GapMind for catabolism of small carbon sources

 

Aligments for a candidate for natD in Azospirillum brasilense Sp245

Align NatD, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized)
to candidate AZOBR_RS29670 AZOBR_RS29670 ABC transporter permease

Query= TCDB::Q8YXD0
         (288 letters)



>lcl|FitnessBrowser__azobra:AZOBR_RS29670 AZOBR_RS29670 ABC
           transporter permease
          Length = 290

 Score =  171 bits (432), Expect = 2e-47
 Identities = 95/281 (33%), Positives = 159/281 (56%), Gaps = 4/281 (1%)

Query: 6   IQLIVNGIAVGSIIALAAVGLTLTYGILRLSNFAHGDFLTLGAYLTFFV-NTFGVNIWLS 64
           +Q ++N + +G   AL  +GLTL +GI+R+ NF HG+  T GAY+ + +    G+N ++S
Sbjct: 5   LQHLLNAVVLGGTYALLGIGLTLIFGIMRVVNFTHGELYTFGAYMAYMLAGMMGLNFFMS 64

Query: 65  MIVAVVGTVGVMLLSEKLLWSRMRSIRANSTTLIIISIGLALFLRNGIILIWGGRNQNYN 124
           + +A V  + +  L E  L   ++    ++T L++I  G+A+  + G  L+WGG  ++  
Sbjct: 65  LAMAAVLGMALGALIEFTLLRPLKGADIDTTMLVMIGAGIAM--QAGEQLVWGGVAKSVP 122

Query: 125 LPITPALDIFG-VKVPQNQLLVLALAVLSIGALHYLLQNTKIGKAMRAVADDLDLAKVSG 183
            P      + G V V  N+L VL +A+L +G  + L+  TK+G AMRA   D D A + G
Sbjct: 123 SPFPTEPVVLGSVSVGMNRLFVLGVALLLLGGFYLLINRTKLGVAMRATFQDPDTAALMG 182

Query: 184 IDVEQVIFWTWLIAGTVTSLGGSMYGLITAVRPNMGWFLILPLFASVILGGIGNPYGAIA 243
           ++   +   T+ +   + +  G++ G I  V P MG  + L  FA VILGG+GN  GA  
Sbjct: 183 VNRGLMYTLTFALGSGLAATAGALLGPIFVVTPTMGDLVALKAFAIVILGGLGNIPGATI 242

Query: 244 AAFIIGIVQEVSTPFLGSQYKQGVALLIMILVLLIRPKGLF 284
             F++ + +E    +L S Y+  +  L++I VL++RP+GLF
Sbjct: 243 GGFVLALAEEFGAGYLSSGYRDAMGFLLIIAVLIVRPQGLF 283


Lambda     K      H
   0.328    0.144    0.426 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 245
Number of extensions: 19
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 288
Length of database: 290
Length adjustment: 26
Effective length of query: 262
Effective length of database: 264
Effective search space:    69168
Effective search space used:    69168
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory