Alignments for a candidate for betS in Azospirillum brasilense Sp245

GapMind for catabolism of small carbon sources

Alignments for a candidate for betS in Azospirillum brasilense Sp245

Align Glycine betaine/proline betaine transporter BetS (characterized)
to candidate AZOBR_RS23220 AZOBR_RS23220 beta-aspartyl peptidase

Query= SwissProt::G3XCN6
         (706 letters)



>FitnessBrowser__azobra:AZOBR_RS23220
          Length = 662

 Score =  528 bits (1361), Expect = e-154
 Identities = 284/627 (45%), Positives = 385/627 (61%), Gaps = 28/627 (4%)

Query: 53  VNLPVFVGSVAVIALFVGIGVIAPKRAESIFSGMQTAILSGFGWLYLLSVAVFLFSMLFL 112
           +N  V   +  +I LFV +  +  +      S +Q+AI+  FGW Y+LSVA FL    +L
Sbjct: 4   INKTVSFTAGGLIVLFVLLASVFTEPFGERISALQSAIVGNFGWFYILSVAGFLLFAFWL 63

Query: 113 AFSRYGELKLGPDDSEPEFRYLSWIAMLFAAGMGIGLMYFAVGEPMTHFASPPEAEPLTI 172
            FS YG +KLG DD EPEF YL+W AMLF+AGMGIGL+++ V EP+ H A+P   E  T 
Sbjct: 64  FFSPYGSIKLGKDDDEPEFSYLTWFAMLFSAGMGIGLLFYGVAEPVLHLANPRVGEAGTP 123

Query: 173 AAQREAMSVTFFHWGVHAWAIYSVVGLSLAYFGYRYNLPLTVRSGLYPLLKEGIHGPIGH 232
            A REAM++ F HWG+HAW IY  VGLSL YF YR++LPLT+RS LYPLL + + G  GH
Sbjct: 124 DAAREAMNLAFLHWGLHAWGIYITVGLSLGYFAYRHDLPLTIRSALYPLLGDRLSGWPGH 183

Query: 233 VVDIFAICGTMFGLATSLGFGILQINSGLNYLLGIPQSIYVQLLLVTVVTAIATISVVTG 292
           +VDI AI GT+FG+ATSLG G++QIN+GL+YL  +      Q++L+ V+TAIAT S V+G
Sbjct: 184 LVDIVAIVGTLFGIATSLGLGVMQINAGLDYLGLVSVGTVQQMVLIAVITAIATASAVSG 243

Query: 293 VEKGVRILSETNLFLAVLLMLFVLVVGPTGTLMRDFVQNIGLYLDSLVLRTFNIYAYEPR 352
           V +G+R LSE N+   +LL+LFV ++GP+  L+   V++IG YL +L   +F    Y   
Sbjct: 244 VGRGIRRLSELNMLAGLLLLLFVFLLGPSVFLLSTLVESIGRYLWTLPYTSFRTLPYTGA 303

Query: 353 PWIDSWTLFYWAWWISWSPFVGMFIARISRGRTVREFVTAVLFVPAMFTFLWMTVFGNTA 412
            W  SWT+FYW WWISW+PFVGMFIAR+SRGRT+REF+  VLF P   TF+W TVFG TA
Sbjct: 304 EWQASWTMFYWGWWISWAPFVGMFIARVSRGRTIREFIGGVLFAPVALTFVWFTVFGETA 363

Query: 413 IYVDTTIANGELARDVKADLSVALFQFFEYLPWPAVTSTLAVLLVSIFFVTSSDSGSLVI 472
           I+++     G +A  V+ ++  ALF   + LP   +TS LA L+V  FFVTS+DSG+LVI
Sbjct: 364 IHME-MFEGGGMAAAVQENVPTALFVMLDRLPLSVITSALATLIVVTFFVTSADSGALVI 422

Query: 473 DTIASGGETATPALQRIFWCSLSGIVAAVLLSTGGLTALQSATISTALPFSLVMLILVWS 532
           D I SGG    P   RIFW  L G+VAAVLL  GGL ALQ+A ++TALPF++VM+++   
Sbjct: 423 DIIGSGGNQDPPIATRIFWAVLCGVVAAVLLLVGGLQALQTAAVTTALPFAVVMVLMCVG 482

Query: 533 LFVGMRAD--------LARTQSPGSLGPRAYPASGVPWQRRLAMTL-------------S 571
           L   +RA+        +AR  +    G    P     W+++LA  +              
Sbjct: 483 LVTSLRAERRAGPDRRVARVPTASEGGAPVQPVGDGDWRQQLAAVIGRKAAIAAAVPPGG 542

Query: 572 TPDRRAVEKFLQASVLPALEAVARELTRRSRPASVGRDAETGALT--LTVPAEGHRDFVY 629
              RR V +F+  +V PA   +A EL R  R A +    E G     L V  +G  +F Y
Sbjct: 543 AGARREVARFIADTVEPAFRDIAAELERLGRRAVI----EVGPFNAGLAVLRDGEEEFSY 598

Query: 630 GVQMSEHKLPAFTAYDATVADVRYEAR 656
            ++   +    F   D   AD +++ R
Sbjct: 599 DIRARAYHPLTFAFPDLKAADEQWQMR 625


Lambda     K      H
   0.326    0.139    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1329
Number of extensions: 77
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 706
Length of database: 662
Length adjustment: 39
Effective length of query: 667
Effective length of database: 623
Effective search space:   415541
Effective search space used:   415541
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources