Alignments for a candidate for Dshi_1194 in Azospirillum brasilense Sp245

GapMind for catabolism of small carbon sources

Alignments for a candidate for Dshi_1194 in Azospirillum brasilense Sp245

Align TRAP transporter, 4TM/12TM fusion protein (characterized, see rationale)
to candidate AZOBR_RS16500 AZOBR_RS16500 C4-dicarboxylate ABC transporter permease

Query= uniprot:E4PQE4
         (729 letters)



>FitnessBrowser__azobra:AZOBR_RS16500
          Length = 673

 Score =  383 bits (983), Expect = e-110
 Identities = 213/533 (39%), Positives = 309/533 (57%), Gaps = 26/533 (4%)

Query: 219 LIMELTRRVAGMALVIIGLVFLAYVFAGPYLPGFLGYPGLSVQRFFSQVY-TDAGILGPT 277
           +++E TRR  G  L II L+F++Y   GP +PG L +PG +V +    +Y T  G+ G  
Sbjct: 141 VLLEATRRSVGWPLPIISLIFMSYAIWGPQMPGLLKHPGATVSQLVDHLYLTTQGVYGIA 200

Query: 278 TAVSSTYIILFIIFAAFLQSSKVGDYFVNFAFAAAGRSRGGPAKVSIFASGLMGMINGTS 337
             V +TY+  F++F  F     +G  F++ A   AGR  GGPAKV+IF S L GMI+G+S
Sbjct: 201 LGVVATYVFHFVLFGVFATRIGLGQLFLDCAAWVAGRYAGGPAKVAIFGSALFGMISGSS 260

Query: 338 AGNVVSTGSLTIPLMKKVGYSKQSAGAVEAAASTGGQIMPPIMGAGAFIMAEITGIPYTE 397
             N V+ GSLTIP MK++GY    A AVE+ ASTGGQI PPIMGA AF+M E  G+PYT 
Sbjct: 261 VANTVTVGSLTIPAMKRMGYKPHFAAAVESTASTGGQITPPIMGAAAFLMIEFLGLPYTT 320

Query: 398 IAIAAIIPAILYFASVYFMVDFEAAKTGMRGMREDELPKLRTMMKQCY-LFVPIIILIVA 456
           I +AAI+PA ++F  V   V FEA + G+RG+R DE+P L+   ++ +   +P+++LI  
Sbjct: 321 IIMAAIVPAFMHFFGVLVQVHFEAKRNGLRGLRPDEMPDLKEAFRRDWPTVIPLVVLIGI 380

Query: 457 LFMGYSVIRAGTLATVSAAVVSWLSPNK-MGLRHILQALEIASYMAIQIIVVCAAAGVIV 515
           L  GY+   A          V  L+P K M +  +L  L   +  A+ +    A  G+IV
Sbjct: 381 LIAGYTPYMAAFWGITLCIAVGLLNPRKRMTIWEVLDGLRDGAKYALAVGAAAATVGIIV 440

Query: 516 GVISLTGVGARFSVL-------------------LLDVAATSQLLALIFAMFISILLGMG 556
           GV++LTGVG + S +                   L D  A + L  LI    + IL+G G
Sbjct: 441 GVVTLTGVGFKISYIVTSTAGEMAVWAGAFLPGWLADAKALTLLFTLIMTGVVCILMGCG 500

Query: 557 MPTTAAYAVAASVVAPGLVQLGIEPLTAHFFVFYFAVVSAITPPVALASYAAAGISGANA 616
           +PTTA Y + A++ AP L  LG+ P+ AHFFVFY+ V++ ITPPVALA+YAAAG++GA+ 
Sbjct: 501 IPTTANYIIMATIAAPALGLLGVAPIVAHFFVFYYGVLADITPPVALAAYAAAGMAGADP 560

Query: 617 METSVASFRIGIAAFIVPFMFFYNGALLMEA---GWFEIARALVTATFGVYMLSGGVLGW 673
            +T   +FR+G+A  +VPF+F ++ +LL+ A    W +   A +    G+  L   + GW
Sbjct: 561 FKTGNTAFRLGLAKALVPFVFVFSPSLLLVAPGFNWPDFFIAFLGCIVGIVCLGATLTGW 620

Query: 674 FASISASWITRLLLIAAALLMIEGGLWTDLTGIALAVLAFVIQKQRKTRLATA 726
             +    W  RLLL  AA+L++   L++ L G+AL V   + Q   +  +  A
Sbjct: 621 LLTNMRGW-ERLLLGIAAILLVTPELYSSLLGLALIVPVLLRQMSARRLMPEA 672



 Score = 26.9 bits (58), Expect = 0.003
 Identities = 15/44 (34%), Positives = 21/44 (47%)

Query: 43 KFVALLAIAYSAFHLYSLNIAPLETWSFRIVHIAGALVLGFVLF 86
          K V  L I  S FH Y+     L     R +H++  L L F++F
Sbjct: 30 KLVGGLLIILSLFHYYTAGFGLLPEMEHRGIHLSFVLGLVFLVF 73


Lambda     K      H
   0.327    0.139    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1273
Number of extensions: 83
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 729
Length of database: 673
Length adjustment: 39
Effective length of query: 690
Effective length of database: 634
Effective search space:   437460
Effective search space used:   437460
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources