GapMind for catabolism of small carbon sources

 

Alignments for a candidate for pccA1 in Azospirillum brasilense Sp245

Align acyl CoA carboxylase biotin carboxylase subunit (EC 2.1.3.15; EC 6.4.1.3; EC 6.3.4.14) (characterized)
to candidate AZOBR_RS12015 AZOBR_RS12015 acetyl-CoA carboxylase biotin carboxylase subunit

Query= metacyc::MONOMER-13597
         (509 letters)



>FitnessBrowser__azobra:AZOBR_RS12015
          Length = 447

 Score =  397 bits (1019), Expect = e-115
 Identities = 210/445 (47%), Positives = 292/445 (65%), Gaps = 5/445 (1%)

Query: 4   FSRVLVANRGEIATRVLKAIKEMGMTAIAVYSEADKYAVHTKYADEAYYIGKAPALDSYL 63
           F +VL+ANRGEIA R+ +A +EMG+  +AV+S AD  A+H + ADE+  IG A A +SYL
Sbjct: 2   FEKVLIANRGEIALRIHRACREMGIQTVAVHSTADADAMHVRLADESVCIGPASARESYL 61

Query: 64  NIEHIIDAAEKAHVDAIHPGYGFLSENAEFAEAVEKAGITFIGPSSEVMRKIKDKLDGKR 123
           NI  I+ AA     DAIHPG GFLSENA+FAE VE+ G TFIGP++E +R + DK+  K+
Sbjct: 62  NIPAILSAASITGADAIHPGIGFLSENAQFAEMVEEHGFTFIGPTAEHIRIMGDKVTAKK 121

Query: 124 LANMAGVPTAPGSDGPVTSIDEALKLAEKIGYPIMVKAASGGGGVGITRVDNQDQLMDVW 183
                G+P  PGSDGPV +++EA K+  + GYPI++KAA+GGGG G+    N DQL + +
Sbjct: 122 TVMEQGLPVVPGSDGPVPTLEEAEKIGRETGYPILIKAAAGGGGKGMKVARNPDQLKEAY 181

Query: 184 ERNKRLAYQAFGKADLFIEKYAVNPRHIEFQLIGDKYGNYVVAWERECTIQRRNQKLIEE 243
           +  +  A  AFG  +++IEKY   PRHIE QL+GD++GN V   ER+C++QRR+QK+IEE
Sbjct: 182 QLARGEARAAFGNDEVYIEKYLGKPRHIEIQLLGDEHGNCVHFGERDCSVQRRHQKVIEE 241

Query: 244 APSPALKMEERESMFEPIIKFGKLINYFTLGTFETAFSDVSRDFYFLELNKRLQVEHPTT 303
           APSPAL  E+R  + +   K    I Y  +GT E  F D    FYF+E+N RLQVEH  T
Sbjct: 242 APSPALNAEQRAFIGDLAAKTAAAIGYRGVGTMEFLFED--GQFYFIEMNTRLQVEHTIT 299

Query: 304 ELIFRIDLVKLQIKLAAGEHLPFSQEDLNKRVRGTAIEYRINAEDALNNFTGSSGFVTYY 363
           E+I  IDLV+ QI++A G  L + Q D+  R +G +IE R+NAE     F  S G +  Y
Sbjct: 300 EMITGIDLVREQIRVATGAPLGYGQADI--RFQGHSIECRVNAEHP-ETFIPSPGKIDGY 356

Query: 364 REPTGPGVRVDSGIESGSYVPPYYDSLVSKLIVYGESREYAIQAGIRALADYKIGGIKTT 423
             P G GVRVDS +  G  +PP+YDSL++KL+V+G +R   +    R + +Y IGG+ TT
Sbjct: 357 HAPGGLGVRVDSALYDGYRIPPHYDSLIAKLVVHGTTRNECLMRLRRTIEEYVIGGVDTT 416

Query: 424 IELYKWIMQDPDFQEGKFSTSYISQ 448
           + L++ I+    F +G +   ++ Q
Sbjct: 417 LPLHQRIIAQQAFIDGNYDIHWLEQ 441


Lambda     K      H
   0.317    0.135    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 581
Number of extensions: 19
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 509
Length of database: 447
Length adjustment: 33
Effective length of query: 476
Effective length of database: 414
Effective search space:   197064
Effective search space used:   197064
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory