Alignments for a candidate for galU in Azospirillum brasilense Sp245

GapMind for catabolism of small carbon sources

Alignments for a candidate for galU in Azospirillum brasilense Sp245

Align UTP--glucose-1-phosphate uridylyltransferase; Alpha-D-glucosyl-1-phosphate uridylyltransferase; General stress protein 33; GSP33; UDP-glucose pyrophosphorylase; UDPGP; Uridine diphosphoglucose pyrophosphorylase; EC 2.7.7.9 (characterized)
to candidate AZOBR_RS31075 AZOBR_RS31075 UTP--glucose-1-phosphate uridylyltransferase

Query= SwissProt::Q05852
         (292 letters)



>FitnessBrowser__azobra:AZOBR_RS31075
          Length = 289

 Score =  296 bits (758), Expect = 4e-85
 Identities = 151/280 (53%), Positives = 195/280 (69%), Gaps = 3/280 (1%)

Query: 2   KKVRKAIIPAAGLGTRFLPATKAMPKEMLPIVDKPTIQYIIEEAVEAGIEDIIIVTGKSK 61
           K VRK + P AGLGTRFLPATKA+PKEMLP+VD+P +Q+ +EEA  AGIED + VTG+SK
Sbjct: 3   KPVRKVVFPVAGLGTRFLPATKAIPKEMLPLVDRPLLQHAVEEARAAGIEDFVFVTGRSK 62

Query: 62  RAIEDHFDYSPELERNLEEKGKTELLEKVKKASNLAD--IHYIRQKEPKGLGHAVWCARN 119
           RAIEDHFD   EL R LEE+GK + LE V+  S +A     Y RQ+ P GLGHAVWCAR 
Sbjct: 63  RAIEDHFDTDTELNRTLEERGKQDALEVVRD-SEIAPGRCFYTRQQVPLGLGHAVWCARA 121

Query: 120 FIGDEPFAVLLGDDIVQAETPGLRQLMDEYEKTLSSIIGVQQVPEEETHRYGIIDPLTSE 179
            IG++PFA++L DD VQ +TP L+Q+++ Y +   +I+ V  VP E T  YGI+D    +
Sbjct: 122 VIGNDPFAIVLPDDFVQGDTPCLKQMVEAYNEVGGNIVAVVDVPRERTSSYGILDVEKDD 181

Query: 180 GRRYQVKNFVEKPPKGTAPSNLAILGRYVFTPEIFMYLEEQQVGAGGEIQLTDAIQKLNE 239
           GR   V+  VEKP    APS L+I+GRY+  PE+F +LE+QQ GAG EIQLTDA+ KL  
Sbjct: 182 GRLATVRGLVEKPKPEEAPSTLSIIGRYILQPEVFDHLEKQQRGAGNEIQLTDAMAKLIG 241

Query: 240 IQRVFAYDFEGKRYDVGEKLGFITTTLEFAMQDKELRDQL 279
            Q      FEG RYD G+K+GFI  TL  A++  ++ D++
Sbjct: 242 TQPFHGLRFEGTRYDCGDKVGFIEATLAHALRRPDMADKV 281


Lambda     K      H
   0.317    0.138    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 289
Number of extensions: 8
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 292
Length of database: 289
Length adjustment: 26
Effective length of query: 266
Effective length of database: 263
Effective search space:    69958
Effective search space used:    69958
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 48 (23.1 bits)

Align candidate AZOBR_RS31075 AZOBR_RS31075 (UTP--glucose-1-phosphate uridylyltransferase)
to HMM TIGR01099 (galU: UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01099.hmm
# target sequence database:        /tmp/gapView.28732.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01099  [M=261]
Accession:   TIGR01099
Description: galU: UTP--glucose-1-phosphate uridylyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   2.2e-109  351.0   0.0   2.6e-109  350.8   0.0    1.0  1  lcl|FitnessBrowser__azobra:AZOBR_RS31075  AZOBR_RS31075 UTP--glucose-1-pho


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__azobra:AZOBR_RS31075  AZOBR_RS31075 UTP--glucose-1-phosphate uridylyltransferase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  350.8   0.0  2.6e-109  2.6e-109       1     261 []       5     266 ..       5     266 .. 0.98

  Alignments for each domain:
  == domain 1  score: 350.8 bits;  conditional E-value: 2.6e-109
                                 TIGR01099   1 irkaviPaaGlGtrlLPatkaiPkemlpivdkPliqyvveeaveaGieeivlvtgrskraiedhfDtsy 69 
                                               +rk+v+P+aGlGtr+LPatkaiPkemlp+vd+Pl+q++veea +aGie+ v+vtgrskraiedhfDt++
  lcl|FitnessBrowser__azobra:AZOBR_RS31075   5 VRKVVFPVAGLGTRFLPATKAIPKEMLPLVDRPLLQHAVEEARAAGIEDFVFVTGRSKRAIEDHFDTDT 73 
                                               79******************************************************************* PP

                                 TIGR01099  70 eleaklekknkeellkevrkiael.atilyvrqkeakGLGhavllaeelvgdepfavllgDdlvseeee 137
                                               el+++le+++k+++l+ vr+      +++y+rq+   GLGhav++a++++g++pfa++l+Dd v+ +++
  lcl|FitnessBrowser__azobra:AZOBR_RS31075  74 ELNRTLEERGKQDALEVVRDSEIApGRCFYTRQQVPLGLGHAVWCARAVIGNDPFAIVLPDDFVQGDTP 142
                                               ******************98654449******************************************* PP

                                 TIGR01099 138 alkqlielyektgasiiaveevpkeevskYGvidgeeveeelyevkdlvekPkpeeapsnlaivGrYvl 206
                                               +lkq++e+y+++g +i+av +vp+e +s+YG++d+e+ + +l +v+ lvekPkpeeaps l i+GrY+l
  lcl|FitnessBrowser__azobra:AZOBR_RS31075 143 CLKQMVEAYNEVGGNIVAVVDVPRERTSSYGILDVEKDDGRLATVRGLVEKPKPEEAPSTLSIIGRYIL 211
                                               ********************************************************************* PP

                                 TIGR01099 207 tpeifelleetkaGkggeiqltDalrlllekeevlavklkgkryDvGdklgylka 261
                                               +pe+f++le++++G+g+eiqltDa+++l+ ++ +++ +++g+ryD+Gdk+g+++a
  lcl|FitnessBrowser__azobra:AZOBR_RS31075 212 QPEVFDHLEKQQRGAGNEIQLTDAMAKLIGTQPFHGLRFEGTRYDCGDKVGFIEA 266
                                               *****************************************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (261 nodes)
Target sequences:                          1  (289 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 11.25
//
[ok]

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources