Alignments for a candidate for mmsA in Azospirillum brasilense Sp245

GapMind for catabolism of small carbon sources

Alignments for a candidate for mmsA in Azospirillum brasilense Sp245

Align Malonate-semialdehyde dehydrogenase 1; MSA dehydrogenase 1; EC 1.2.1.-; Methylmalonate-semialdehyde dehydrogenase 1; MMSA dehydrogenase 1; MSDH 1; EC 1.2.1.27 (uncharacterized)
to candidate AZOBR_RS29185 AZOBR_RS29185 aldehyde dehydrogenase

Query= curated2:Q81QR5
         (486 letters)



>FitnessBrowser__azobra:AZOBR_RS29185
          Length = 478

 Score =  253 bits (647), Expect = 8e-72
 Identities = 166/472 (35%), Positives = 237/472 (50%), Gaps = 10/472 (2%)

Query: 10  KNHINGEWVESTGTEVEAVPNPAT-GKIIAYVPLSPKEDVEKAVEAAKAAYETWSKVPVP 68
           +N I G  V+  G  +EA  NPA  G++I +   +  E  E+A+ AA AA   W+     
Sbjct: 5   RNWIAGRLVD--GVRMEANRNPARPGEVIGHYAWASVEQAEEALGAAHAALPGWAASNPQ 62

Query: 69  NRSRQLYKYLQLLQENKEELAKIITLENGKTLTDATGEVQRGIEAVELATSAPNLMMGQA 128
            RS  L +    L    EEL  ++T E GKTL +  GEV+R  +    A   P    G+A
Sbjct: 63  TRSDVLRRVGDELNARAEELGALLTREEGKTLREGIGEVRRSAQIFHYAAGEPLRQGGEA 122

Query: 129 LPNIASGIDGSIWRYPIGVVAGITPFNFPMMIPLWMFPLAIACGNTFVLKTSERTPLLAE 188
           LP +  G    + R P+GVV  ITP+NFPM +P W    A+A GNT VLK SE TP  A 
Sbjct: 123 LPGLRDGTTAMVSREPVGVVVLITPWNFPMAVPAWKTAYALAFGNTVVLKPSEVTPACAW 182

Query: 189 RLVELFYEAGFPKGVLNLVQG-GKDVVNSILENKDIQAVSFVGSEPVARYVYETGTKHGK 247
            L ++ + AG P G  NLV G G+ +  ++++  D  AVSF GS  V R + E       
Sbjct: 183 ELADILHRAGLPAGAFNLVVGDGRTLGPALVDGAD--AVSFTGSPGVGRAILERSVARMT 240

Query: 248 RVQALAGAKNHAIVMPDCNLEKTVQGVIGSAFASSGERCMACSVVAVVDEIADEFIDVLV 307
           RVQ   G KN  +V  D +LE  V   +  AF S+G+RC A S + V   + D F++ LV
Sbjct: 241 RVQLELGGKNPLVVHDDADLELAVDIALQGAFHSTGQRCTATSRIIVDRRVHDAFVERLV 300

Query: 308 AETKKLKVGDGFHEDNYVGPLIRESHKERVLGYINSGVADGATLLVDGRKIKEEVGEGYF 367
                L+VGD       +GP++ E+   + L  I    ++GA L   G ++    G+GYF
Sbjct: 301 TRIAALRVGDPMDAATDMGPVVSEAQLAKDLHCIADARSEGAELAFGGGRMD---GDGYF 357

Query: 368 VGATIFDGVNQEMKIWQDEIFAPVLSIVRVKDLEEGIKLTNQSKFANGAVIYTSNGKHAQ 427
           +  T+F G +  M+I +DE+F PV  ++    L+  I + N S  A  + I T     A+
Sbjct: 358 LEPTLFVGTDNAMRINRDEVFGPVACVIPADGLDHAIAIANDSDHALSSGIVTRGLASAE 417

Query: 428 TFRDNIDAGMIGVNVNVPAPMAFFAFAGNKASFFGDLGTNGTDGVQFYTRKK 479
           TFR    AG++ VN           F G   S +G     G+  V+F+T  K
Sbjct: 418 TFRRRSRAGLVMVNAPTAGIDYHVPFGGRGPSGYGG-REQGSAAVEFFTEGK 468


Lambda     K      H
   0.317    0.135    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 546
Number of extensions: 28
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 486
Length of database: 478
Length adjustment: 34
Effective length of query: 452
Effective length of database: 444
Effective search space:   200688
Effective search space used:   200688
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources