GapMind for catabolism of small carbon sources

 

Alignments for a candidate for hutV in Azospirillum brasilense Sp245

Align HutV aka HISV aka R02702 aka SMC00670, component of Uptake system for hisitidine, proline, proline-betaine and glycine-betaine (characterized)
to candidate AZOBR_RS22795 AZOBR_RS22795 spermidine/putrescine ABC transporter ATPase

Query= TCDB::Q9KKE1
         (275 letters)



>FitnessBrowser__azobra:AZOBR_RS22795
          Length = 358

 Score =  170 bits (431), Expect = 4e-47
 Identities = 97/232 (41%), Positives = 137/232 (59%), Gaps = 6/232 (2%)

Query: 33  ILSRSGCTVGLNDVSLKIGAGKIFVIMGLSGSGKSTLVRHINRLIEPTSGEVLFDGDNIL 92
           I  R G +  +++V+L+I AG++  ++G SG GK+TL+R +      T G V+  GD ++
Sbjct: 6   ITHRFGASTAVDNVTLEIKAGELVALLGPSGCGKTTLLRILAGFQAQTLGRVVI-GDRVV 64

Query: 93  DLGAKALRAFRMRRVSMVFQSFALMPHRTVLQNVVYGQRVRGVSKDDAREIGMKWIDTVG 152
           D    A R      V +VFQ++AL PH TV QNV YG   RG ++D  R      +  V 
Sbjct: 65  DSLPPAGRG-----VGIVFQNYALFPHMTVAQNVAYGLEARGAARDAVRARVEAMLGLVK 119

Query: 153 LSGYDAKFPHQLSGGMKQRVGLARALAADTDVILMDEAFSALDPLIRGDMQDQLLQLQRN 212
           L     +FP QLSGG +QRV LARALA    ++L+DE F+ALD  +R DMQ ++ QLQR 
Sbjct: 120 LDAMRDRFPKQLSGGQQQRVALARALAIQPSILLLDEPFAALDKNLRLDMQIEIKQLQRR 179

Query: 213 LAKTIVFITHDLDEALRIGSEIAILRDGQVVQVGTPNDILDNPANDYVARFV 264
              T + +THD +EAL +   IA+L  G++ Q GTP D+ D P   +V  FV
Sbjct: 180 FGITTIMVTHDQEEALSMADRIAVLSRGKLEQFGTPEDVYDRPGTLFVNGFV 231


Lambda     K      H
   0.323    0.139    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 212
Number of extensions: 12
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 275
Length of database: 358
Length adjustment: 27
Effective length of query: 248
Effective length of database: 331
Effective search space:    82088
Effective search space used:    82088
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory