Aligments for a candidate for gguA in Pseudomonas stutzeri RCH2

GapMind for catabolism of small carbon sources

Aligments for a candidate for gguA in Pseudomonas stutzeri RCH2

Align GguA aka ATU2347 aka AGR_C_4264, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized)
to candidate GFF1357 Psest_1392 ABC-type uncharacterized transport systems, ATPase components

Query= TCDB::O05176
         (512 letters)



>FitnessBrowser__psRCH2:GFF1357
          Length = 518

 Score =  224 bits (572), Expect = 4e-63
 Identities = 159/494 (32%), Positives = 254/494 (51%), Gaps = 31/494 (6%)

Query: 6   LEMRNITKTFPGVKALENVNLKVKEGEIHALVGENGAGKSTLMKVLSGVYPAGTYEGEIH 65
           L++  ITK +PG  A + ++L ++ GEIHAL+GENGAGKSTLMK++ GV       GEIH
Sbjct: 9   LQLCGITKQYPGCLANDRIDLSIQPGEIHALLGENGAGKSTLMKIIYGVTQPDA--GEIH 66

Query: 66  YEGAVRNFRAINDSEDIGIIIIHQELALVPLLSIAENIFLGNEVASNGVISWQQTFNRTR 125
           ++G     R    + + GI ++ Q  +L   LS+AENI L     +    + +Q   + R
Sbjct: 67  WQGERVTMRDPAQARERGIGMVFQHFSLFETLSVAENIALALGAKAG---TPKQLEPKIR 123

Query: 126 ELLKKVGLKESPETLITDIGVGKQQLVEIAKALSKSVKLLILDEPTASLNESDSEALLNL 185
           E+ ++ G+   P+ L+  + +G++Q VEI + L + ++LLILDEPT+ L   +++ L   
Sbjct: 124 EVSQRYGMPLEPQRLVHSLSIGERQRVEIIRCLMQDIRLLILDEPTSVLTPQEADELFVT 183

Query: 186 LMEFRNQGMTSIIITHKLNEVRKVADQITVLRDGMTVKTLDCHQEEISEDVIIRNMVG-- 243
           L     +G + + I+HKLNEVR +    TVLR G    + +C   E S+  + R MVG  
Sbjct: 184 LRRLAAEGCSILFISHKLNEVRALCQSATVLRAGRV--SGECIPAECSDLELARLMVGDA 241

Query: 244 RDLEDRYPPRDVPIGETILEVKNWNAYHQQHRDRQVLHDINVTVRKGEVVGIAGLMGAGR 303
             LE  YP  +       +E  +W   H        L ++++ VR GE+VGIAG+ G G+
Sbjct: 242 EGLEAEYPKSEGRAPFLRVERLSW---HNADPFGVSLKEVDLEVRAGEIVGIAGVAGNGQ 298

Query: 304 TEFAMSVFG-------KSYGHRITGDVLIDGKPVDVSTVRKAIDA--GLAYVTEDRKHLG 354
            E    + G       ++   R  GD        DV+ +R       G+A+V  +R   G
Sbjct: 299 DELLALLSGEQRLQAAQAMRIRFLGD--------DVAHLRPGARRRHGMAFVPAERLGHG 350

Query: 355 LVLNDNILHN--TTLANLAGVSKASIIDDIKEMKVASDFRTRLRIRSSGIFQETVNLSGG 412
            V + ++  N   T     G+ +  +I   +    A     R  +++        +LSGG
Sbjct: 351 AVPSMSLADNGLLTAYQQTGMVEQGLIRRGRVRAFAEQVIQRFAVKTPDAQTPAASLSGG 410

Query: 413 NQQKVVLSKWLFSNPDVLILDEPTRGIDVGAKYEIYTIINQLAADGKGVLMISSEMPELL 472
           N QK +L + +   P +LI   PT G+DVGA   I+  + +L   G  +L+IS ++ EL 
Sbjct: 411 NLQKFILGREILQQPKLLIAAHPTWGVDVGAAAAIHRALIELRDAGAAILVISEDLEELF 470

Query: 473 GNCDRIYVMNEGRI 486
              DRI  +++GR+
Sbjct: 471 QISDRIAALSDGRL 484



 Score = 63.5 bits (153), Expect = 2e-14
 Identities = 54/227 (23%), Positives = 105/227 (46%), Gaps = 11/227 (4%)

Query: 283 INVTVRKGEVVGIAGLMGAGRTEFAMSVFGKSYGHRITGDVLIDGKPVDVSTVRKAIDAG 342
           I+++++ GE+  + G  GAG++     ++G +      G++   G+ V +    +A + G
Sbjct: 27  IDLSIQPGEIHALLGENGAGKSTLMKIIYGVTQPD--AGEIHWQGERVTMRDPAQARERG 84

Query: 343 LAYVTEDRKHLGLVLNDNILHNTTLANLAGVSKASIIDDIKEMKVASDFRTRLRIRSSGI 402
           +  V +   H  L    ++  N  LA  A       ++  K  +V+  +   L  +    
Sbjct: 85  IGMVFQ---HFSLFETLSVAENIALALGAKAGTPKQLEP-KIREVSQRYGMPLEPQ---- 136

Query: 403 FQETVNLSGGNQQKVVLSKWLFSNPDVLILDEPTRGIDVGAKYEIYTIINQLAADGKGVL 462
            +   +LS G +Q+V + + L  +  +LILDEPT  +      E++  + +LAA+G  +L
Sbjct: 137 -RLVHSLSIGERQRVEIIRCLMQDIRLLILDEPTSVLTPQEADELFVTLRRLAAEGCSIL 195

Query: 463 MISSEMPELLGNCDRIYVMNEGRIVAELPKGEASQESIMRAIMRSGE 509
            IS ++ E+   C    V+  GR+  E    E S   + R ++   E
Sbjct: 196 FISHKLNEVRALCQSATVLRAGRVSGECIPAECSDLELARLMVGDAE 242


Lambda     K      H
   0.316    0.135    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 548
Number of extensions: 25
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 512
Length of database: 518
Length adjustment: 35
Effective length of query: 477
Effective length of database: 483
Effective search space:   230391
Effective search space used:   230391
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources