GapMind for catabolism of small carbon sources

 

Alignments for a candidate for gloB in Azospirillum brasilense Sp245

Align Hydroxyacylglutathione hydrolase GloC; Accessory type II glyoxalase; Glyoxalase II 2; GlxII-2; EC 3.1.2.6 (characterized)
to candidate AZOBR_RS19250 AZOBR_RS19250 hypothetical protein

Query= SwissProt::P75849
         (215 letters)



>FitnessBrowser__azobra:AZOBR_RS19250
          Length = 210

 Score =  278 bits (711), Expect = 5e-80
 Identities = 119/206 (57%), Positives = 155/206 (75%)

Query: 5   IIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDDSGLTLMQILLTHGHLDHVGA 64
           I+PVT F QNC+++WC +T   A VDPGGD  ++ +     G+TL +IL+THGH+DH GA
Sbjct: 5   IVPVTPFQQNCTVLWCPETMKGAAVDPGGDLPRVLRAAQSKGVTLEKILVTHGHIDHAGA 64

Query: 65  AAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEECQPLTPDRWLNEGDTISIGNVTL 124
            A+LA    +P+ GP +ED+FW+ G+P QS+MFG    +  TPDRWL EGDT+++GN+TL
Sbjct: 65  VADLADELKLPIEGPHREDQFWIDGMPMQSQMFGFPPVRSFTPDRWLEEGDTVTVGNLTL 124

Query: 125 QVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGRSDFPRGDHNQLISSIKDKLLPLGD 184
            V HCPGHTPGHVVF    +K+ I GDV+F+G +GR+DFP+G+H  LI SI+ KL PLGD
Sbjct: 125 DVHHCPGHTPGHVVFVHKPSKIAIVGDVLFQGSIGRTDFPKGNHGDLIESIRSKLFPLGD 184

Query: 185 DVIFIPGHGPLSTLGYERLHNPFLQD 210
           DV FIPGHGP ST+G ERL+NPFL D
Sbjct: 185 DVTFIPGHGPTSTIGEERLYNPFLND 210


Lambda     K      H
   0.321    0.142    0.453 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 265
Number of extensions: 13
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 215
Length of database: 210
Length adjustment: 21
Effective length of query: 194
Effective length of database: 189
Effective search space:    36666
Effective search space used:    36666
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 45 (21.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory