Align PTS system, fructose-specific, IIC component, component of D-allose/D-ribose transporting Enzyme II complex (Fru2; IIA/IIB/IIC) (Patron et al. 2017). This system is similar to Frz of E. coli (TC#4.A.2.1.9) which is involved in environmental sensing, host adaptation and virulence (characterized)
to candidate AZOBR_RS32335 AZOBR_RS32335 PTS fructose transporter subunit IIBC
Query= TCDB::Q3JZE4 (367 letters) >FitnessBrowser__azobra:AZOBR_RS32335 Length = 580 Score = 203 bits (517), Expect = 8e-57 Identities = 123/339 (36%), Positives = 188/339 (55%), Gaps = 17/339 (5%) Query: 13 HLLTAISYLIPIVCGAGFLVAIGMAMGG----RAQDALVIGNFSFWDTMATMGGK-ALGL 67 HL+T +S+++P V G L+AI A+GG +GN F +G K A L Sbjct: 245 HLMTGVSFMLPFVVTGGLLIAIAFALGGIYVFEDSQQGTLGNALF-----QIGAKGAFAL 299 Query: 68 LPVIIATGIAFSIAGKPGIAPGFVVGLIANAISAGFIGGILGGYIAGFIALGILKNVKVP 127 + +A IAFSIA +PGI PG V G++A + AGF+GGI+ G+IAG+ + +N+++ Sbjct: 300 MVPALAGYIAFSIADRPGITPGMVGGILAANLGAGFLGGIVAGFIAGYATSFLNRNIRLH 359 Query: 128 NWAKGLMPTLIVPFFAALLSSLIMIYLIGGPISAFTGWLTDFLKSLGNTSNLVMGAVIGV 187 +GL P LI+P +L++ L MIY++G P++ L+ +LK + +S +++G +IG Sbjct: 360 KNLEGLKPVLILPLLGSLVTGLAMIYVVGAPVAEALATLSAWLKGMQGSSAILLGLLIGA 419 Query: 188 LSGVDLGGPLNKTVYAFVLTLQAEGVKEPLTALQLVNTATPVGFGLAYFIAKLFKKNIYT 247 + D+GGP+NK YAF L A V P+ A P+G LA KLF +T Sbjct: 420 MMAFDMGGPVNKAAYAFSTGLIASQVYTPMAAAMAAGMVPPLGLALA---TKLFADR-FT 475 Query: 248 NDEIETLKSAVPMGIVNIVEGVIPIVMNNLMPAILAIGVGGAVGGAVSMTMGADSAVPFG 307 +E E +A +GI I EG IP + + I A+ +G A+ GA+SM +GA+ VP G Sbjct: 476 REEREAGNAAGILGIAFITEGAIPFAARDPLRVIPALVLGAALTGAISMGIGAELKVPHG 535 Query: 308 GI--LMIPTMTRPIAG-ICGLLSNILVTGLVYSLAKKPV 343 GI L IP +AG + L++ + T + K+PV Sbjct: 536 GIFVLPIPNAVTHLAGYVVALVAGTVTTAVALRFLKRPV 574 Lambda K H 0.323 0.143 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 671 Number of extensions: 47 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 367 Length of database: 580 Length adjustment: 33 Effective length of query: 334 Effective length of database: 547 Effective search space: 182698 Effective search space used: 182698 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory