GapMind for catabolism of small carbon sources

 

Alignments for a candidate for PS417_17605 in Pseudomonas stutzeri RCH2

Align ATP-binding cassette domain-containing protein; SubName: Full=Amino acid transporter; SubName: Full=Histidine ABC transporter ATP-binding protein; SubName: Full=Histidine transport system ATP-binding protein (characterized, see rationale)
to candidate GFF16 Psest_0016 ectoine/hydroxyectoine ABC transporter, ATP-binding protein

Query= uniprot:A0A1N7U8S3
         (276 letters)



>FitnessBrowser__psRCH2:GFF16
          Length = 278

 Score =  227 bits (579), Expect = 2e-64
 Identities = 132/273 (48%), Positives = 174/273 (63%), Gaps = 9/273 (3%)

Query: 3   QSAQALAAYPVDEPVAQPVTAAIKLQVEGIHKRYGEHEVLKGVSLNARQGDVISLIGASG 62
           QS   L   P     AQP+     ++  G+ KRYGE  VL G+ L   +G+ +++IG SG
Sbjct: 6   QSTDTLNPTPQQPEQAQPL-----VRFAGVTKRYGELTVLDGLDLQIEEGEKVAIIGPSG 60

Query: 63  SGKSTMLRCINFLEQPDAGVITLDGISIEMRQGRAGTRAP-HQDQLQNLRTRLAMVFQHF 121
           SGKST+LR +  LE  D GVI +DG  +      +G   P +   L+ +R ++ MVFQ F
Sbjct: 61  SGKSTLLRVLMTLEGIDEGVIEVDGEPLTHMPDASGQLVPANARHLRRVRGKVGMVFQSF 120

Query: 122 NLWSHMTVLENITMAPRRVLDVSAAEAEKRARMYLDKVGLPSRVADQYPAFLSGGQQQRV 181
           NL+ HM  L+N+  AP +VL +S AEA  RA   L  VGL  ++ + +PA LSGGQQQRV
Sbjct: 121 NLFPHMNALQNVMEAPVQVLGLSKAEARGRAEELLAMVGLEDKL-EHFPAQLSGGQQQRV 179

Query: 182 AIARALAMEPEIILFDEPTSALDPELVGEVLKVIQTLAE-EGRTMLMVTHEMGFARQVSS 240
           AIARALAM P+++LFDE TSALDPEL GEVL VI+ L E    TMLMVTH+MGFAR+ + 
Sbjct: 180 AIARALAMRPKVMLFDEVTSALDPELCGEVLNVIRRLGEAHNLTMLMVTHQMGFAREFAD 239

Query: 241 QVLFLHQGRVEEHGDA-RILDQPNSERLQQFLS 272
           +V F HQGR+ E G    + + P  ER ++FLS
Sbjct: 240 RVCFFHQGRIHEQGSPDELFNNPQEERTREFLS 272


Lambda     K      H
   0.319    0.133    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 239
Number of extensions: 13
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 276
Length of database: 278
Length adjustment: 25
Effective length of query: 251
Effective length of database: 253
Effective search space:    63503
Effective search space used:    63503
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory