Alignments for a candidate for agcS in Pseudomonas stutzeri RCH2

GapMind for catabolism of small carbon sources

Alignments for a candidate for agcS in Pseudomonas stutzeri RCH2

Align Probable Glycine/alanine/asparagine/glutamine uptake porter, AgcS (characterized)
to candidate GFF2091 Psest_2134 amino acid carrier protein

Query= TCDB::W0WFC6
         (449 letters)



>FitnessBrowser__psRCH2:GFF2091
          Length = 447

 Score =  730 bits (1885), Expect = 0.0
 Identities = 353/447 (78%), Positives = 403/447 (90%)

Query: 1   MESLQKWVVDLNGVVWGPLMLVLILGTGLYLMLGLKFMPLVRLGVGFRLLWQGRSKDDES 60
           ME L   V  +NG+VWGP MLVLILGTGL+LM+ LKFMPL R+  GF L+W+GR+K DE+
Sbjct: 1   MEFLNNLVNSVNGLVWGPPMLVLILGTGLFLMIFLKFMPLTRIPTGFALMWRGRTKGDEA 60

Query: 61  SGEISPFQALMTCLAATVGTGNIAGVATAIFLGGPGALFWMWCTALVGMATKFSEVVLAV 120
           +GEISPFQALMT LAATVGTGNIAGVATAIFLGGPGALFWMWCTALVGMATK+ EVVLAV
Sbjct: 61  TGEISPFQALMTSLAATVGTGNIAGVATAIFLGGPGALFWMWCTALVGMATKYCEVVLAV 120

Query: 121 HYREKDERNEHVGGPMYAIKNGLGKRWAWLGAAFALFGGLAGFGIGNMVQVNSMADALEV 180
           HYREKD+R EHVGGPMYAIKNGLGK+W WLG AFA+FGGLAGFGIGNMVQVNSMA ALE 
Sbjct: 121 HYREKDDRGEHVGGPMYAIKNGLGKKWIWLGTAFAIFGGLAGFGIGNMVQVNSMAHALET 180

Query: 181 SFGVPDWVTGVATMLVTGLVILGGIRRIGKVAEALVPFMCVGYIVASVIVLVVHAEAIPG 240
           +F VP W TG+ TM++ GLVILGGIRRIG VA +LVPFMC+ Y++A+ +VLVV+  AIP 
Sbjct: 181 TFAVPLWATGLVTMVIVGLVILGGIRRIGVVAASLVPFMCLAYLIAAAVVLVVNVSAIPA 240

Query: 241 AFQLIFTHAFTPIAATGGFAGAAVMAAIRFGVARGIFSNEAGLGTAGIAQAAGTTHSAVR 300
           AF LIFTHAFTPIAATGGFAGAAVMAAIRFGVARGIFSNEAGLGTAGIAQAAGTT S+VR
Sbjct: 241 AFDLIFTHAFTPIAATGGFAGAAVMAAIRFGVARGIFSNEAGLGTAGIAQAAGTTTSSVR 300

Query: 301 SGLIGMLGTFIDTLIICSLTGLAIITSGVWTSGASGAALSSAAFEAAMPGVGHYILSLAL 360
           SG+IGMLGTFIDT+I+CS+TGLAII +GVWTSG SGAALS+AAFE+AMPG+G  IL++AL
Sbjct: 301 SGMIGMLGTFIDTIIVCSMTGLAIICTGVWTSGESGAALSAAAFESAMPGIGGIILTIAL 360

Query: 361 VVFAYTTILGWSYYGERCWEYLAGTRAILPFRIVWTLAIPFGAMTQLDFAWLVADTLNAL 420
           VVFA+TTILGWSY+GE+CWE+L GTRAI PFR++W LA+PFGA+ QLDFAWL+ADTLN L
Sbjct: 361 VVFAFTTILGWSYFGEKCWEFLVGTRAIWPFRVIWVLAVPFGAIAQLDFAWLLADTLNGL 420

Query: 421 MAIPNLIALLLLSPVVFRLTREYFAKA 447
           MAIPNLI+LLLLSPVV +LT+EYFA++
Sbjct: 421 MAIPNLISLLLLSPVVVKLTKEYFARS 447


Lambda     K      H
   0.326    0.140    0.437 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 799
Number of extensions: 38
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 449
Length of database: 447
Length adjustment: 33
Effective length of query: 416
Effective length of database: 414
Effective search space:   172224
Effective search space used:   172224
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources