Align quinoprotein decanol dehydrogenase; EC 1.1.-.- (characterized)
to candidate GFF2017 Psest_2060 PQQ-dependent dehydrogenase, methanol/ethanol family
Query= CharProtDB::CH_107432 (595 letters) >FitnessBrowser__psRCH2:GFF2017 Length = 592 Score = 1016 bits (2626), Expect = 0.0 Identities = 469/586 (80%), Positives = 534/586 (91%), Gaps = 1/586 (0%) Query: 1 MTRSPRRPMFAVSLVLSAMLLAGAAHAAVSNEEILQDPKNPQQIVTNGLGVQGQRYSPLD 60 M + R FA++ + +A+ ++ AV+++EIL D K+ QIVTNG+G+QGQRYS LD Sbjct: 1 MKHTGLRKPFALTALCAAVAMSSLHAWAVTDQEILNDAKSTDQIVTNGMGLQGQRYSTLD 60 Query: 61 LLNANNVKELRPVWAFSFGGEKQRGQQAQPLIKDGVMYLTGSYSRVFAVDARTGKKLWQY 120 LNANN+ +LRPVW FS GGEKQRGQ+AQPLIKDGVMY+TGSYSRV+AVDARTGK+LWQY Sbjct: 61 ALNANNINQLRPVWGFSLGGEKQRGQEAQPLIKDGVMYITGSYSRVYAVDARTGKELWQY 120 Query: 121 DARLPDDIRPCCDVINRGVALYGDLVFFGTLDAKLVALNKDTGKVVWSKKVADHKEGYSI 180 DARLPD I PCCDVINRGVALY DLV FGTLDAKLVALNKDTGKVVW K VAD+K GYS+ Sbjct: 121 DARLPDGIMPCCDVINRGVALYDDLVIFGTLDAKLVALNKDTGKVVWKKTVADYKAGYSL 180 Query: 181 SAAPMIVNGKLITGVAGGEFGVVGKIQAYNPENGELLWMRPTVEGHMGYVYKDGKAIENG 240 +AAP+IV GKLITGV+GGEFGVVGKI+AYNP+NGELLW RPTVEGHMGYV+KDGK +E+G Sbjct: 181 TAAPLIVKGKLITGVSGGEFGVVGKIEAYNPKNGELLWTRPTVEGHMGYVWKDGKKVESG 240 Query: 241 ISGGEAGKTWPGDLWKTGGAAPWLGGYYDPETNLILFGTGNPAPWNSHLRPGDNLYSSSR 300 ISGGEAGKTWPGDLWKTGGAAPWLGGYYDP+T+ +LFGTGNPAPWNSHLRPGDNLYSSSR Sbjct: 241 ISGGEAGKTWPGDLWKTGGAAPWLGGYYDPDTDSLLFGTGNPAPWNSHLRPGDNLYSSSR 300 Query: 301 LALNPDDGTIKWHFQSTPHDGWDFDGVNELISFNYKDGGKEVKAAATADRNGFFYVLDRT 360 LAL+P+DG+IKWHFQ+TPHDGWD+DGVNEL+SF+Y++GGK +KAA TADRNGFFYVLDRT Sbjct: 301 LALDPNDGSIKWHFQTTPHDGWDYDGVNELVSFDYQEGGKTIKAAGTADRNGFFYVLDRT 360 Query: 361 NGKFIRGFPFVDKITWATGLDKDGRPIYNDASRPGAPG-SEAKGSSVFVAPAFLGAKNWM 419 NGKFIRGFPFVDKITWA GLDK+GRPIY+DA+RPG+P + A+G +V VAP+FLG KNWM Sbjct: 361 NGKFIRGFPFVDKITWAKGLDKNGRPIYDDANRPGSPNDAGAQGKTVTVAPSFLGGKNWM 420 Query: 420 PMAYNKDTGLFYVPSNEWGMDIWNEGIAYKKGAAFLGAGFTIKPLNEDYIGVLRAIDPIS 479 PMAY++DTGLFYVPSNEW MDIWNEG AYKKGAA+LGAGFTI PLN+DYIGVLRAIDP + Sbjct: 421 PMAYSQDTGLFYVPSNEWSMDIWNEGTAYKKGAAYLGAGFTIHPLNDDYIGVLRAIDPKT 480 Query: 480 GKEVWRHKNYAPLWGGVLTTKGNLVFTGTPEGFLQAFNAKTGDKVWEFQTGSGVLGSPVT 539 GKEVWR+KNYAPLWGGVL TKGNLVFTG PEG+L AF+AKTG K++EF TGSGV+GSPVT Sbjct: 481 GKEVWRYKNYAPLWGGVLATKGNLVFTGNPEGYLMAFDAKTGKKLYEFNTGSGVIGSPVT 540 Query: 540 WEMDGEQYVSVVSGWGGAVPLWGGEVAKRVKDFNQGGMLWTFKLPK 585 WEMDGEQYVSV+SGWGGAVPLWGGEVAKRVK+FNQGGM+WTFKLPK Sbjct: 541 WEMDGEQYVSVLSGWGGAVPLWGGEVAKRVKEFNQGGMVWTFKLPK 586 Lambda K H 0.317 0.137 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1937 Number of extensions: 136 Number of successful extensions: 10 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 595 Length of database: 592 Length adjustment: 37 Effective length of query: 558 Effective length of database: 555 Effective search space: 309690 Effective search space used: 309690 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory