Aligments for a candidate for pccB in Azospirillum brasilense Sp245

GapMind for catabolism of small carbon sources

Aligments for a candidate for pccB in Azospirillum brasilense Sp245

Align Propionyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.3) (characterized)
to candidate AZOBR_RS21100 AZOBR_RS21100 methylmalonyl-CoA carboxyltransferase

Query= reanno::PS:Dsui_0517
         (510 letters)



>lcl|FitnessBrowser__azobra:AZOBR_RS21100 AZOBR_RS21100
           methylmalonyl-CoA carboxyltransferase
          Length = 510

 Score =  821 bits (2121), Expect = 0.0
 Identities = 400/510 (78%), Positives = 452/510 (88%)

Query: 1   MHDIIHELEKKREAARLGGGQKRIDSQHKKGKLTARERLELLLDPDSFEEWDMFKEHRCT 60
           M +I+ +L+  R  ARLGGG+KR+  QH KGKLTARER+EL LD  SFEE+DMF +HRC 
Sbjct: 1   MQEILAKLDAMRSGARLGGGEKRVAGQHAKGKLTARERIELFLDEGSFEEFDMFVQHRCN 60

Query: 61  DFGMAETKNPGDGVVTGYGTINGRLVFVFSQDFTVFGGSLSETHAEKICKVMDHAMKVGA 120
           DFGM   K PGDGVVTG+GT+NGRLVFVFSQDFTVFGGSLSE HAEKICK+MD AMKVGA
Sbjct: 61  DFGMEGQKVPGDGVVTGHGTVNGRLVFVFSQDFTVFGGSLSEAHAEKICKIMDQAMKVGA 120

Query: 121 PVIGLNDSGGARIQEGVASLGGYADVFQRNVMASGVIPQISMIMGPCAGGAVYSPAMTDF 180
           PVIGLNDSGGARIQEGVASLGGYA+VFQRNV ASGVIPQIS+IMGPCAGGAVYSPAMTDF
Sbjct: 121 PVIGLNDSGGARIQEGVASLGGYAEVFQRNVNASGVIPQISLIMGPCAGGAVYSPAMTDF 180

Query: 181 IFMVKDSSYMFVTGPEVVKTVTHEEVTAEELGGAVTHTTKSGVADLAFENDVEALNYLRR 240
           IFMVKDSSYMFVTGP+VVKTVTHE VTAEELGGA+TH++KSGVAD+AFENDVEAL  LRR
Sbjct: 181 IFMVKDSSYMFVTGPDVVKTVTHEVVTAEELGGAITHSSKSGVADMAFENDVEALLQLRR 240

Query: 241 LVNFLPANNREKPPVQKTNDPAERLDFSLDTLVPDNANKPYDMKELIIKMVDDCDFFEIQ 300
            ++FLPA+NRE+ P + T DP +R D SLDTLVP+N NKPYDMKELI+K VD+ DFFE+Q
Sbjct: 241 FIDFLPASNRERAPERPTADPIDRDDLSLDTLVPENPNKPYDMKELILKTVDEGDFFELQ 300

Query: 301 PDYAKNIITGFARMDGHPVGIVANQPLVLAGCLDIKSSIKAARFVRFCDAFNIPVVTLVD 360
           PDYAKNII GF RM+G  VGIVANQP+VLAGCLDI SS KAARFVRFCDAF IP++TLVD
Sbjct: 301 PDYAKNIIIGFGRMNGGTVGIVANQPMVLAGCLDIDSSKKAARFVRFCDAFEIPILTLVD 360

Query: 361 VPGFMPGTSQEYGGIIKHGAKLLYAYAECTVPKVTLITRKAYGGAYDVMSSKHLRGDVNL 420
           VPGFMPGTSQEYGGIIKHGAKLL+AYAE TVPK+T+ITRKAYGGAYDVM+SKHLRGD+N 
Sbjct: 361 VPGFMPGTSQEYGGIIKHGAKLLFAYAEATVPKITVITRKAYGGAYDVMASKHLRGDINY 420

Query: 421 AWPSAEIAVMGPKGAVEIIFREEKNDPAKLAEREAEYKAKFANPFVAGARGFIDDVIMPN 480
           AWPSAEIAVMGPKGAVEIIFR +  D AK+  R  EY+ KFANPFVA +RG+IDDVIMP+
Sbjct: 421 AWPSAEIAVMGPKGAVEIIFRGDIGDTAKIEARTEEYRQKFANPFVAASRGYIDDVIMPH 480

Query: 481 ETRKRICRSLAMLRDKKLDNPWRKHGNIPL 510
            TR+R+ ++L+ML++K+L NPWRKH NIPL
Sbjct: 481 GTRRRVIKALSMLKNKQLQNPWRKHDNIPL 510


Lambda     K      H
   0.320    0.137    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 914
Number of extensions: 31
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 510
Length of database: 510
Length adjustment: 34
Effective length of query: 476
Effective length of database: 476
Effective search space:   226576
Effective search space used:   226576
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources