferase

Query= BRENDA::P07874
         (481 letters)



>FitnessBrowser__azobra:AZOBR_RS33830
          Length = 481

 Score =  526 bits (1354), Expect = e-154
 Identities = 257/468 (54%), Positives = 327/468 (69%)

Query: 3   PVILSGGSGSRLWPLSRKQYPKQFLALTGDDTLFQQTIKRLAFDGMQAPLLVCNKEHRFI 62
           PVILSGG+GSRLWPLSR  YPKQFL L  + T+ Q+T  R++ D   APL++CN+EHRFI
Sbjct: 12  PVILSGGAGSRLWPLSRASYPKQFLPLASEQTMIQETALRVSSDRFAAPLVICNEEHRFI 71

Query: 63  VQEQLEAQNLASQAILLEPFGRNTAPAVAIAAMKLVAEGRDELLLILPADHVIEDQRAFQ 122
           V EQL A +++   I+LEP GRNTAPAV IAA+ L+  G D L+L++P+DHVI     F 
Sbjct: 72  VAEQLRAASVSPAEIILEPVGRNTAPAVCIAALALLESGEDRLMLVMPSDHVIARSDRFL 131

Query: 123 QALALATNAAEKGEMVLFGIPASRPETGYGYIRASADAQLPEGVSRVQSFVEKPDEARAR 182
           +A+  A  AA  G +V FGI    PETGYGYI+A       + +  V+ FVEKPD A A+
Sbjct: 132 EAVEQAAVAAAAGSLVTFGITPVAPETGYGYIKAGGPLGTGDNIRVVERFVEKPDLATAQ 191

Query: 183 EFVAAGGYYWNSGMFLFRASRYLEELKKHDADIYDTCLLALERSQHDGDLVNIDAATFEC 242
            ++A G Y WNSG+FLF A+ Y+ EL+K +  I + C  A   +  D     ++   F  
Sbjct: 192 TYLADGRYLWNSGIFLFSAAAYVAELEKSNPAIVEACRQARSSAARDLTFCRLEKEAFAA 251

Query: 243 CPDNSIDYAVMEKTSRACVVPLSAGWNDVGSWSSIWDVHAKDANGNVTKGDVLVHDSHNC 302
            P +S+DYAVMEKT +A VV +  GWNDVG+WS++WD+  KD +GNV  GDV++H++ N 
Sbjct: 252 SPSDSVDYAVMEKTDQAAVVAVDMGWNDVGAWSALWDIAEKDESGNVVHGDVVLHNARNS 311

Query: 303 LVHGNGKLVSVIGLEDIVVVETKDAMMIAHKDRVQDVKHVVKDLDAQGRSETQNHCEVYR 362
            V    +LV+V GL+D+VV+ T DA+++A +  VQDVK +V+ L A+ R E   H  VYR
Sbjct: 312 YVRSEEQLVAVAGLDDVVVIATDDAVLVADRKHVQDVKLIVERLKAEKRDEHALHTTVYR 371

Query: 363 PWGSYDSVDMGGRFQVKHITVKPGARLSLQMHHHRAEHWIVVSGTAQVTCDDKTFLLTEN 422
           PWGSY  +D+G RFQVK ITVKPG RLSLQMHHHRAEHWIVV GTA VTC +K  LL EN
Sbjct: 372 PWGSYRGIDIGDRFQVKRITVKPGERLSLQMHHHRAEHWIVVQGTALVTCGEKQELLFEN 431

Query: 423 QSTYIPIASVHRLANPGKIPLEIIEVQSGSYLGEDDIERLEDVYGRTA 470
           QS YIP+ + HRL NPGK+PL +IEVQSGSYLGEDDI R ED YGR A
Sbjct: 432 QSVYIPMGTTHRLENPGKVPLHLIEVQSGSYLGEDDIVRFEDGYGRVA 479


Lambda     K      H
   0.319    0.134    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 638
Number of extensions: 22
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 481
Length of database: 481
Length adjustment: 34
Effective length of query: 447
Effective length of database: 447
Effective search space:   199809
Effective search space used:   199809
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources