GapMind for catabolism of small carbon sources

 

Aligments for a candidate for AZOBR_RS08235 in Azospirillum brasilense Sp245

Align L-proline and D-alanine ABC transporter, permease component 1 (characterized)
to candidate AZOBR_RS32415 AZOBR_RS32415 branched-chain amino acid ABC transporter permease

Query= reanno::azobra:AZOBR_RS08235
         (301 letters)



>lcl|FitnessBrowser__azobra:AZOBR_RS32415 AZOBR_RS32415
           branched-chain amino acid ABC transporter permease
          Length = 305

 Score =  198 bits (504), Expect = 1e-55
 Identities = 119/305 (39%), Positives = 180/305 (59%), Gaps = 24/305 (7%)

Query: 3   YFLQQLINGLSLGAIYGLIAIGYTMVYGIIGMINFAHGEIYMIGAFVALITFLAIGSLGI 62
           +FLQQ+INGLS+G +Y L+AIG+T+++G++ ++NFAHGE+Y IGAFV L+   A+    +
Sbjct: 6   FFLQQVINGLSIGCVYALMAIGFTLIFGVLNVVNFAHGEVYTIGAFVGLMVITAMAPPLL 65

Query: 63  TWVPLALLVMLVASMLFTAVYGWTVERIAYRPLRS----------SPRLAPLISAIGMSI 112
             VPL L V         AV G  +ERIA+RP R           + R A L+S++ +SI
Sbjct: 66  AVVPLVLAV--------GAVSGVGLERIAFRPFRRFTDEASQKSRAMREATLLSSLAVSI 117

Query: 113 FLQNYVQILQGARSKPLQPILPGNLTLMDGAVSVSYVRLATIVI--TIALMYGFTQ-LIT 169
             +  +  + G     +Q I  G L     A+    V   ++VI  T A+M G  Q L+ 
Sbjct: 118 MTREIMMHIFGG---DMQGIPSGYLLQQPVAIGPIMVASGSLVIFATSAVMLGALQFLLY 174

Query: 170 RTSLGRAQRACEQDKKMAGLLGVNVDRVISLTFVMGAALAAVAGMMVLLIYGVIDFYIGF 229
           RT  G   RA   ++  A  +G+N DR I  TF +G+ L A AG++V L  G I  ++GF
Sbjct: 175 RTQTGLGIRAVSNNQLGARYVGINTDRTIVTTFAVGSMLGATAGILVGLYDGAISPHMGF 234

Query: 230 LAGVKAFTAAVLGGIGSLPGAMLGGVVIGLIEAFWSGYMGSEWKDVATFTILVLVLIFRP 289
             GVKAF A V+GG+ S+PGA+   +++G+ E+  + ++ S WKD+ T+++LV+ L+F P
Sbjct: 235 APGVKAFVAMVMGGLSSIPGAVACALLLGVSESIATEFLSSGWKDLITYSLLVITLVFFP 294

Query: 290 TGLLG 294
            GL G
Sbjct: 295 QGLFG 299


Lambda     K      H
   0.329    0.144    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 302
Number of extensions: 15
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 301
Length of database: 305
Length adjustment: 27
Effective length of query: 274
Effective length of database: 278
Effective search space:    76172
Effective search space used:    76172
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory