Alignments for a candidate for iatA in Azospirillum brasilense Sp245

GapMind for catabolism of small carbon sources

Alignments for a candidate for iatA in Azospirillum brasilense Sp245

Align Inositol transport ATP-binding protein IatA, component of The myoinositol (high affinity)/ D-ribose (low affinity) transporter IatP/IatA/IbpA. The structure of IbpA with myoinositol bound has been solved (characterized)
to candidate AZOBR_RS31210 AZOBR_RS31210 sugar ABC transporter ATP-binding protein

Query= TCDB::B8H229
         (515 letters)



>FitnessBrowser__azobra:AZOBR_RS31210
          Length = 516

 Score =  381 bits (978), Expect = e-110
 Identities = 221/504 (43%), Positives = 315/504 (62%), Gaps = 14/504 (2%)

Query: 3   LLDVSQVSKSFPGVRALDQVDLVVGVGEVHALLGENGAGKSTLIKILSAAHAADAGTVTF 62
           LL +  +SK+F GV+ALD VD  V  GE+HALLGENGAGKSTLIK L+  +  DAGTVT 
Sbjct: 12  LLAIRGLSKAFLGVQALDGVDFTVRHGEIHALLGENGAGKSTLIKTLTGVYQRDAGTVTL 71

Query: 63  AGQVLDPRDAPLRRQQLGIATIYQEFNLFPELSVAENMYLGREPRRLGLVDWSRLRADAQ 122
            G+ + PR      Q+L I T+YQE NL P LSVAEN++LGR+P R GLVD   +R  A+
Sbjct: 72  EGRAIAPRGVE-EAQRLHIGTVYQEVNLLPNLSVAENLFLGRQPMRFGLVDRGAMRRRAR 130

Query: 123 ALLNDLGLPLNPDAPVRGLTVAEQQMVEIAKAMTLNARLIIMDEPTAALSGREVDRLHAI 182
           A+L   GL L+  AP+   +VA QQ+V IA+A+ ++A+++I+DEPTA+L  +EV  L  +
Sbjct: 131 AVLIPYGLTLDVTAPLGRFSVATQQIVAIARAVDMSAKVLILDEPTASLDAQEVAVLFKV 190

Query: 183 IAGLKARSVSVIYVSHRLGEVKAMCDRYTVMRDGRFVASGDVADVEVADMVRLMVGRHVE 242
           +  L++R + +++V+H L +V A+CDR TV+R+GR V     A++   D+V +M+GR +E
Sbjct: 191 MRTLRSRGIGIVFVTHFLDQVYALCDRITVLRNGRLVGERRTAELPRLDLVAMMLGRELE 250

Query: 243 FERRKRRRPPGAVVLKVEGVTPAAPRLSAPGYLRQV---SFAARGGEIVGLAGLVGAGRT 299
               +   P        E   P   R    G  R V       R GE+VGLAGL+G+GRT
Sbjct: 251 AVAHRIAPPADDA---EEDARPPLVRFRGYGKARSVEPFDLDIRPGEVVGLAGLLGSGRT 307

Query: 300 DLARLIFGADPIAAGRVLVDDKPLRLRSPRDAIQAGIMLVPEDRKQQGCFLDHSIRRNLS 359
           + ARL+FG D    G   VD + +RLR PRDAI+ G    PEDRK++G     S+R N+ 
Sbjct: 308 ETARLVFGMDRADRGEAAVDGQAVRLRGPRDAIRLGFGFCPEDRKKEGIVGALSVRENII 367

Query: 360 LPSLKALSALGQW---VDERAERDLVETYRQKLRIKMADAETAIGKLSGGNQQKVLLGRA 416
           L    AL A   W   +    + ++ + + + L I+   AE  I  LSGGNQQK LL R 
Sbjct: 368 L----ALQARQGWLRPIPRCRQEEIADRFIRLLDIRTPHAEQPIQLLSGGNQQKALLARW 423

Query: 417 MALTPKVLIVDEPTRGIDIGAKAEVHQVLSDLADLGVAVVVISSELAEVMAVSDRIVVFR 476
           +A  P++LI+DEPTRGID+GA AE+ +++  L   G+A++V+SSEL E++A S R+VV R
Sbjct: 424 LATEPRLLILDEPTRGIDVGAHAEIIRLIERLCADGMALLVVSSELEEIVAYSRRVVVLR 483

Query: 477 EGVIVADLDAQTATEEGLMAYMAT 500
           +   VA+L       + ++A +A+
Sbjct: 484 DRRHVAELRGGEVAVDRIVAAIAS 507



 Score = 72.8 bits (177), Expect = 3e-17
 Identities = 71/251 (28%), Positives = 112/251 (44%), Gaps = 25/251 (9%)

Query: 11  KSFPGVRALDQVDLVVGVGEVHALLGENGAGKSTLIKILSAAHAADAGTVTFAGQVL--- 67
           + +   R+++  DL +  GEV  L G  G+G++   +++     AD G     GQ +   
Sbjct: 275 RGYGKARSVEPFDLDIRPGEVVGLAGLLGSGRTETARLVFGMDRADRGEAAVDGQAVRLR 334

Query: 68  DPRDA--------PLRRQQLGIATIYQEFNLFPELSVAENMYLGREPRRLGLVDWSRLR- 118
            PRDA        P  R++ GI            LSV EN+ L  + R+  L    R R 
Sbjct: 335 GPRDAIRLGFGFCPEDRKKEGIVGA---------LSVRENIILALQARQGWLRPIPRCRQ 385

Query: 119 ---ADAQALLNDLGLPLNPDAPVRGLTVAEQQMVEIAKAMTLNARLIIMDEPTAALSGRE 175
              AD    L D+  P + + P++ L+   QQ   +A+ +    RL+I+DEPT  +    
Sbjct: 386 EEIADRFIRLLDIRTP-HAEQPIQLLSGGNQQKALLARWLATEPRLLILDEPTRGIDVGA 444

Query: 176 VDRLHAIIAGLKARSVSVIYVSHRLGEVKAMCDRYTVMRDGRFVASGDVADVEVADMVRL 235
              +  +I  L A  ++++ VS  L E+ A   R  V+RD R VA     +V V  +V  
Sbjct: 445 HAEIIRLIERLCADGMALLVVSSELEEIVAYSRRVVVLRDRRHVAELRGGEVAVDRIVAA 504

Query: 236 MVGRHVEFERR 246
           +    V  E R
Sbjct: 505 IASESVPEEPR 515


Lambda     K      H
   0.320    0.136    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 714
Number of extensions: 41
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 515
Length of database: 516
Length adjustment: 35
Effective length of query: 480
Effective length of database: 481
Effective search space:   230880
Effective search space used:   230880
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources