Align Arabinose import ATP-binding protein AraG; EC 7.5.2.12 (characterized, see rationale)
to candidate GFF1357 Psest_1392 ABC-type uncharacterized transport systems, ATPase components
Query= uniprot:B2SYR5 (512 letters) >FitnessBrowser__psRCH2:GFF1357 Length = 518 Score = 237 bits (604), Expect = 9e-67 Identities = 161/493 (32%), Positives = 253/493 (51%), Gaps = 31/493 (6%) Query: 2 SATLRFDNIGKVFPGVRALDGVSFDVNVGQVHGLMGENGAGKSTLLKILGGEYQPDSGRV 61 +A L+ I K +PG A D + + G++H L+GENGAGKSTL+KI+ G QPD+G + Sbjct: 6 TARLQLCGITKQYPGCLANDRIDLSIQPGEIHALLGENGAGKSTLMKIIYGVTQPDAGEI 65 Query: 62 MIDGNEVRFTSAASSIAAGIAVIHQELQYVPDLTVAENLLLGQLPNSLGWVNKREAKRFV 121 G V A + GI ++ Q L+VAEN+ L L G + E K + Sbjct: 66 HWQGERVTMRDPAQARERGIGMVFQHFSLFETLSVAENIALA-LGAKAGTPKQLEPK--I 122 Query: 122 RERLEAMGVALDPNAKLRKLSIAQRQMVEICKALLRNARVIALDEPTSSLSHRETEVLFK 181 RE + G+ L+P + LSI +RQ VEI + L+++ R++ LDEPTS L+ +E + LF Sbjct: 123 REVSQRYGMPLEPQRLVHSLSIGERQRVEIIRCLMQDIRLLILDEPTSVLTPQEADELFV 182 Query: 182 LVRDLRADNRAMIYISHRMDEIYELCDACTIFRDGRKIASHPTLEGVTRDTIVSEMVGRE 241 +R L A+ ++++ISH+++E+ LC + T+ R GR E + + E+ Sbjct: 183 TLRRLAAEGCSILFISHKLNEVRALCQSATVLRAGRVSG-----ECIPAECSDLELARLM 237 Query: 242 ISDIYNYSAR-PLGEVRFAAKGIE----------GHALAQPASFEVRRGEIVGFFGLVGA 290 + D A P E R +E G +L + EVR GEIVG G+ G Sbjct: 238 VGDAEGLEAEYPKSEGRAPFLRVERLSWHNADPFGVSLKE-VDLEVRAGEIVGIAGVAGN 296 Query: 291 GRSELMHLVYGADHKKGGELL---LDGKPIKVRSAGEAIRHGIVLCPEDRKEEGIVAMAT 347 G+ EL+ L+ G + + + G + G RHG+ P +R G V + Sbjct: 297 GQDELLALLSGEQRLQAAQAMRIRFLGDDVAHLRPGARRRHGMAFVPAERLGHGAVPSMS 356 Query: 348 VSENINISCRRHYLRVGM----FLDRKKEAETADRFIKLLKIKTPSRRQKIRFLSGGNQQ 403 +++N ++ Y + GM + R + A++ I+ +KTP + LSGGN Q Sbjct: 357 LADNGLLTA---YQQTGMVEQGLIRRGRVRAFAEQVIQRFAVKTPDAQTPAASLSGGNLQ 413 Query: 404 KAILSRWLAEPDLKVVILDEPTRGIDVGAKHEIYNVIYQLAERGCAIVMISSELPEVLGV 463 K IL R + + K++I PT G+DVGA I+ + +L + G AI++IS +L E+ + Sbjct: 414 KFILGREILQQP-KLLIAAHPTWGVDVGAAAAIHRALIELRDAGAAILVISEDLEELFQI 472 Query: 464 SDRIVVMRQGRIS 476 SDRI + GR+S Sbjct: 473 SDRIAALSDGRLS 485 Score = 82.0 bits (201), Expect = 5e-20 Identities = 59/217 (27%), Positives = 105/217 (48%), Gaps = 11/217 (5%) Query: 276 VRRGEIVGFFGLVGAGRSELMHLVYGADHKKGGELLLDGKPIKVRSAGEAIRHGIVLCPE 335 ++ GEI G GAG+S LM ++YG GE+ G+ + +R +A GI + + Sbjct: 31 IQPGEIHALLGENGAGKSTLMKIIYGVTQPDAGEIHWQGERVTMRDPAQARERGIGMVFQ 90 Query: 336 DRKEEGIVAMATVSENINISCRRHYLRVGMFLDRKKEAETADRFIKLLKIKTPSRRQKIR 395 + +V+ENI ++ +G K+ E R + ++ + Sbjct: 91 ---HFSLFETLSVAENIALA-------LGAKAGTPKQLEPKIREVSQRYGMPLEPQRLVH 140 Query: 396 FLSGGNQQKAILSRWLAEPDLKVVILDEPTRGIDVGAKHEIYNVIYQLAERGCAIVMISS 455 LS G +Q+ + R L + D++++ILDEPT + E++ + +LA GC+I+ IS Sbjct: 141 SLSIGERQRVEIIRCLMQ-DIRLLILDEPTSVLTPQEADELFVTLRRLAAEGCSILFISH 199 Query: 456 ELPEVLGVSDRIVVMRQGRISGELTRKDATEQSVLSL 492 +L EV + V+R GR+SGE + ++ + L Sbjct: 200 KLNEVRALCQSATVLRAGRVSGECIPAECSDLELARL 236 Lambda K H 0.320 0.136 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 654 Number of extensions: 31 Number of successful extensions: 9 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 512 Length of database: 518 Length adjustment: 35 Effective length of query: 477 Effective length of database: 483 Effective search space: 230391 Effective search space used: 230391 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory