GapMind for catabolism of small carbon sources

 

Alignments for a candidate for fadB in Azospirillum brasilense Sp245

Align GDP-6-deoxy-D-talose 4-dehydrogenase (EC 1.1.1.135); 3-hydroxy-2-methylbutyryl-CoA dehydrogenase (EC 1.1.1.178) (characterized)
to candidate AZOBR_RS29790 AZOBR_RS29790 3-ketoacyl-ACP reductase

Query= BRENDA::Q99714
         (261 letters)



>FitnessBrowser__azobra:AZOBR_RS29790
          Length = 250

 Score =  125 bits (315), Expect = 7e-34
 Identities = 89/257 (34%), Positives = 131/257 (50%), Gaps = 20/257 (7%)

Query: 6   RSVKGLVAVITGGASGLGLATAERLVGQGASAVLLDLPNSGGEAQAKKLGNNCVFAPADV 65
           + + G VA+ITG A GLGLA AE  V +G    ++D+     +A A++LG + +   A+V
Sbjct: 3   KRLDGKVALITGAAQGLGLAMAETFVREGGRVAVVDINGDAAKAVAERLGESAIGIAANV 62

Query: 66  TSEKDVQTALALAKGKFGRVDVAVNCAGIAVASKTYNLKKGQTHTLEDFQRVLDVNLMGT 125
           T   DV+  +A    KFGR+D+ VN AG   A+  +     +  T E+F RV  +N+   
Sbjct: 63  TRMADVEMTIAATVEKFGRLDILVNNAGSTHANGPF-----ENVTEEEFDRVFALNVKSI 117

Query: 126 FNVIRLVAGEMGQNEPDQGGQRGVIINTASVAAFEGQVGQAAYSASKGGIVGMTLPIARD 185
           +   + V   M      +  + GVI+N  S A    + G   Y+A+KG +  +T  +A +
Sbjct: 118 YLYSKAVVATM------RAQKSGVILNLGSTAGLRPRPGLVWYNATKGAVHNITKSLALE 171

Query: 186 LAPIGIRVMTIAPGLFGTPLLTSL-----PEKVCNFLASQVPFPSRLGDPAEYAH--LVQ 238
           LAP  IRV  +AP    TPLL +      PEK    +   VP   RLG P + A+  L  
Sbjct: 172 LAPDNIRVCALAPVATETPLLATFMGGDTPEKRARMMGI-VPL-GRLGQPTDVANAALYL 229

Query: 239 AIIENPFLNGEVIRLDG 255
           A  E  FL G V+ +DG
Sbjct: 230 ASDEAAFLTGVVLEIDG 246


Lambda     K      H
   0.318    0.135    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 175
Number of extensions: 7
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 261
Length of database: 250
Length adjustment: 24
Effective length of query: 237
Effective length of database: 226
Effective search space:    53562
Effective search space used:    53562
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory