GapMind for catabolism of small carbon sources

 

Aligments for a candidate for hutV in Azospirillum brasilense Sp245

Align HutV aka HISV aka R02702 aka SMC00670, component of Uptake system for hisitidine, proline, proline-betaine and glycine-betaine (characterized)
to candidate AZOBR_RS15760 AZOBR_RS15760 ATP-binding protein

Query= TCDB::Q9KKE1
         (275 letters)



>lcl|FitnessBrowser__azobra:AZOBR_RS15760 AZOBR_RS15760 ATP-binding
           protein
          Length = 312

 Score =  184 bits (466), Expect = 3e-51
 Identities = 102/236 (43%), Positives = 149/236 (63%), Gaps = 6/236 (2%)

Query: 33  ILSRSGCTVGLNDVSLKIGAGKIFVIMGLSGSGKSTLVRHINRLIEPTSGEVLFDGDNIL 92
           +  R G    ++D+SL + AG+  V++G SGSGKST++R +NRLI P +G +  +G++I 
Sbjct: 7   VTKRFGAWTAVDDLSLTVAAGEFRVLIGPSGSGKSTVLRMMNRLIAPEAGTIRVEGEDIA 66

Query: 93  DLGAKALRAFRMRRVSMVFQSFALMPHRTVLQNVVYGQRVRGVSKDDAREIGMKWIDTVG 152
            L  + LR    RR+  V QS  L PH TV +N+     + G  +   R+   + +  + 
Sbjct: 67  RLKPETLR----RRMGYVIQSVGLFPHWTVERNIAAVPDLLGWPRARVRDRVTELLALLN 122

Query: 153 LSG--YDAKFPHQLSGGMKQRVGLARALAADTDVILMDEAFSALDPLIRGDMQDQLLQLQ 210
           L    Y   +PH+LSGG +QRVG+ARALAA+  ++LMDE FSALDP+ RG +Q ++  + 
Sbjct: 123 LDPERYRGAYPHELSGGQQQRVGVARALAAEPHILLMDEPFSALDPITRGALQAEMAAIH 182

Query: 211 RNLAKTIVFITHDLDEALRIGSEIAILRDGQVVQVGTPNDILDNPANDYVARFVQR 266
           +    TIVF+THD+DEAL + S IA+L  G++VQ GTP DIL +PA+D V   V R
Sbjct: 183 KATGTTIVFVTHDMDEALALASRIAVLDRGRLVQSGTPLDILTDPADDRVRDLVGR 238


Lambda     K      H
   0.323    0.139    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 216
Number of extensions: 11
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 275
Length of database: 312
Length adjustment: 26
Effective length of query: 249
Effective length of database: 286
Effective search space:    71214
Effective search space used:    71214
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory