Alignments for a candidate for SLC26dg in Pseudomonas stutzeri RCH2

GapMind for catabolism of small carbon sources

Alignments for a candidate for SLC26dg in Pseudomonas stutzeri RCH2

Align Fumarate (Na+-independent anion) transporter, SLC26dg of 499 aas and 14 TMSs (characterized)
to candidate GFF4056 Psest_4129 Sulfate permease and related transporters (MFS superfamily)

Query= TCDB::Q1J2S8
         (499 letters)



>FitnessBrowser__psRCH2:GFF4056
          Length = 495

 Score =  581 bits (1498), Expect = e-170
 Identities = 299/494 (60%), Positives = 377/494 (76%), Gaps = 4/494 (0%)

Query: 10  LRQYQREWFANPRKDVLAGIVVALALIPEAIAFSIIAGVDPQVGLYASFIIALITAFLGG 69
           L+  ++ WF+N R DVL+G+VVALALIPEAIAFSIIAGVDP+VGLYASF IA++ AF+GG
Sbjct: 2   LQSIKQNWFSNIRGDVLSGLVVALALIPEAIAFSIIAGVDPRVGLYASFCIAVVIAFVGG 61

Query: 70  RPGMISAATGAMALLMTGLVKDHGIQYLFAATVLTGVLQVVFGWAKLARYLKFVPRSVMV 129
           RPGMISAATGAMALLM  LV++HG+QYL AAT+L G+LQ+  G+ +L   ++FV RSV+ 
Sbjct: 62  RPGMISAATGAMALLMVTLVREHGLQYLLAATLLCGLLQIGAGYLRLGSLMRFVSRSVVT 121

Query: 130 GFVNALAILIFMAQLPQFVGANWQMYAMVAAGLAIIYLLPLV---FKAMPSALVAIVVLT 186
           GFVNALAILIFMAQLP+     W +YAM AAGL IIYL P V    K +PS LV I+ +T
Sbjct: 122 GFVNALAILIFMAQLPELTNVTWHVYAMTAAGLGIIYLFPYVPKLGKVIPSPLVCILSMT 181

Query: 187 VVAVVTGADVKTVGDMGTLPTALPHFQFPQVPLTFETLAIIFPVALTLSLVGLLESLLTA 246
            VAV  G D++TV DMG LP  LP F +P+VPLTFETLAIIFP +  L++VGLLESL+TA
Sbjct: 182 AVAVYFGLDIRTVADMGDLPDTLPVFLWPEVPLTFETLAIIFPYSAALAVVGLLESLMTA 241

Query: 247 QLIDERTDTTSDKNVESRGQGVANIVTGFFGGMAGCAMIGQSMINVTSGGRGRLSTFVAG 306
            ++D+ TDT+SDKN E +GQGV+NIV+G FGGMAGCAMIGQS+INV SGGR RLST +AG
Sbjct: 242 TIVDDLTDTSSDKNRECKGQGVSNIVSGLFGGMAGCAMIGQSVINVKSGGRTRLSTLIAG 301

Query: 307 AFLMVLILALQPLLVQIPMAALVAVMMVVAISTFDWGSLRTLTVFPKGETVVMLATVAVT 366
             L+++++ L   + QIPMAALVAVM++V+I TF W SLR L  +P    +VM+ TV V 
Sbjct: 302 VVLLLMVVFLSDWVGQIPMAALVAVMIMVSIGTFSWDSLRNLKRYPLSTNIVMVVTVVVV 361

Query: 367 VFTHDLSLGVLIGVVLSALFFARKVSQLSQV-TPVDEVDGTRTYRVRGQLFFVSTHDFLH 425
           VFTH+L+ GVL GV+L+A+FFA KV     + + +D     RTY+V GQ+FF S+  F  
Sbjct: 362 VFTHNLAYGVLAGVLLAAMFFANKVGHYLHIGSELDATGNQRTYKVIGQVFFSSSDKFTG 421

Query: 426 QFDFTHPARRVVIDLSDAHFWDGSAVGALDKVMLKFMRQGTSVELRGLNAASATLVERLA 485
            FDF     +V IDLS AHFWD +AV ALDKV++KF R+GT VE+ GLN ASAT+V+R  
Sbjct: 422 AFDFKEALGKVTIDLSRAHFWDITAVAALDKVVIKFRREGTEVEVLGLNEASATIVDRFG 481

Query: 486 VHDKPDALDRMGGH 499
           VHDKP+A+D++  H
Sbjct: 482 VHDKPEAIDKLMSH 495


Lambda     K      H
   0.328    0.140    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 566
Number of extensions: 16
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 499
Length of database: 495
Length adjustment: 34
Effective length of query: 465
Effective length of database: 461
Effective search space:   214365
Effective search space used:   214365
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources