Alignments for a candidate for fruK in Azospirillum brasilense Sp245

GapMind for catabolism of small carbon sources

Alignments for a candidate for fruK in Azospirillum brasilense Sp245

Align Fructose import ATP-binding protein FruK; EC 7.5.2.- (characterized)
to candidate AZOBR_RS31880 AZOBR_RS31880 sugar ABC transporter ATP-binding protein

Query= SwissProt::Q8G847
         (513 letters)



>FitnessBrowser__azobra:AZOBR_RS31880
          Length = 515

 Score =  261 bits (667), Expect = 4e-74
 Identities = 162/490 (33%), Positives = 272/490 (55%), Gaps = 28/490 (5%)

Query: 1   MTDKNPIVVMKGITIEFPGVKALDGVDLTLYPGEVHALMGENGAGKSTMIKALTGVYKIN 60
           MT    ++ + GIT  F  + A D + LTL+ GEV AL+GENGAGK+T++  L G Y  +
Sbjct: 1   MTSPPIVLRLSGITKRFGPLVANDSISLTLHKGEVLALLGENGAGKTTLMNILFGHYVAD 60

Query: 61  AGSIMVDGKPQQFNGTLDAQNAGIATVYQEVNLCTNLSVGENVMLGHEKRGPFGIDWKKT 120
            GSI   G+P        A  AGI  V+Q   L  NLSV +N+ +G E        W+  
Sbjct: 61  EGSIEAFGQPLPPGSPRAALAAGIGMVHQHFTLADNLSVLDNIAVGTESL------WRPR 114

Query: 121 HE--AAKKYLAQM----GLESIDPHTPLSSISIAMQQLVAIARAMVINAKVLILDEPTSS 174
            +  AAK  L  +    GLE + P   +  +S+  +Q   I +A+  +A+VLILDEPT+ 
Sbjct: 115 SDRAAAKAKLLDLARRFGLE-VRPDALVGDLSVGERQRAEILKALYRDARVLILDEPTAV 173

Query: 175 LDANEVRDLFAIMRKVRDSGVAILFVSHFLDQIYEITDRLTILRNGQFIKEVMTKDTPRD 234
           L   E   LF  +R++   G+A++F+SH +++++  +D + +LR G+ +    T +T R+
Sbjct: 174 LTPQESASLFDTLRRLTADGLAVVFISHKMNEVFAASDTVAVLRGGRLVATRKTAETDRE 233

Query: 235 ELIGMMIGKSAAELSQIGAKKARREITPGEKPIVDVKGLGK-----KGTINPVDVDIYKG 289
           EL  +M+G++    +          + PGE P++ + G+       +  ++ VD+ + + 
Sbjct: 234 ELAELMVGRALKPPTPT-------PLEPGE-PVLTLSGVTVASGHGRPLLDGVDLTVRRR 285

Query: 290 EVVGFAGLLGSGRTELGRLLYGADKPDSGTYTLNGKKVNISDPYTALKNKIAYSTENRRD 349
           ++VG AG+ G+G+T L  L+ G   PDSGT TL G+ +  ++P   ++  +A   E+R  
Sbjct: 286 QIVGIAGVSGNGQTALAELISGLIHPDSGTMTLKGEAIGNAEPAEMVRRGVARIPEDRHA 345

Query: 350 EGIIGDLTVRQNILIALQATRGM--FKPIPKKEADAIVDKYMKELNVRPADPDRPVKNLS 407
            G++G + V +N++           F  I +  A A  ++ +   +VR   PD   + LS
Sbjct: 346 AGLVGAMAVWENLIAERYHDPAFQRFGLIRRGAARAYAEEVIAAFDVRCPGPDARTQLLS 405

Query: 408 GGNQQKVLIGRWLATHPELLILDEPTRGIDIGAKAEIQQVVLDLASQGMGVVFISSELEE 467
           GGN QK+++GR LA  P+L++  +PTRG+D+GA + +   +L+  + G GV+ IS +L+E
Sbjct: 406 GGNMQKLILGRTLAHGPDLILASQPTRGLDVGAVSYVHGRLLEARAAGAGVLVISEDLDE 465

Query: 468 VVRLSDDIEV 477
           ++ L+D I V
Sbjct: 466 ILALADGITV 475



 Score = 71.6 bits (174), Expect = 6e-17
 Identities = 61/214 (28%), Positives = 103/214 (48%), Gaps = 12/214 (5%)

Query: 282 VDVDIYKGEVVGFAGLLGSGRTELGRLLYGADKPDSGTYTLNGKKVNISDPYTALKNKIA 341
           + + ++KGEV+   G  G+G+T L  +L+G    D G+    G+ +    P  AL   I 
Sbjct: 26  ISLTLHKGEVLALLGENGAGKTTLMNILFGHYVADEGSIEAFGQPLPPGSPRAALAAGIG 85

Query: 342 YSTENRRDEGIIGDLTVRQNILIALQATRGMFKPIPKKEADA--IVDKYMK-ELNVRPAD 398
              ++     +  +L+V  NI +    T  +++P   + A    ++D   +  L VRP  
Sbjct: 86  MVHQHFT---LADNLSVLDNIAVG---TESLWRPRSDRAAAKAKLLDLARRFGLEVRP-- 137

Query: 399 PDRPVKNLSGGNQQKVLIGRWLATHPELLILDEPTRGIDIGAKAEIQQVVLDLASQGMGV 458
            D  V +LS G +Q+  I + L     +LILDEPT  +     A +   +  L + G+ V
Sbjct: 138 -DALVGDLSVGERQRAEILKALYRDARVLILDEPTAVLTPQESASLFDTLRRLTADGLAV 196

Query: 459 VFISSELEEVVRLSDDIEVLKDRHKIAEIENDDT 492
           VFIS ++ EV   SD + VL+    +A  +  +T
Sbjct: 197 VFISHKMNEVFAASDTVAVLRGGRLVATRKTAET 230



 Score = 65.1 bits (157), Expect = 6e-15
 Identities = 48/225 (21%), Positives = 106/225 (47%), Gaps = 10/225 (4%)

Query: 6   PIVVMKGITIEFP-GVKALDGVDLTLYPGEVHALMGENGAGKSTMIKALTGVYKINAGSI 64
           P++ + G+T+    G   LDGVDLT+   ++  + G +G G++ + + ++G+   ++G++
Sbjct: 257 PVLTLSGVTVASGHGRPLLDGVDLTVRRRQIVGIAGVSGNGQTALAELISGLIHPDSGTM 316

Query: 65  MVDGKPQQFNGTLDAQNAGIATVYQE---VNLCTNLSVGENVMLGHEKRGP----FGIDW 117
            + G+        +    G+A + ++     L   ++V EN ++      P    FG+  
Sbjct: 317 TLKGEAIGNAEPAEMVRRGVARIPEDRHAAGLVGAMAVWEN-LIAERYHDPAFQRFGLIR 375

Query: 118 KKTHEA-AKKYLAQMGLESIDPHTPLSSISIAMQQLVAIARAMVINAKVLILDEPTSSLD 176
           +    A A++ +A   +    P      +S    Q + + R +     +++  +PT  LD
Sbjct: 376 RGAARAYAEEVIAAFDVRCPGPDARTQLLSGGNMQKLILGRTLAHGPDLILASQPTRGLD 435

Query: 177 ANEVRDLFAIMRKVRDSGVAILFVSHFLDQIYEITDRLTILRNGQ 221
              V  +   + + R +G  +L +S  LD+I  + D +T+  +G+
Sbjct: 436 VGAVSYVHGRLLEARAAGAGVLVISEDLDEILALADGITVAYHGR 480


Lambda     K      H
   0.316    0.135    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 638
Number of extensions: 32
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 3
Length of query: 513
Length of database: 515
Length adjustment: 35
Effective length of query: 478
Effective length of database: 480
Effective search space:   229440
Effective search space used:   229440
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources