GapMind for catabolism of small carbon sources

 

Aligments for a candidate for prpF in Azospirillum brasilense Sp245

Align Aconitate-delta-isomerase 1; Itaconic acid/2-hydroxyparaconate biosynthesis cluster protein ADI1; EC 5.-.-.- (characterized)
to candidate AZOBR_RS28105 AZOBR_RS28105 hypothetical protein

Query= SwissProt::A0A0U2X0E4
         (443 letters)



>lcl|FitnessBrowser__azobra:AZOBR_RS28105 AZOBR_RS28105 hypothetical
           protein
          Length = 374

 Score =  266 bits (680), Expect = 8e-76
 Identities = 161/354 (45%), Positives = 215/354 (60%), Gaps = 21/354 (5%)

Query: 5   IDTTIYRAGTSRGLYFLASDLPAEPSERDAALISIMGSGHPLQIDGMGGGNSLTSKVAIV 64
           I   + R GTSRG +FLASDLPA P+ERDA L+S+MG+G+ L IDG+GGGN LTSKVAIV
Sbjct: 11  IPCVMMRGGTSRGPFFLASDLPASPAERDALLLSVMGAGNDLGIDGIGGGNPLTSKVAIV 70

Query: 65  SASTQRSEFDVDYLFCQVGITERFVDTAPNCGNLMSGVAAFAIERGLVQPHPSDTTCLVR 124
             +T     DVDYLF QV + E  VDT+PNCGN+++ VA FAIE GLV    +D   +VR
Sbjct: 71  GPATLAGA-DVDYLFAQVRVQEGIVDTSPNCGNMLAAVAPFAIEAGLVPA--TDGVTVVR 127

Query: 125 IFNLNSRQASELVIPVYNGRVHYDD---IDDMHMQRPSARVGLRFLDTVGSCTGKLLPTG 181
           I N+N+ +  E  +   +GRV Y+    ID +     +A + L FLD  G+ TG+LLPTG
Sbjct: 128 IHNVNTGKLIEARVQTPDGRVTYEGEAVIDGV--PGTAAPIHLAFLDAAGANTGRLLPTG 185

Query: 182 NASDWI------DGLKVSIIDSAVPVVFIRQHDVGITGSEAPATLNANTALLDRLERVRL 235
              D I      DG++VS ID+A+PV+ +R  D+G TG E   +   +   + RLE +R+
Sbjct: 186 APVDRIIGIDGTDGIEVSCIDAAIPVMLVRAADLGKTGHEPMDSYRLDRVFMARLEALRV 245

Query: 236 EAGRRMGLGDVSGSVVPKLSLIGPGTETTTFTARYFTPKACHNAHAVTGAICTAGAAYID 295
           EAGRRMG  + +G V+PK  L+ P T   T   RYF P  CH A A+TGA+ TA A  I 
Sbjct: 246 EAGRRMGFPNAAGMVIPKPVLLAPPTRGGTLAVRYFMPHDCHRAMAITGAVATATACTIP 305

Query: 296 GSVVCEILSSRASACSASQRRISIEHPSGVLEVGLVPPENAAQSLVDVAVVERS 349
           G+V     +S  +   A    ++ EHP+G L V L P   A QS    +++  S
Sbjct: 306 GTV-----ASALAGRMALPGDVTFEHPAGRLTVRLDP--GAGQSAPTASILRTS 352


Lambda     K      H
   0.318    0.133    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 464
Number of extensions: 27
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 443
Length of database: 374
Length adjustment: 31
Effective length of query: 412
Effective length of database: 343
Effective search space:   141316
Effective search space used:   141316
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory