GapMind for catabolism of small carbon sources

 

Aligments for a candidate for mctC in Azospirillum brasilense Sp245

Align Monocarboxylic acid transporter (characterized)
to candidate AZOBR_RS02940 AZOBR_RS02940 actetate permease

Query= SwissProt::Q8NS49
         (551 letters)



>lcl|FitnessBrowser__azobra:AZOBR_RS02940 AZOBR_RS02940 actetate
           permease
          Length = 572

 Score =  439 bits (1130), Expect = e-127
 Identities = 247/556 (44%), Positives = 341/556 (61%), Gaps = 52/556 (9%)

Query: 23  ISVFVVFIIVTMTVVLRVGKSTSESTDFYTGGASFSGTQNGLAIAGDYLSAASFLGIVGA 82
           I +F+VF++ T+ +     + T  + DFY  G   +G QNGLAIAGDY+SAASFLGI G 
Sbjct: 42  IVMFLVFVLATLGITYWAARRTKSAKDFYAAGGGITGFQNGLAIAGDYMSAASFLGIAGL 101

Query: 83  ISLNGYDGFLYSIGFFVAWLVALLLVAEPLRNVGRFTMADVLSFRLRQKPVRVAAACGTL 142
           +  +G+DG ++S+G+ V W + L LVAE LRN+G++T ADV S+R +Q P+R  AACG+L
Sbjct: 102 VYTSGFDGLIFSVGWLVGWPIILFLVAERLRNLGKYTFADVASYRFQQTPMRTMAACGSL 161

Query: 143 AVTLFYLIAQMAGAGSLVSVLLDIHEFKWQAVVVGIVGIVMIAYVLLGGMKGTTYVQMIK 202
           A   FYLIAQM GAG L+ +L  + ++ W  V   IVG++MIAYV  GGM  TT+VQ+IK
Sbjct: 162 ATVTFYLIAQMVGAGKLIQLLFGM-DYLWAVV---IVGVLMIAYVTFGGMLATTWVQIIK 217

Query: 203 AVLLVGGVAIMTVLTFVKVSGGLTTLLNDAVEKHAASDYAATKGYDPTQILEPGLQYGAT 262
           AVLL+ G + M      K       + + AV+ H  +          T I+ P    GA 
Sbjct: 218 AVLLLSGASFMAFAVLAKFGFSPEAMFSTAVQVHPKA----------TGIMAP----GAL 263

Query: 263 LTTQLDFISLALALCLGTAGLPHVLMRFYTVPTAKEARKSVTWAIVLIGAFYLMTLVLGY 322
           +T  +  ISL +AL  GTAGLPH+LMRF+TV  AKEARKSV +A   IG FY++T ++G+
Sbjct: 264 ITDPVSAISLGMALMFGTAGLPHILMRFFTVSDAKEARKSVFYATGFIGYFYILTFIIGF 323

Query: 323 GA-AALVGPDRVIAAP--------------------GAANAAAPLLAFELGGSIFMALIS 361
           GA   L+ PD   A P                    G +N AA   A  +GG +F   IS
Sbjct: 324 GAIVLLLAPDATGAYPFLDAAKLAAAGGKPNPSMIIGGSNMAAIHTAHAVGGDLFFGFIS 383

Query: 362 AVAFATVLAVVAGLAITASAAVGHDIYNAVIRNGQSTEAEQVRVSRITVVVIGLISIVLG 421
           AVAFAT+LAVVAGL +  ++AV HD+Y +VI  G+++E +++RVS+IT V+IG++SI LG
Sbjct: 384 AVAFATILAVVAGLTLAGASAVSHDLYASVIAKGRASEHDEIRVSKITTVIIGIVSIFLG 443

Query: 422 ILAMTQNVAFLVALAFAVAASANLPTILYSLYWKKFNTTGAVAAIYTGLISALLLIFLSP 481
           I    QNVAF+V LAF +AASAN P +L S++W +  T GAV   + GL+S++ L+ + P
Sbjct: 444 IAFENQNVAFMVGLAFVIAASANFPVLLMSMFWSRMTTRGAVLGGWIGLVSSVSLLIMGP 503

Query: 482 AVSGNDSAMVPGADWAIFPLKNPGLVSIPLAFIAGWIGTLVGKPDNMDDLAAEME----- 536
            V  +    V G   A+FP  NPG+ +IPL+F+A W  ++    DN      E E     
Sbjct: 504 TVWKS----VLGNPAALFPYDNPGVFTIPLSFLAIWFFSIT---DNSKAAQDEREAYKAQ 556

Query: 537 -VRSLTGVGVEKAVDH 551
            +RS TG+G E A  H
Sbjct: 557 YIRSQTGLGAEGASAH 572


Lambda     K      H
   0.324    0.138    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 734
Number of extensions: 33
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 551
Length of database: 572
Length adjustment: 36
Effective length of query: 515
Effective length of database: 536
Effective search space:   276040
Effective search space used:   276040
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory