GapMind for catabolism of small carbon sources

 

Aligments for a candidate for lldE in Azospirillum brasilense Sp245

Align Uncharacterized protein (characterized, see rationale)
to candidate AZOBR_RS01615 AZOBR_RS01615 Fe-S oxidoreductase

Query= uniprot:B2TBW0
         (256 letters)



>lcl|FitnessBrowser__azobra:AZOBR_RS01615 AZOBR_RS01615 Fe-S
           oxidoreductase
          Length = 261

 Score =  156 bits (395), Expect = 4e-43
 Identities = 91/248 (36%), Positives = 130/248 (52%), Gaps = 16/248 (6%)

Query: 11  KVALFIPCFIDAFYPEVGIATLELLERFGIQVDYPQEQTCCGQPMANSGAHAEAAGTERV 70
           +V LF+ C +D + P VG A ++LLE  G  V+ P  QTCCGQP  N+G  A A    R 
Sbjct: 9   RVGLFVTCLVDVYRPTVGFAAVKLLEDAGCTVEVPASQTCCGQPAFNAGDRATARDLARS 68

Query: 71  FARNFAGYDYIVGPSASCIHHVREHLTALEQTD-----EVKKVRANAYELVEFLHDVVGA 125
               F G+D++V PS SC   +R+H   L   D       + + A  +ELV FL DV+G 
Sbjct: 69  TIAAFEGFDHVVVPSGSCAGQIRKHYPELLADDPAWAARARHLSARTHELVSFLTDVLGV 128

Query: 126 REFPWAEFPHRVGLHNSCSALRHLKEASISEVAGAPFSKPRTLLEGVKGIEFVKPARPDE 185
            +   A +   +  H+SCS LR   E  + E       +PR LL  V+G+   +    + 
Sbjct: 129 TD-TGARYDGTITYHDSCSGLR---ELGVKE-------QPRRLLAKVEGLTLNELPGAEV 177

Query: 186 CCGFGGTFSVTEEPVSVRMGQDKVRDHLNAGAEYIVSGDMSCLMHQQGCAERMKADARFI 245
           CCGFGGTF V    +S RM + K  D     A+ +++GDM CL++  G  +R  +  R  
Sbjct: 178 CCGFGGTFCVKYPDISNRMVEAKTADIAGTKADTLLAGDMGCLLNMAGKLKREGSAVRVR 237

Query: 246 HIAQVLNG 253
           H+A+VL G
Sbjct: 238 HVAEVLAG 245


Lambda     K      H
   0.323    0.137    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 210
Number of extensions: 13
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 256
Length of database: 261
Length adjustment: 24
Effective length of query: 232
Effective length of database: 237
Effective search space:    54984
Effective search space used:    54984
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory