Alignments for a candidate for hcl in Pseudomonas stutzeri RCH2

GapMind for catabolism of small carbon sources

Alignments for a candidate for hcl in Pseudomonas stutzeri RCH2

Align 4-hydroxybenzoate--CoA/benzoate--CoA ligase; 4-hydroxybenzoyl-CoA synthetase; EC 6.2.1.25; EC 6.2.1.27 (characterized)
to candidate GFF2177 Psest_2220 Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Query= SwissProt::Q53005
         (539 letters)



>FitnessBrowser__psRCH2:GFF2177
          Length = 585

 Score =  195 bits (495), Expect = 5e-54
 Identities = 167/555 (30%), Positives = 251/555 (45%), Gaps = 38/555 (6%)

Query: 1   MPLRDYNAAVDFVDRNVAEGRGGKIA--FID---PQRSLSYGELRDAVARVGPMLARLGV 55
           +P    N A + VDR+ A  R G  A  F++    +R LSY +L+    R   +LA+LGV
Sbjct: 41  LPGGGLNLAHEAVDRHAAGPRAGYCALRFLERNGSRRELSYLQLKRQTNRFANVLAQLGV 100

Query: 56  EQENRIALVLKDTVDFPILFWGAIRAGIV---------PVLLNTRLTADQYRYLLEDSRS 106
            +  R+ ++    V+  +   G ++ G V         P  L TRL       LL    S
Sbjct: 101 ARGERLFVLCNRGVELYLGVLGGLKNGCVVSPLFAAFGPEPLETRLRLGDCSVLLT---S 157

Query: 107 RVVFASSEFLPVIEEAAADLPHLRTIIAVGDAPAPT-----LQLANLLATEQEGGAPAAT 161
            +++        +      LP L+ ++   ++   T     L L +LLA   E    A T
Sbjct: 158 EMLYRKK-----VARLRDRLPLLKHVLLYDESGGHTSIDGTLDLHDLLAAAGEEFKIAPT 212

Query: 162 CADDIAYWQYSSGTTGMPKGVMHVHSSPRVMAENAGRRIGYREDDVVFSAAKLFFAYGLG 221
            AD  +   ++SGTTG PKG +HVH +           +    DD+ + +A   +  G  
Sbjct: 213 TADSPSLLHFTSGTTGTPKGALHVHGAALTHYVTGRYALDLHPDDIYWCSADPGWVTGTS 272

Query: 222 NAMFCPMGIGATSVLYPERPTADSVFDTLRLHQPTLLFAVPT-LYAAMLADPRSRTETLP 280
             +  P+ +G TSV+      A+  +  L   + ++ +  PT +   M A P        
Sbjct: 273 YGILAPLLLGVTSVVDCGEFDAERWYGILEKERISVWYTAPTAIRMLMKAGPELARRHFH 332

Query: 281 DRLRLCVSAGEPLPAQVGLNWRNRFGHDIVNGVGSTEMGHLFLTNLPHA-VEYGTSGVPV 339
            +LR   S GEPL  +     +   G  I +    TE G + + N+P   ++ G+ G P+
Sbjct: 333 PQLRFVASVGEPLNPEAVWWGKEVLGLPIHDNWWQTETGGIMIANIPAMPIKPGSMGKPL 392

Query: 340 DGYRLRLVGDRGQD-----VADDEIGELLVSGG--SSAAGYWNQRDKTRTTFVGEWTRTG 392
            G    +V   G+D     + DDEIGEL +          Y  Q ++ R  F G+W  +G
Sbjct: 393 PGVEAAIVR-HGEDGNLTFLGDDEIGELALKQPWPGMFRTYLGQEERYRRCFAGDWYLSG 451

Query: 393 DKYHRRADGVYTYCGRTDDIFKVSGIWVSPFEIEQALMSHAKVLEAAVIPAEDTDGLIKP 452
           D   R ADG Y + GR+DD+ K +G  + PFE+E ALM H+ V EAAVI   D       
Sbjct: 452 DLARRDADGYYWFIGRSDDVIKSAGHLIGPFEVESALMEHSAVAEAAVIGKPDPLLGETV 511

Query: 453 KAFIVLASRGDIDPGALFDELKEHVKSAIGPWKYPRWIQIMDDLPKTSSGKLQRYLLREM 512
           KAF+ L  RG     AL DEL  H +  +G    P+ ++ +  LP+T SGKL R LL+  
Sbjct: 512 KAFVSL-KRGREASEALHDELIGHARKRLGAAVAPKELEFLASLPRTRSGKLMRRLLKAR 570

Query: 513 TLGGIEATESAPSEP 527
            LG  E   S    P
Sbjct: 571 ELGLPEGDISTLENP 585


Lambda     K      H
   0.320    0.138    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 831
Number of extensions: 48
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 539
Length of database: 585
Length adjustment: 36
Effective length of query: 503
Effective length of database: 549
Effective search space:   276147
Effective search space used:   276147
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources