Alignments for a candidate for HSERO_RS05250 in Pseudomonas stutzeri RCH2

GapMind for catabolism of small carbon sources

Alignments for a candidate for HSERO_RS05250 in Pseudomonas stutzeri RCH2

Align Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale)
to candidate GFF1357 Psest_1392 ABC-type uncharacterized transport systems, ATPase components

Query= uniprot:D8J111
         (520 letters)



>FitnessBrowser__psRCH2:GFF1357
          Length = 518

 Score =  256 bits (654), Expect = 1e-72
 Identities = 162/492 (32%), Positives = 261/492 (53%), Gaps = 23/492 (4%)

Query: 23  IALRNVCKRFPGVLALDNCQFELAAGEVHALMGENGAGKSTLMKILSGVYQRDSGDILLD 82
           + L  + K++PG LA D     +  GE+HAL+GENGAGKSTLMKI+ GV Q D+G+I   
Sbjct: 9   LQLCGITKQYPGCLANDRIDLSIQPGEIHALLGENGAGKSTLMKIIYGVTQPDAGEIHWQ 68

Query: 83  GKPVEITEPRQAQALGIGIIHQELNLMNHLSAAQNIFIGREPRKAMGLFIDEDELNRQAA 142
           G+ V + +P QA+  GIG++ Q  +L   LS A+NI +    +          +L  +  
Sbjct: 69  GERVTMRDPAQARERGIGMVFQHFSLFETLSVAENIALALGAKAG-----TPKQLEPKIR 123

Query: 143 AIFARMRLDMDPSTPVGELTVARQQMVEIAKALSFDSRVLIMDEPTAALNNAEIAELFRI 202
            +  R  + ++P   V  L++  +Q VEI + L  D R+LI+DEPT+ L   E  ELF  
Sbjct: 124 EVSQRYGMPLEPQRLVHSLSIGERQRVEIIRCLMQDIRLLILDEPTSVLTPQEADELFVT 183

Query: 203 IRDLQAQGVGIVYISHKMDELRQIADRVSVMRDGKYIATVPMQETSMDTIISMMVGRALD 262
           +R L A+G  I++ISHK++E+R +    +V+R G+        E S   +  +MVG A  
Sbjct: 184 LRRLAAEGCSILFISHKLNEVRALCQSATVLRAGRVSGECIPAECSDLELARLMVGDAEG 243

Query: 263 GEQRIPPDTSRNDVVLEVRGLN------RGRAIRDVSFTLRKGEILGFAGLMGAGRTEVA 316
            E   P    R    L V  L+       G ++++V   +R GEI+G AG+ G G+ E+ 
Sbjct: 244 LEAEYPKSEGRAP-FLRVERLSWHNADPFGVSLKEVDLEVRAGEIVGIAGVAGNGQDELL 302

Query: 317 RAIFGADPLEAGE---IIIHGGKAVIKSPADAVAHGIGYLSEDRKHFGLAVGMDVQANIA 373
             + G   L+A +   I   G       P     HG+ ++  +R   G    M +  N  
Sbjct: 303 ALLSGEQRLQAAQAMRIRFLGDDVAHLRPGARRRHGMAFVPAERLGHGAVPSMSLADNGL 362

Query: 374 LSSMGRFTRVGFMDQRAIREA-----AQMYVRQLAIKTPSVEQQARLLSGGNQQKIVIAK 428
           L++   + + G ++Q  IR       A+  +++ A+KTP  +  A  LSGGN QK ++ +
Sbjct: 363 LTA---YQQTGMVEQGLIRRGRVRAFAEQVIQRFAVKTPDAQTPAASLSGGNLQKFILGR 419

Query: 429 WLLRDCDILFFDEPTRGIDVGAKSEIYKLLDALAEQGKAIVMISSELPEVLRMSHRVLVM 488
            +L+   +L    PT G+DVGA + I++ L  L + G AI++IS +L E+ ++S R+  +
Sbjct: 420 EILQQPKLLIAAHPTWGVDVGAAAAIHRALIELRDAGAAILVISEDLEELFQISDRIAAL 479

Query: 489 CEGRITGELARA 500
            +GR++ + A A
Sbjct: 480 SDGRLSPQRATA 491



 Score = 93.6 bits (231), Expect = 2e-23
 Identities = 65/219 (29%), Positives = 110/219 (50%), Gaps = 9/219 (4%)

Query: 292 VSFTLRKGEILGFAGLMGAGRTEVARAIFGADPLEAGEIIIHGGKAVIKSPADAVAHGIG 351
           +  +++ GEI    G  GAG++ + + I+G    +AGEI   G +  ++ PA A   GIG
Sbjct: 27  IDLSIQPGEIHALLGENGAGKSTLMKIIYGVTQPDAGEIHWQGERVTMRDPAQARERGIG 86

Query: 352 YLSEDRKHFGLAVGMDVQANIALSSMGRFTRVGFMDQRAIREAAQMYVRQLAIKTPSVEQ 411
            + +   HF L   + V  NIAL+ +G         +  IRE +Q Y   L       ++
Sbjct: 87  MVFQ---HFSLFETLSVAENIALA-LGAKAGTPKQLEPKIREVSQRYGMPL-----EPQR 137

Query: 412 QARLLSGGNQQKIVIAKWLLRDCDILFFDEPTRGIDVGAKSEIYKLLDALAEQGKAIVMI 471
               LS G +Q++ I + L++D  +L  DEPT  +      E++  L  LA +G +I+ I
Sbjct: 138 LVHSLSIGERQRVEIIRCLMQDIRLLILDEPTSVLTPQEADELFVTLRRLAAEGCSILFI 197

Query: 472 SSELPEVLRMSHRVLVMCEGRITGELARADATQEKIMQL 510
           S +L EV  +     V+  GR++GE   A+ +  ++ +L
Sbjct: 198 SHKLNEVRALCQSATVLRAGRVSGECIPAECSDLELARL 236


Lambda     K      H
   0.320    0.135    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 590
Number of extensions: 39
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 520
Length of database: 518
Length adjustment: 35
Effective length of query: 485
Effective length of database: 483
Effective search space:   234255
Effective search space used:   234255
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources