Protein GFF850 in Pseudomonas stutzeri RCH2
Annotation: FitnessBrowser__psRCH2:GFF850
Length: 521 amino acids
Source: psRCH2 in FitnessBrowser
Candidate for 11 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| D-maltose catabolism | malF | hi | Maltose-transporting ATPase (EC 3.6.3.19) (characterized) | 100% | 100% | 1027.7 | MalF1; aka Maltose ABC transporter, permease protein, component of The maltose, maltotriose, mannotetraose (MalE1)/maltose, maltotriose, trehalose (MalE2) porter (Nanavati et al., 2005). For MalG1 (823aas) and MalG2 (833aas), the C-terminal transmembrane domain with 6 putative TMSs is preceded by a single N-terminal TMS and a large (600 residue) hydrophilic region showing sequence similarity to MLP1 and 2 (9.A.14; e-12 & e-7) as well as other proteins | 41% | 266.2 |
| D-maltose catabolism | malF1 | lo | MalF1; aka Maltose ABC transporter, permease protein, component of The maltose, maltotriose, mannotetraose (MalE1)/maltose, maltotriose, trehalose (MalE2) porter (Nanavati et al., 2005). For MalG1 (823aas) and MalG2 (833aas), the C-terminal transmembrane domain with 6 putative TMSs is preceded by a single N-terminal TMS and a large (600 residue) hydrophilic region showing sequence similarity to MLP1 and 2 (9.A.14; e-12 & e-7) as well as other proteins (characterized) | 41% | 66% | 266.2 | Maltose-transporting ATPase (EC 3.6.3.19) | 100% | 1027.7 |
| trehalose catabolism | malF1 | lo | MalF1; aka Maltose ABC transporter, permease protein, component of The maltose, maltotriose, mannotetraose (MalE1)/maltose, maltotriose, trehalose (MalE2) porter (Nanavati et al., 2005). For MalG1 (823aas) and MalG2 (833aas), the C-terminal transmembrane domain with 6 putative TMSs is preceded by a single N-terminal TMS and a large (600 residue) hydrophilic region showing sequence similarity to MLP1 and 2 (9.A.14; e-12 & e-7) as well as other proteins (characterized) | 41% | 66% | 266.2 | Maltose-transporting ATPase (EC 3.6.3.19) | 100% | 1027.7 |
| D-maltose catabolism | malF_Aa | lo | Binding-protein-dependent transport systems inner membrane component (characterized, see rationale) | 39% | 78% | 183.7 | Maltose-transporting ATPase (EC 3.6.3.19) | 100% | 1027.7 |
| D-maltose catabolism | malF_Sm | lo | MalF, component of Maltose/Maltotriose/maltodextrin (up to 7 glucose units) transporters MalXFGK (MsmK (3.A.1.1.28) can probably substitute for MalK; Webb et al., 2008) (characterized) | 35% | 55% | 149.1 | Maltose-transporting ATPase (EC 3.6.3.19) | 100% | 1027.7 |
| trehalose catabolism | malF | lo | MalF, component of Maltose/Maltotriose/maltodextrin (up to 7 glucose units) transporters MalXFGK (MsmK (3.A.1.1.28) can probably substitute for MalK; Webb et al., 2008) (characterized) | 35% | 55% | 149.1 | Maltose-transporting ATPase (EC 3.6.3.19) | 100% | 1027.7 |
| D-maltose catabolism | thuF | lo | Putative maltose permease, component of MalEFG (K unknown), involved in maltose and maltodextrin uptake (characterized) | 33% | 69% | 134.4 | Maltose-transporting ATPase (EC 3.6.3.19) | 100% | 1027.7 |
| trehalose catabolism | thuF | lo | Trehalose/maltose transport system permease protein MalF (characterized) | 34% | 81% | 124 | Maltose-transporting ATPase (EC 3.6.3.19) | 100% | 1027.7 |
| D-glucosamine (chitosamine) catabolism | SM_b21220 | lo | ABC transporter for D-Glucosamine, permease component 2 (characterized) | 30% | 83% | 123.2 | Maltose-transporting ATPase (EC 3.6.3.19) | 100% | 1027.7 |
| sucrose catabolism | thuF | lo | ABC transporter permease (characterized, see rationale) | 30% | 78% | 112.8 | Maltose-transporting ATPase (EC 3.6.3.19) | 100% | 1027.7 |
| trehalose catabolism | treT | lo | TreT, component of Trehalose porter (characterized) | 31% | 77% | 92.8 | Maltose-transporting ATPase (EC 3.6.3.19) | 100% | 1027.7 |
Sequence Analysis Tools
View GFF850 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MNARAELPSPAMTRPTSWGLPRFLTTGVRWLLWLAFNALSLYLVVALYVQKQMAFALLGL
VVTGIASYLFINRRMYAQRYIFPSVAGMLVFVIFPLLYTVGIGFTNYSGTNLLSQAQVER
YHLSQTYLAGERFRFTLHQSPDGERLRVDKGELGVFVSPPLTGEPDPEAPLSLLPAESVE
GLGEALALREVIQRRKVLEQWVMQAPDGSLLRLYGLREVAAVEPQYRQDGPGVLVETRTG
ARLTADMERGFYVDETGKAVPPGFTVFTGFANFSRVLTEPSIREPFMQIFAWTFAFAGLT
VVFTLAVGLVLASLLQWELVRGKAFYRLMLILPYAVPGFISILVFRGLFNQNFGEINLLL
EGLFGIRPDWFSDPSLARTMILIVNTWLGYPYMLLLCMGLLQAIPRDQYEASAIDGASPL
DNLLRITLPQLIKPLMPLLIACFAFNFNNFVLITLLTRGGPDIIGATTPAGTTDLLVSYT
YRIAFQDSGQDFALAAAIATMIFILVGAMALLNLKLSKVKV
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory