Align alcohol dehydrogenase (quinone) (EC 1.1.5.5) (characterized)
to candidate GFF2017 Psest_2060 PQQ-dependent dehydrogenase, methanol/ethanol family
Query= BRENDA::Q44002 (739 letters) >FitnessBrowser__psRCH2:GFF2017 Length = 592 Score = 339 bits (869), Expect = 3e-97 Identities = 219/611 (35%), Positives = 299/611 (48%), Gaps = 67/611 (10%) Query: 8 KRRSLSRTLTAGTICAALISGYATMASADDGQGATGEAIIHADDHPGNWMTYGRTYSEQR 67 K L + +CAA+ S+ T + I++ +T G QR Sbjct: 2 KHTGLRKPFALTALCAAVAM------SSLHAWAVTDQEILNDAKSTDQIVTNGMGLQGQR 55 Query: 68 YSPLDQINRSNVGNLKLAWYLDL--DTNRGQEGTPLVIDGVMYATTNWSMMKAVDAATGK 125 YS LD +N +N+ L+ W L + RGQE PL+ DGVMY T ++S + AVDA TGK Sbjct: 56 YSTLDALNANNINQLRPVWGFSLGGEKQRGQEAQPLIKDGVMYITGSYSRVYAVDARTGK 115 Query: 126 LLWSYDPRVPGNIADKGCCDTVNRGAAYWNGKVYFGTFDGRLIALDAKTGKLVWSVNTIP 185 LW YD R+P I CCD +NRG A ++ V FGT D +L+AL+ TGK+VW Sbjct: 116 ELWQYDARLPDGIMP--CCDVINRGVALYDDLVIFGTLDAKLVALNKDTGKVVWKKTVAD 173 Query: 186 PEAELGKQRSYTVDGAPRIAKGRVIIGNGGSEFGARGFVTAFDAETGKVDWRFFTAPNPK 245 +A Y++ AP I KG++I G G EFG G + A++ + G++ W T P + Sbjct: 174 YKA------GYSLTAAPLIVKGKLITGVSGGEFGVVGKIEAYNPKNGELLW---TRPTVE 224 Query: 246 NEPDHTASDS------VLMNKAYQTWSPTGAWTRQGGGGTVWDSIVYDPVADLVYLGVGN 299 + D + +A +TW P W + GG W YDP D + G GN Sbjct: 225 GHMGYVWKDGKKVESGISGGEAGKTW-PGDLW--KTGGAAPWLGGYYDPDTDSLLFGTGN 281 Query: 300 GSPWNYKYRSEGKGDNLFLGSIVALKPETGEYVWHFQETPMDQWDFTSVQQIMTLDLPIN 359 +PWN R GDNL+ S +AL P G WHFQ TP D WD+ V ++++ D Sbjct: 282 PAPWNSHLRP---GDNLYSSSRLALDPNDGSIKWHFQTTPHDGWDYDGVNELVSFDYQEG 338 Query: 360 GETRHVIVHAPKNGFFYIIDAKTGEFISGKNYV-YVNWASGLDPKTGRPIYNP------- 411 G+T A +NGFFY++D G+FI G +V + WA GLD K GRPIY+ Sbjct: 339 GKTIKAAGTADRNGFFYVLDRTNGKFIRGFPFVDKITWAKGLD-KNGRPIYDDANRPGSP 397 Query: 412 -DALYTLTGKEWYGIPGDLGGHNFAAMAFSPKTGLVYIPAQQVPFLYTNQVGGFTPHPDS 470 DA GK P LGG N+ MA+S TGL Y+P+ + D Sbjct: 398 NDA--GAQGKTVTVAPSFLGGKNWMPMAYSQDTGLFYVPSNEWSM-------------DI 442 Query: 471 WNLGLDMNK------VGIPDSPEAKQAFVKDLKGWIVAWDPQKQAEAWRVDHKGPWNGGI 524 WN G K G P D G + A DP+ E WR + P GG+ Sbjct: 443 WNEGTAYKKGAAYLGAGFTIHP-----LNDDYIGVLRAIDPKTGKEVWRYKNYAPLWGGV 497 Query: 525 LATGGDLLFQGLANGEFHAYDATNGSDLFHFAADSGIIAPPVTYLANGKQYVAVEVGWGG 584 LAT G+L+F G G A+DA G L+ F SG+I PVT+ +G+QYV+V GWGG Sbjct: 498 LATKGNLVFTGNPEGYLMAFDAKTGKKLYEFNTGSGVIGSPVTWEMDGEQYVSVLSGWGG 557 Query: 585 IYPFFLGGLAR 595 P + G +A+ Sbjct: 558 AVPLWGGEVAK 568 Lambda K H 0.318 0.137 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1908 Number of extensions: 182 Number of successful extensions: 21 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 739 Length of database: 592 Length adjustment: 38 Effective length of query: 701 Effective length of database: 554 Effective search space: 388354 Effective search space used: 388354 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory