Align arginine deiminase (EC 3.5.3.6) (characterized)
to candidate GFF271 Psest_0272 arginine deiminase
Query= BRENDA::P13981 (418 letters) >FitnessBrowser__psRCH2:GFF271 Length = 417 Score = 701 bits (1809), Expect = 0.0 Identities = 331/415 (79%), Positives = 376/415 (90%) Query: 4 EKTKLGVHSEAGKLRKVMVCSPGLAHQRLTPSNCDELLFDDVIWVNQAKRDHFDFVTKMR 63 + KLGVHSEAGKLRKVMVCSPGLAHQRLTPSNCDELLFDDV+WV+QAKRDHFDFV+KMR Sbjct: 3 QSPKLGVHSEAGKLRKVMVCSPGLAHQRLTPSNCDELLFDDVLWVSQAKRDHFDFVSKMR 62 Query: 64 ERGIDVLEMHNLLTETIQNPEALKWILDRKITADSVGLGLTSELRSWLESLEPRKLAEYL 123 ERG++VLEMHNLLTET+ +PEALKW+LDRKITA+ VGLGL E+RSWLE LE R+LAE+L Sbjct: 63 ERGVEVLEMHNLLTETLSDPEALKWLLDRKITANQVGLGLQGEVRSWLEGLERRQLAEFL 122 Query: 124 IGGVAADDLPASEGANILKMYREYLGHSSFLLPPLPNTQFTRDTTCWIYGGVTLNPMYWP 183 IGGV+ DLP SEG I++M+R+YLGHSSF+LPPLPNT FTRDTTCWIYGGV+LNPM+W Sbjct: 123 IGGVSGSDLPGSEGGEIVRMFRDYLGHSSFILPPLPNTLFTRDTTCWIYGGVSLNPMHWT 182 Query: 184 ARRQETLLTTAIYKFHPEFANAEFEIWYGDPDKDHGSSTLEGGDVMPIGNGVVLIGMGER 243 ARRQETLLT AIY+FHP+F + +F++WYGDP+ DHG +TLEGGDVMPIG GVVLIGMGER Sbjct: 183 ARRQETLLTAAIYRFHPQFRDQDFKVWYGDPELDHGLATLEGGDVMPIGKGVVLIGMGER 242 Query: 244 SSRQAIGQVAQSLFAKGAAERVIVAGLPKSRAAMHLDTVFSFCDRDLVTVFPEVVKEIVP 303 SSRQAIGQ+AQSLF GAAERVIVAGLP+SR+AMHLDTVFSFCDRDLVT+FPEVV I Sbjct: 243 SSRQAIGQLAQSLFRHGAAERVIVAGLPRSRSAMHLDTVFSFCDRDLVTIFPEVVHGITA 302 Query: 304 FSLRPDPSSPYGMNIRREEKTFLEVVAESLGLKKLRVVETGGNSFAAEREQWDDGNNVVC 363 FSLRPD P G+++RRE+ FL+VVAE+LGL LRVVETGG+S+ EREQWDDGNNVV Sbjct: 303 FSLRPDERKPDGIDLRREQAPFLDVVAEALGLTALRVVETGGDSYEVEREQWDDGNNVVA 362 Query: 364 LEPGVVVGYDRNTYTNTLLRKAGVEVITISASELGRGRGGGHCMTCPIVRDPIDY 418 LEPGVV+GYDRNT+TNT+LRKAGVEVITISASELGRGRGGGHCMTCPI+RD IDY Sbjct: 363 LEPGVVIGYDRNTFTNTMLRKAGVEVITISASELGRGRGGGHCMTCPIIRDAIDY 417 Lambda K H 0.319 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 680 Number of extensions: 20 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 418 Length of database: 417 Length adjustment: 32 Effective length of query: 386 Effective length of database: 385 Effective search space: 148610 Effective search space used: 148610 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate GFF271 Psest_0272 (arginine deiminase)
to HMM TIGR01078 (arcA: arginine deiminase (EC 3.5.3.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01078.hmm # target sequence database: /tmp/gapView.4477.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01078 [M=408] Accession: TIGR01078 Description: arcA: arginine deiminase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.7e-168 545.9 0.0 3e-168 545.7 0.0 1.0 1 lcl|FitnessBrowser__psRCH2:GFF271 Psest_0272 arginine deiminase Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__psRCH2:GFF271 Psest_0272 arginine deiminase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 545.7 0.0 3e-168 3e-168 3 408 .] 9 415 .. 7 415 .. 0.99 Alignments for each domain: == domain 1 score: 545.7 bits; conditional E-value: 3e-168 TIGR01078 3 vysEiGkLkkvllhrPgkelenltPsnldelLFddilavedakkeHdeFveklrdngvevllledLlaEtlaasea 78 v+sE+GkL+kv++++Pg ++++ltPsn+delLFdd+l+v++ak++H +Fv+k+r++gvevl++++Ll+Etl+ +ea lcl|FitnessBrowser__psRCH2:GFF271 9 VHSEAGKLRKVMVCSPGLAHQRLTPSNCDELLFDDVLWVSQAKRDHFDFVSKMRERGVEVLEMHNLLTETLSDPEA 84 9*************************************************************************** PP TIGR01078 79 keklldealeeaeilgeelkkelreylksletrelvekllaGvakeelpeeedselslvdlevegdsefvidPlPn 154 ++lld+++++++ +g +l+ e+r++l+ le r+l+e l++Gv ++lp +e e+ ++ + + g+s+f+++PlPn lcl|FitnessBrowser__psRCH2:GFF271 85 LKWLLDRKITANQ-VGLGLQGEVRSWLEGLERRQLAEFLIGGVSGSDLPGSEGGEIVRMFRDYLGHSSFILPPLPN 159 *************.9************************************************************* PP TIGR01078 155 llFtRDpsasigngvtlnkmalkaRqrEtlftelifkhHpkfanaevevyyd....rsekaslEGGDvlvlskdvl 226 +lFtRD++++i++gv+ln+m + aR++Etl+t +i+++Hp+f +++++v+y+ ++ a+lEGGDv+ ++k+v+ lcl|FitnessBrowser__psRCH2:GFF271 160 TLFTRDTTCWIYGGVSLNPMHWTARRQETLLTAAIYRFHPQFRDQDFKVWYGdpelDHGLATLEGGDVMPIGKGVV 235 ***************************************************999999999**************** PP TIGR01078 227 viGiseRtsaqsveklakslfkneaefkkvlaiklpkkralmHLDtvltmvDkdkftvfpevekelkafsiysltk 302 +iG++eR+s+q++++la+slf + +++++v+++ lp++r++mHLDtv++++D+d +t+fpev++ ++afs++ +++ lcl|FitnessBrowser__psRCH2:GFF271 236 LIGMGERSSRQAIGQLAQSLFRH-GAAERVIVAGLPRSRSAMHLDTVFSFCDRDLVTIFPEVVHGITAFSLRPDER 310 ***********************.**************************************************** PP TIGR01078 303 gneekikveekakleevlaealgvkklkliktgGgdsveaeREqwnDgnnvlaiaPGvvvaYeRnvvtnallekaG 378 ++++ ++e+a++ +v+aealg+ l++++tg gds+e eREqw+Dgnnv+a++PGvv++Y+Rn+ tn++l+kaG lcl|FitnessBrowser__psRCH2:GFF271 311 KPDGIDLRREQAPFLDVVAEALGLTALRVVETG-GDSYEVEREQWDDGNNVVALEPGVVIGYDRNTFTNTMLRKAG 385 *********************************.9***************************************** PP TIGR01078 379 ikvltiagseLsrGrGgprCmsmplvRddi 408 ++v+ti++seL+rGrGg++Cm++p+ Rd i lcl|FitnessBrowser__psRCH2:GFF271 386 VEVITISASELGRGRGGGHCMTCPIIRDAI 415 ***************************976 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (408 nodes) Target sequences: 1 (417 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 10.25 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory