Align malonate-semialdehyde dehydrogenase (EC 1.2.1.15); malonate-semialdehyde dehydrogenase (acetylating) (EC 1.2.1.18); methylmalonate-semialdehyde dehydrogenase (CoA-acylating) (EC 1.2.1.27) (characterized)
to candidate AZOBR_RS22500 AZOBR_RS22500 methylmalonate-semialdehyde dehydrogenase
Query= BRENDA::A0A081YAY7 (498 letters) >FitnessBrowser__azobra:AZOBR_RS22500 Length = 499 Score = 742 bits (1915), Expect = 0.0 Identities = 357/497 (71%), Positives = 416/497 (83%), Gaps = 1/497 (0%) Query: 1 MTLIKHLIGGELIADTG-RTADVFNPSTGEAVRKVPLADRETMQQAIDAAKAAFPAWRNT 59 M L+ HLIGG A R+AD+ NP+TGE V +VPLA R T++ AI AA+AAFPAWR T Sbjct: 1 MPLVPHLIGGAADAPAETRSADIVNPATGETVGRVPLAGRATVESAIAAAEAAFPAWRAT 60 Query: 60 PPAKRAQVLFRFKQLLEANEERIVKLISEEHGKTIEDAAGELKRGIENVEYATAAPEILK 119 PPAKRA+V+FRF+QLLE N +R+ I+ EHGKT+EDA GEL RGIENVEYA ++LK Sbjct: 61 PPAKRARVMFRFRQLLEDNADRVCAAITREHGKTLEDARGELTRGIENVEYACGIADLLK 120 Query: 120 GEYSRNVGPNIDAWSDFQPIGVVAGITPFNFPAMVPLWMYPLAIACGNTFILKPSERDPS 179 GE+S+NVGP ID+WS+FQP+GVVAGITPFNFPAMVPLWM+P+A+ACGN FILKPSERDPS Sbjct: 121 GEHSKNVGPGIDSWSEFQPLGVVAGITPFNFPAMVPLWMFPVAVACGNCFILKPSERDPS 180 Query: 180 STLLIAELFHEAGLPKGVLNVVHGDKGAVDALIEAPEVKALSFVGSTPIAEYIYSEGTKR 239 ++LL+A+L EAGLP GVLNVVHGDK AVD L+ P V+A+SFVGSTP+AEY+Y+ GT Sbjct: 181 ASLLVAQLAQEAGLPPGVLNVVHGDKEAVDTLLTDPRVQAVSFVGSTPVAEYVYATGTAH 240 Query: 240 GKRVQALGGAKNHAVLMPDADLDNAVSALMGAAYGSCGERCMAISVAVCVGDQIADALVQ 299 GKRVQALGGAKNHA++MPDADLDNAVSA+MGAAYGSCGERCMAISV V VGD AD +V Sbjct: 241 GKRVQALGGAKNHAIVMPDADLDNAVSAIMGAAYGSCGERCMAISVVVAVGDATADRVVA 300 Query: 300 KLVPQIKGLKIGAGTSCGLDMGPLVTGAARDKVTGYIDTGVAQGAELVVDGRGYKVAGHE 359 L Q++ LK+GAGT G DMGPLVT A +KV G++D GVA+GAELVVDGRG V GHE Sbjct: 301 MLAEQVRSLKVGAGTGAGCDMGPLVTRAHFEKVKGFVDQGVAEGAELVVDGRGLVVPGHE 360 Query: 360 NGFFLGGTLFDRVTPEMTIYKEEIFGPVLCIVRVNSLEEAMQLINDHEYGNGTCIFTRDG 419 GFFLGG LFDRVTP+M IY+EEIFGPVLC+VRV +++E M LI+ HEYGNGTC+FTRDG Sbjct: 361 GGFFLGGCLFDRVTPDMRIYREEIFGPVLCVVRVATMQEGMDLIDAHEYGNGTCLFTRDG 420 Query: 420 EAARLFCDEIEVGMVGVNVPLPVPVAYHSFGGWKRSLFGDLHAYGPDGVRFYTKRKAITQ 479 EAAR F D I+VGMVG+NVPLPVPV+YHSFGGWKRSLFGDL AYGPDGVRFYT+RK ITQ Sbjct: 421 EAARYFTDAIKVGMVGINVPLPVPVSYHSFGGWKRSLFGDLAAYGPDGVRFYTRRKTITQ 480 Query: 480 RWPQRKSHEAAQFAFPS 496 RWP E AQF+FPS Sbjct: 481 RWPTGGVREGAQFSFPS 497 Lambda K H 0.319 0.137 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 851 Number of extensions: 28 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 498 Length of database: 499 Length adjustment: 34 Effective length of query: 464 Effective length of database: 465 Effective search space: 215760 Effective search space used: 215760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
Align candidate AZOBR_RS22500 AZOBR_RS22500 (methylmalonate-semialdehyde dehydrogenase)
to HMM TIGR01722 (mmsA: methylmalonate-semialdehyde dehydrogenase (acylating) (EC 1.2.1.27))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01722.hmm # target sequence database: /tmp/gapView.31683.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01722 [M=477] Accession: TIGR01722 Description: MMSDH: methylmalonate-semialdehyde dehydrogenase (acylating) Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.8e-210 684.5 0.4 4.3e-210 684.3 0.4 1.0 1 lcl|FitnessBrowser__azobra:AZOBR_RS22500 AZOBR_RS22500 methylmalonate-sem Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__azobra:AZOBR_RS22500 AZOBR_RS22500 methylmalonate-semialdehyde dehydrogenase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 684.3 0.4 4.3e-210 4.3e-210 1 477 [] 4 482 .. 4 482 .. 0.99 Alignments for each domain: == domain 1 score: 684.3 bits; conditional E-value: 4.3e-210 TIGR01722 1 vkhlidGkfvegksdkyipvsnpatnevlakvaeasaeevdaavasaretfaawaetsvaerarvllry 69 v hli+G+ + ++ ++ npat+e++++v+ a + v++a+a+a +f+aw+ t+ a+rarv++r+ lcl|FitnessBrowser__azobra:AZOBR_RS22500 4 VPHLIGGAADAPAETRSADIVNPATGETVGRVPLAGRATVESAIAAAEAAFPAWRATPPAKRARVMFRF 72 5699***9999999999**************************************************** PP TIGR01722 70 qallkehrdeiaklisaeqGktledakGdvarGlevvehacsvtslllGetvesvakdvdvysirqplG 138 ++ll+++ d + i+ e+Gktleda+G++ rG+e ve+ac+++ ll+Ge +++v +d +s qplG lcl|FitnessBrowser__azobra:AZOBR_RS22500 73 RQLLEDNADRVCAAITREHGKTLEDARGELTRGIENVEYACGIADLLKGEHSKNVGPGIDSWSEFQPLG 141 ********************************************************************* PP TIGR01722 139 vvaGitpfnfpamiplwmfplaiacGntfvlkpsekvpsaavklaellseaGapdGvlnvvhGdkeavd 207 vvaGitpfnfpam+plwmfp+a+acGn+f+lkpse++psa++ +a+l +eaG+p GvlnvvhGdkeavd lcl|FitnessBrowser__azobra:AZOBR_RS22500 142 VVAGITPFNFPAMVPLWMFPVAVACGNCFILKPSERDPSASLLVAQLAQEAGLPPGVLNVVHGDKEAVD 210 ********************************************************************* PP TIGR01722 208 rllehpdvkavsfvGsvavgeyiyetgsahgkrvqalaGaknhmvvlpdadkeaaldalvgaavGaaGq 276 +ll p v+avsfvGs++v+ey+y tg+ahgkrvqal+Gaknh++v+pdad+++a++a++gaa+G+ G+ lcl|FitnessBrowser__azobra:AZOBR_RS22500 211 TLLTDPRVQAVSFVGSTPVAEYVYATGTAHGKRVQALGGAKNHAIVMPDADLDNAVSAIMGAAYGSCGE 279 ********************************************************************* PP TIGR01722 277 rcmaisaavlvGaa..kelveeireraekvrvgagddpgaelGplitkqakervasliasgakeGaevl 343 rcmais++v+vG+a +++v + e+++ ++vgag+ +g+++Gpl+t+++ e+v+ ++++g++eGae++ lcl|FitnessBrowser__azobra:AZOBR_RS22500 280 RCMAISVVVAVGDAtaDRVVAMLAEQVRSLKVGAGTGAGCDMGPLVTRAHFEKVKGFVDQGVAEGAELV 348 ************987799*************************************************** PP TIGR01722 344 ldGrgykveGyeeGnfvGitllervkpdmkiykeeifGpvlvvleadtleeaiklinespyGnGtaift 412 +dGrg+ v G+e G f+G +l++rv pdm+iy+eeifGpvl+v+++ t++e++ li++ yGnGt++ft lcl|FitnessBrowser__azobra:AZOBR_RS22500 349 VDGRGLVVPGHEGGFFLGGCLFDRVTPDMRIYREEIFGPVLCVVRVATMQEGMDLIDAHEYGNGTCLFT 417 ********************************************************************* PP TIGR01722 413 sdGaaarkfqheievGqvGvnvpipvplpffsftGwkdslfGdlhiyGkqGvrfytrlktvtarw 477 +dG aar f i+vG+vG+nvp+pvp++++sf+Gwk slfGdl +yG +Gvrfytr kt+t rw lcl|FitnessBrowser__azobra:AZOBR_RS22500 418 RDGEAARYFTDAIKVGMVGINVPLPVPVSYHSFGGWKRSLFGDLAAYGPDGVRFYTRRKTITQRW 482 ***************************************************************** PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (477 nodes) Target sequences: 1 (499 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 12.68 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory